Thin-layer chromatographic and high-performance thin-layer chromatographic ready-for-use preparations with concentrating zones

Halpaap, H.; Krebs, K.-F.

doi:10.1016/s0021-9673(01)92089-8

Cited by 35 publications

(4 citation statements)

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“…Thin-Layer Chromatography. A 20-µ portion of sample was applied to the preadsorbent layer via a microliter syringe, taking care to apply the sample in one smooth continuous stroke to maximize uniformity of concentration across the band (Halpaap and Krebs, 1977). The plate was developed in a saturated tank containing chloroform-tert-butanol (80:20) and then dried in an oven at 100 °C for 1 min.…”

Section: Methodsmentioning

confidence: 99%

Determination of sulfamethazine in swine tissues by quantitative thin-layer chromatography

Thomas¹,

Soroka²,

Simpson³

et al. 1981

J. Agric. Food Chem.

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Section: Methodsmentioning

confidence: 99%

Determination of sulfamethazine in swine tissues by quantitative thin-layer chromatography

Thomas¹,

Soroka²,

Simpson³

et al. 1981

J. Agric. Food Chem.

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“…Attaching a highly porous pre-concentration zone [27] to a GLAD-UTLC plate may permit focusing of our broad spots into narrow bands prior to separation. Our media could also be amenable to the programmed multiple development technique [28].…”

Section: Potential Opportunitiesmentioning

confidence: 99%

Analyte migration in anisotropic nanostructured ultrathin-layer chromatography media

Oko

Jim

Taschuk

et al. 2011

Journal of Chromatography A

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“…The technique of using a narrow Kieselguhr concentrating layer was first described by Abbott and Thomson (13), and is based on the difference in adsorbing power of the two sorbents. Halpaap and Krebs (14) were the first to study thoroughly a commercial Silica Gel preabsorbent or sample concentrating zone both for conventional and HPTLC plates. This zone extends across the width of the plate for about 25 mm in the direction of development and is about 150 pm thick.…”

Section: Sample Spottingmentioning

confidence: 99%

“…Halpaap and Krebs (14) used the separation number of Kaiser (15) to compare the performance of precoated Silica Gel 60 with and without a concentrating zone, and precoated Silica Gel 60 HPTLC plates with and without a concentrating zone. The separation number gives the maximum number of completely separated substances in the range from R{ = 0 to Rf = 1, where separation is considered complete when the interval between the concentration maxima is equal to or greater than the sum of the two peak widths at half-height.…”

Section: Sample Spottingmentioning

confidence: 99%

High performance thin layer chromatography

Costanzo¹

1984

J. Chem. Educ.

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One of the first points made in most analytical chemistry texts is the distinction between the qualitative and quantitative aspects of chemical analysis. However, the problems of detecting, identifying, and determining the amounts of sample constituents are not always the initial issue of concern. Often some form of preliminary separation must take place. Increasingly, the principles of chromatography are being introduced in many undergraduate science courses. This paper will attempt to clarify where in the scheme of modern chromatography high performance thin layer chromatography fits, and why in some situations it is a viable alternative to gas and high performance liquid chromatography.Until the middle of this century, the separation of a sample into its various components was accomplished in painfully slow ways, such as paper or column chromatography. This is definitely not the situation today. Beginning with the advent of practical gas chromatography in the 1950's, high efficiency in sample separation became commonplace. After the pioneering work of Justus Kirchner and Egon Stahl, thin layer chromatography (TLC) became important for the separation of samples not amenable to analysis by gas chromatography. The rapid growth of TLC was slowed during the 1970's with the corresponding rise in popularity of high pressure liquid chromatography (HPLC). Although both HPLC and TLC are capable of separating the same type of compounds, HPLC is considerably more efficient. Further, capacity factors in HPLC are more reproducible than R{ values in TLC. However, recent improvements in TLC have removed many of its limitations, and in some situations, it actually gives better results than HPLC. To emphasize the fact that TLC may be a legitimate alternative to HPLC, the relatively new term high performance thin layer chromatography (HPTLC) has been introduced.Just what is high performance thin layer chromatography (1-4)1 How is it different from classical TLC? To what extent does HPTLC keep the advantages of classical TLC, such as minimal sample clean up, flexibility in solvent selection and sample detection, high sample through put, and low analysis costs?Many of the recent improvements in TLC may be placed under the heading of high performance thin layer chromatography, as a means of emphasizing improved efficiency and precision. Zlatkis and Kaiser (J) define HPTLC as a combination of improvements in several aspects of TLC. These improvements include optimized plate coating materials, a new method of developing the mobile phase, the ways in which samples are applied to the plate, and a novel procedure for conditioning the TLC plate. A more concise definition of high performance is given by Jupille (2). It was pointed out that for a given chromatographic system resolution of sample components are dependent upon selectivity and efficiency.

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Thin-layer chromatographic and high-performance thin-layer chromatographic ready-for-use preparations with concentrating zones

Cited by 35 publications

References 1 publication

Determination of sulfamethazine in swine tissues by quantitative thin-layer chromatography

Determination of sulfamethazine in swine tissues by quantitative thin-layer chromatography

Analyte migration in anisotropic nanostructured ultrathin-layer chromatography media

High performance thin layer chromatography

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