Thickness and Surface Density of Extracellular Polymers on
            <i>Acidithiobacillus ferrooxidans</i>

Taylor, Eric S.; Lower, Steven K.

doi:10.1128/aem.01904-07

Cited by 28 publications

(30 citation statements)

References 22 publications

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“…Grafting densities resulted from fitting the steric model to the approach curves collected on Acidithiobacillus ferrooxidans were estimated to be between 3.4×10 16 and 7.1×10 16 moleules/m 254 . Our steric model results previously obtained on the pathogenic L. monocytogenes EGDe grown in BHIB adjusted to a pH value of 6 indicated a grafting density of 2.26× 10 16 ± 0.49 × 10 16 biopolymers/m 2 and a brush thickness of 175 ± 45 nm, r 2 = 0.96 ± 0.04.…”

Section: Resultsmentioning

confidence: 99%

The role of the pH conditions of growth on the bioadhesion of individual and lawns of pathogenic Listeria monocytogenes cells

Park

Abu-Lail

2011

Journal of Colloid and Interface Science

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The work of adhesion that governs the interactions between pathogenic Listeria monocytogenes and silicon nitride in water was probed for individual cells using atomic force microscopy and for lawns of cells using contact angle measurements combined with a thermodynamic-based harmonic mean model. The work of adhesion was probed for cells cultured under variable pH conditions of growth that ranged from pH 5 to pH 9. Our results indicated that L. monocytogenes cells survived and adapted well to the chemical stresses applied. For all pH conditions investigated, a transition was observed in the generation time, physiochemical properties, biopolymer grafting density and bioadhesion for cells cultured in media adjusted to pH 7 of growth. In media with pH 7, the generation time for the bacterial cells was lowest, the specific growth rate constant was highest, the cells were the most polar, cells displayed the highest grafting density of surface biopolymers and the highest bioadhesion to silicon nitride in water represented in terms of the work of adhesion. When compared, the work of adhesion values quantified between silicon nitride and lawns of L. monocytogenes cells were linearly correlated with the work of adhesion values quantified between silicon nitride and individual L. monocytogenes cells.

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Section: Resultsmentioning

confidence: 99%

The role of the pH conditions of growth on the bioadhesion of individual and lawns of pathogenic Listeria monocytogenes cells

Park

Abu-Lail

2011

Journal of Colloid and Interface Science

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“…Electron tunneling over distances <2 nm is widely accepted [341,363] and would allow both EPS complexed and solution Fe(III) to be reduced. However, given the thickness of the EPS is 10-100 nm wide, not all Fe(III) would be within range, requiring diffusion towards the surface [286,364].…”

Section: Attachment Of Extreme Thermoacidophiles To Surfacesmentioning

confidence: 99%

The Confluence of Heavy Metal Biooxidation and Heavy Metal Resistance: Implications for Bioleaching by Extreme Thermoacidophiles

et al. 2015

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Extreme thermoacidophiles (Topt > 65 °C, pHopt < 3.5) inhabit unique environments fraught with challenges, including extremely high temperatures, low pH, as well as high levels of soluble metal species. In fact, certain members of this group thrive by metabolizing heavy metals, creating a dynamic equilibrium between biooxidation to meet bioenergetic needs and mechanisms for tolerating and resisting the toxic effects of solubilized metals. Extremely thermoacidophilic archaea dominate bioleaching operations at elevated temperatures and have been considered for processing certain mineral types (e.g., chalcopyrite), some of which are recalcitrant to their mesophilic counterparts. A key issue to consider, in addition to temperature and pH, is the extent to which solid phase heavy metals are solubilized and the concomitant impact of these mobilized metals on the microorganism's growth physiology. Here, extreme thermoacidophiles are examined from the perspectives of biodiversity, heavy metal biooxidation, metal resistance mechanisms, microbe-solid interactions, and application of these archaea in biomining operations.

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“…Atomic force microscope (AFM) has emerged as a powerful tool for imaging biological samples at nanoscale without destroying the specimens. AFM has been used for characterizing the physical properties of microbial biofilms (Auerbach et al 2000), cell surface properties of fungi and bacteria (Adya et al 2006;Liu et al 2006), properties of microbial EPS (Taylor and Lower 2008), and single molecular recognition and interaction (Hinterdorfer and Dufrene 2006). Here, we extend the use of AFM to characterize the changes occurring on the surface of native unsaturated biofilms underwent different environmental stresses.…”

Section: Introductionmentioning

confidence: 97%

Responses of unsaturated Pseudomonas putida CZ1 biofilms to environmental stresses in relation to the EPS composition and surface morphology

Lin

Chen

Long

et al. 2014

World J Microbiol Biotechnol

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The extracellular polymeric substance (EPS) and surface properties of unsaturated biofilms of a heavy metal-resistant rhizobacterium Pseudomonas putida CZ1, in response to aging, pH, temperature and osmotic stress, were studied by quantitative analysis of EPS and atomic force microscope. It was found that EPS production increased approximately linearly with culture time, cells in the air-biofilm interface enhanced EPS production and decreased cell volume to cope with nutrient depletion during aging. Low pH, high temperature and certain osmotic stress (120 mM NaCl) distinctly stimulated EPS production, and the main component enhanced was extracellular protein. In addition to the enhancement of EPS production in response to high osmotic (328 mM NaCl) stress, cells in the biofilm adhere tightly together to maintain a particular microenvironment. These results indicated the variation of EPS composition and the cooperation of cells in the biofilms is important for the survival of Pseudomonas putida CZ1 from environmental stresses in the unsaturated environments such as rhizosphere.

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Thickness and Surface Density of Extracellular Polymers on Acidithiobacillus ferrooxidans

Cited by 28 publications

References 22 publications

The role of the pH conditions of growth on the bioadhesion of individual and lawns of pathogenic Listeria monocytogenes cells

The role of the pH conditions of growth on the bioadhesion of individual and lawns of pathogenic Listeria monocytogenes cells

The Confluence of Heavy Metal Biooxidation and Heavy Metal Resistance: Implications for Bioleaching by Extreme Thermoacidophiles

Responses of unsaturated Pseudomonas putida CZ1 biofilms to environmental stresses in relation to the EPS composition and surface morphology

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