Thidiazuron-induced abnormalities in plant tissue cultures

Dewir, Yaser Hassan; Nurmansyah, Nurmansyah; Naidoo, Yougasphree; Silva, Jaime A. Teixeira da

doi:10.1007/s00299-018-2326-1

Cited by 113 publications

(68 citation statements)

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“…Meanwhile, TDZ-treated Philodendron 'Imperial Red' and 'Imperial Rainbow' explants were reported to form numerous leaf-like structures (Chen et al, 2012). Dewir et al (2018) suggested that TDZ-induced abnormalities and inhibition of shoot proliferation are due to overdose or continuous exposure for a long duration until organogenesis. BAP was more effective for inducing the shoot multiplication of lacy tree philodendron than the other cytokinins, as previously reported for other Philodendron species (Gangopadhyay et al, 2004;Han and Park, 2008; Jambor-Benczur and Marta-Riffer, 1990; Sreekumar et al, 2001).…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of Lacy Tree Philodendron (Philodendron bipinnatifidum Schott ex Endl.)

Alawaadh¹,

Dewir²,

Alwihibi³

et al. 2020

horts

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The present study aimed to optimize the micropropagation of lacy tree philodendron using shoot tip explants. Axillary shoot regeneration was investigated in Murashige and Skoog (MS) medium with different types and concentrations of plant growth regulators, varied levels of MS medium salt strength, sucrose concentration, and light intensity and culture type. Adding 6-benzylaminopurine (BAP; 1 mg·L−1) significantly increased shoot multiplication compared with other cytokinins, and the combination of cytokinins and auxins [indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA)], yielded more shoots than cytokinins alone, with the greatest number of axillary shoots (11.4 per explant) obtained using both BAP (1 mg·L−1) and IBA (0.5 mg·L−1). In addition, the use of half-strength salt concentrations significantly reduced shoot multiplication, and high sucrose concentrations (>30 g·L−1) reduced explant growth. High light intensity also reduced shoot multiplication and growth, owing to photoinhibition, and shoot multiplication was more efficient in gelled culture, whereas shoot growth was greater in liquid/bioreactor culture. The best rooting success (100%) and greatest root number and fresh weight were obtained using MS medium supplemented with NAA (1–2 mg·L−1). The resulting plantlets were successfully acclimatized, with a survival rate of 100%, and were morphologically similar to the mother plant.

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Section: Resultsmentioning

confidence: 99%

Micropropagation of Lacy Tree Philodendron (Philodendron bipinnatifidum Schott ex Endl.)

Alawaadh¹,

Dewir²,

Alwihibi³

et al. 2020

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“…1e). This fortified the existence of TDZ-induced abnormalities in tissue culture 27 . Our results show that new shoots could not develop in the presence of 2,4-D.…”

Section: Discussionmentioning

confidence: 96%

Adventitious shoot organogenesis from leaf explants of Portulaca pilosa L.

Chen

Xiong

et al. 2020

Sci Rep

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This study established, for the first time, shoot proliferation and plant regeneration protocols via shoot organogenesis from leaf explants of a medical and ornamental plant, Portulaca pilosa L. the optimal proliferation of axillary shoots was 6.2-fold within 30 days on Murashige and Skoog (MS) medium supplemented with 3.0 µM 6-benzyladenine (BA). Shoots could be induced directly from leaf explants, forming an average of 3.8 adventitious shoots per explant, on optimal MS medium supplemented with 1.0 µM thidiazuron (TDZ) and 0.1 µM α-naphthaleneacetic acid (NAA). A higher concentration of TDZ (3.0 µM), alone or in combination with 0.1 µM NAA, induced somatic embryo-like shoot buds and then developed into real shoots. Rooting was easier since roots were induced on all rooting media within one month. Half-strength MS medium free of plant growth regulators was best for rooting. Rooted plantlets were transferred to a sand: perlite (1:1, v/v) substrate, resulting in highest survival (90%). Plantlets showed more robust growth, however, on substrates of yellow mud: perlite (1:1, v/v) or peat soil: vermiculite: perlite (1:1:1, v/v).Portulacaceae, consisting of annual or perennial plants and distributed in temperate and tropical regions of the world, is one of 19 families of terrestrial plants that display C 4 photosynthesis 1,2 . Portulaca pilosa L. (Portulacaceae) is an annual herb native to Asia but that spread to North and South America 3,4 . In China, P. pilosa is distributed only in southern provinces where it grows in the wild on seashores, in orchards, wastelands, and roadsides. A diterpenoid, pilosanone C, was isolated from the shoots and roots of P. pilosa 5,6 . P. pilosa contains a variety of chemical components, including polyphenols, flavonoids, sugars, organic acids, steroids, tannins, steroids, and others, but the highest content is of flavonoids and polyphenols explaining its high antioxidant activity and thus high toxicity to tumor cells 7,8 . It is commonly used as a traditional remedy to treat antipyresis and analgesia and serves as a hepato-protective, anti-diarrheal, and diuretic for healing burns, erysipelas, and injuries 9 . Phytochemical screening revealed the presence of reducing sugars, phenols, tannins, steroids, terpenoids, cardiac glycosides, and carotenoids in the ethanolic extract of dried aerial parts of P. pilosa, which also demonstrated an antimicrobial effect against Pseudomonas aeruginosa 7,10 . In addition, P. pilosa, which has red-purple flowers that bloom over a long flowering period, is regarded as an excellent ornamental succulent plant 11 .The capsules of P. pilosa, which is autogamous and self-compatible, yield a large number of seeds that require light and 25 °C for maximum germination 12 . P. pilosa seeds show no dormancy and poor viability in long-term storage 13 . Therefore, seeds need to be sown as quickly as possible when they mature. In fact, in natural conditions in the wild, it is not always possible to attain suitable seed germination conditions related to soil, light, t...

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“…This was followed by Rio (7.2% of regenerating explants using the WU protocol), and Pink Kafir (23.7% of regenerating explants using the WU protocol), thus illustrating the high susceptibility of Pink Kafir to mutagenesis during in vitro development. This impact of genotype on albino regeneration has been noted during other in vitro regeneration studies (Ayed et al 2010; Khatun et al 2010; Park et al 2013), as has the impact of the in vitro protocol used (Khatun et al 2010; Park et al 2013; Sriskandarajah et al 2015; Dewir et al 2018).…”

Section: Discussionmentioning

confidence: 56%

“…As explants cultured using both protocols were incubated in the same types of culture vessels and the same tissue culture chamber, our evidence of the WU protocol being more promotive of albino regeneration suggested that medium composition played a role in this response. Previous studies have shown that inclusion of casein hydrolysate reduced albinism during in vitro regeneration (Sriskandarajah et al 2015), while the use of maltose as a carbohydrate source (Park et al 2013), TDZ as a cytokinin source (Dewir et al 2018) and copper supplements (Makowska et al 2017) promoted albinism. While the WU protocol was more supportive of overall plantlet regeneration than LG, this protocol contained almost 8-fold higher levels of copper sulfate in both CIM and RM formulations, as well as the use of maltose in CIM, and TDZ in RM.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of In Vitro Responses and Regeneration Between Diverse Bioenergy Sorghum Genotypes

Flinn

Dale

Disharoon

et al. 2019

Preprint

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25Sorghum has been considered a recalcitrant plant in vitro, and suffers from a lack of 26 regeneration protocols that function broadly and efficiently across a range of genotypes. This study 27 was initiated to identify differential genotype-in vitro protocol responses across a range of 28 bioenergy sorghum bioenergy parental lines, in order to characterize response profiles for use in 29 future genetic studies. Seven bioenergy sorghum genotypes were compared, along with the 30 common grain sorghum genotype Tx430, for their in vitro regeneration responses using two 31 different in vitro protocols, LG and WU. All genotypes displayed some level of response during 32 in vitro culture with both protocols. Distinct genotype-protocol responses were observed, with the 33 WU protocol significantly better for plantlet regeneration. All bioenergy genotypes, with the 34 exception of Chinese Amber, performed as well, if not better than Tx430, with Rio and PI329311 35 the top regenerating lines. Genotypes displayed protocol-dependent, differential phenolic 36 exudation responses, as indicated by medium browning. During the callus induction phase, 37 genotypes prone to medium browning exhibited a response on WU medium which was either equal 38 or greater than on LG medium, with Pink Kafir and PI329311 the most prone to medium browning. 39 Genotype-and protocol-dependent albino plantlet regeneration was also noted, with three of the 40 bioenergy genotypes showing albino plantlet regeneration. Grassl, Rio and Pink Kafir were 41 susceptible to albino plantlet regeneration, with the response strongly associated with the WU 42 protocol. Pink Kafir displayed the highest albino formation, with close to 25% of regenerating 43 explants forming albino plantlets. 44 45 46 47 48 49 50 51 52 53 Sorghum [Sorghum bicolor (L.) Moench] ranks fifth of the major grain crops in production, 54 area harvested, and yield worldwide (FAOSTAT Database 2017), and more than 300 million 55 people use it as a staple food, particularly in developing semiarid tropical regions (Kebede et al.56

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Thidiazuron-induced abnormalities in plant tissue cultures

Cited by 113 publications

References 122 publications

Micropropagation of Lacy Tree Philodendron (Philodendron bipinnatifidum Schott ex Endl.)

Micropropagation of Lacy Tree Philodendron (Philodendron bipinnatifidum Schott ex Endl.)

Adventitious shoot organogenesis from leaf explants of Portulaca pilosa L.

Comparative Analysis of In Vitro Responses and Regeneration Between Diverse Bioenergy Sorghum Genotypes

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