Thickness and density of protein films by optical mixing spectroscopy

Uzgiris, E. E.; Fromageot, H. P. M.

doi:10.1002/bip.1976.360150204

Cited by 45 publications

(6 citation statements)

References 16 publications

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“…In addition to their fidelity with the measured mobilityionic strength curve, further evidence supporting the predictions of our model comes from Uzgiris and Fromageot (1976), who measured the hydrodynamic thickness of protein films adsorbed on polystyrene latex spheres. The FIGURE 7 Plot of ratio u(z)/E (fluid velocity per electric field) in gm s-V`cm, as a function of z, where u(z) is given by Eqs.…”

Section: Numerical Results and Discussionsupporting

confidence: 76%

Theory of the electrokinetic behavior of human erythrocytes

Levine¹,

Levine²,

Sharp³

et al. 1983

Biophysical Journal

251

199

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We develop a theory of electrophoresis of human erythrocytes that predicts mobilities significantly smaller than those based on the classical Smoluchowski relation. In the classical treatment the charge is assumed to be spread uniformly on the hydrodynamic surface. The present model takes into account that most of the charge, due mainly to sialic acid, is contained in the glycocalyx. The glycocalyx is modeled as a permeable layer of polyelectrolyte molecules anchored to the cell membrane. The charge is assumed to be uniformly distributed throughout this layer. The fluid flow in the layer is treated as being dominated by Stokes friction arising from idealized polymer segments. The Navier-Stokes equations are solved to give the dependence of electroosomotic velocity with distance from the cell surface. An expression for the electrophoretic mobility is obtained which contains two parameters (a) the thickness of the glycocalyx and (b) the mean polymer segment radius. The best fit to experimental data is obtained if these are given the values 75 A and 7 A, respectively. Deviation from experimental data at low ionic strength (less than 0.05 M) occurs. However, this deviation is in the direction one would expect if at low ionic strength the polyelectrolyte layer expands slightly due to decreased charge shielding.

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Section: Numerical Results and Discussionsupporting

confidence: 76%

Theory of the electrokinetic behavior of human erythrocytes

Levine¹,

Levine²,

Sharp³

et al. 1983

Biophysical Journal

251

199

View full text Add to dashboard Cite

show abstract

“…This equation is valid for d A <£ s. It will be noticed that there is a contradiction between the condition s <^ A used in obtaining equations (19)(20), and the condition d A -4 s. The first implies that the colloidal particles should have a radius less than A/20 ~ 30 nm, which is not much bigger than the value of d A expected from proteins ~ 10 nm in diameter (and which, moreover, may lead to the complications in the adsorption behaviour referred to earlier 3 ). For larger particles the full Mie theory should be used to obtain equations corresponding to equations (19) to (20); numerical simulations show, however, that the error introduced through using equation (19) may be less than typical experimental errors (Uzgiris & Fromageot, 1976). Sedimentation.…”

Section: Jjramsdenmentioning

confidence: 99%

“…For measuring D of small colloidal particles a million channels of duration o-i ms would be typical measurement parameters. Uzgiris & Fromageot (1976) have pointed out that the thickness d A which is required in order to be able to determine 8 or F from D (equations 11-14) can be obtained from the way the scattered light intensity / s changes as a film is adsorbed onto the particle surface. Provided that s <£ A, the polarizability a F of the naked sphere is, according to the Rayleigh approximation, a F = 5 3 (n F -n%)Hn% + zn%).…”

Section: The Diffusivity Of Colloidal Spheresmentioning

confidence: 99%

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Experimental methods for investigating protein adsorption kinetics at surfaces

Ramsden¹

1994

Quart. Rev. Biophys.

177

131

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The adsorption of proteins at the solid-liquid interface is a process of fundamental importance in nature. Extensive reviews (MacRitchie, 1978; Andrade & Hlady, 1986; Norde, 1986) testify to the strong interest which has been shown in the problem during the past few decades. Norde & Lyklema (1978) have rightly pointed out that protein adsorption is scientifically intriguing; the phenomenology is complicated and includes many presently apparently irreconcilable observations.

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“…A number of investigations have been made on protein adsorption on at solid-liquid interfaces by estimating the free energy of protein adsorption and determining its adsorption mode (Lyman et al, 1968;Dillonman and Miller, 1973;Nyilas et al, 1974;Lee and Kim, 1974;Brash et al, 1974;Chiu et al, 1976;Uzgiris and Fromageot, 1976;Norde and Lyklema, 1978a-e;Soberquist and Walton, 1980;Bagchi and Birnbaum, 1981;Chan and Brash, 1981;Dulm et al, 1981;Dulm and Norde, 1983;Schmidt et al, 1983;Shirahama and Shuzawa, 1985).…”

Section: Blood Protein-solid Adsorptionmentioning

confidence: 99%

Self-Assembly Monolayer Structures of Lipids and Macromolecules at Interfaces

Birdi¹

2002

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PREFACEThe information that science can extract from molecules that are essential for the processes of life can lead to better understanding of those processes. In natural selection during evolution (both prebiotic and biotic), physicochemical forces have been featured. A specific class of molecules, the amphiphiles, in aqueous environments exhibit self-assembly properties at liquid and solid interfaces. Such properties when combined with other forces require a special analysis. Of the self-assembly properties of amphiphiles, one is the formation of monomolecular films at both liquid-air and solid-air interfaces. During the last few decades, much has been reported in the literature on the formation of insoluble self-assembly monolayer (SAM) films of lipids and macromolecules (synthetic polymers and biopolymers) on the surface of water or at oil-water interfaces.The phenomenon of monolayer self-assembly has attracted considerable attention as a means of controlling molecular structures at interfaces. Studies of monomolecular films have been found to provide much physicochemical information on a molecular scale, which is useful for understanding many industrial and biological phenomena. For instance, the information obtained from lipid monolayer studies has been useful in determining the forces that stabilize various SAM structures found in lipid bilayers, vesicles, biological cell membranes, virus-cell fusion, emulsions, foams and films, and other self-assemblies. Current texts generally devote a single chapter to the characteristics of spread monolayers on liquids, and a researcher may have to look up a few hundred references to determine the procedures needed to investigate or analyze a particular phenomenon. Furthermore, there is an urgent need at this time for a text that discusses the state of the art regarding the surface phenomena exhibited by lipids and biopolymers, as they are relevant to a wide variety of surface and interfacial processes. During the last decade, the study of SAMs on solid surfaces has been aided greatly by developments in scanning tunneling microscopy (STM) and atomic force microscopy (AFM).The purpose of this book is to bring the reader up to date with the most recent experimental and the theoretical developments in relation to SAMs at liquid and solid surfaces. It is my intention to lead the researcher through the vast literature in such a way that he or she will be able to pursue particular investigation with suitable guidance. Such investigations may be industrial (emulsions, foams, dispersions, enhanced oil recovery, nonlinear optics) or biological [lipid-phase transitions, vesicles (drug targeting); bilayers, membranes, ion transport through membranes, sensors] in nature. The treatment in different parts of the text is based on the fundamental concepts of classical thermodynamics of surfaces (interfaces), i.e., the Gibbs adsorption theory. A large amount of experimental data from the literature has been included and critically examined from a vii viii PREFACE theoretical viewpoint...

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Thickness and density of protein films by optical mixing spectroscopy

Cited by 45 publications

References 16 publications

Theory of the electrokinetic behavior of human erythrocytes

Theory of the electrokinetic behavior of human erythrocytes

Experimental methods for investigating protein adsorption kinetics at surfaces

Self-Assembly Monolayer Structures of Lipids and Macromolecules at Interfaces

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