Thin film preparation of polyphenol oxidase enzyme (PPO) and investigation of its organic vapor interaction mechanism
M Evyapan,
D E Deniz
Abstract:This study investigated spin coated thin films of polyphenol oxidase enzyme (PPO) as vapor sensor to detect chloroform, acetone, ethyl acetate, isopropyl alcohol and toluene. Thin film of enzyme was produced onto a bio-composite (gelatine chitosan) first layer via 5000 rpm spin speed. UV-visible spectroscopy and Quartz Crystal Microbalance (QCM) measurements were carried out to analyze the reproducibility of PPO spun film. It was found that the PPO enzyme can be transferred onto a solid substrate as a solid s… Show more
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