Thiamine induces resistance in tobacco against black shank

Suohui, Tian; Chen, Yanping; Zi, Shuhui; Li, Zhihua; Honggang, Jin; Liu, Jun; Liu, Tao

doi:10.1007/s13313-021-00848-3

Cited by 5 publications

(4 citation statements)

References 53 publications

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“…Thiamine is a B complex vitamin that is produced by plants and microorganisms. Studies have shown that a thiamine treatment has a significant inhibitory effect on rice sheath blight [ 45 ], soybean carbon rot [ 46 ], tobacco black shank [ 47 ], and grape pulp mold [ 48 ]. Specifically, fungal growth is reportedly limited to the infection site, which may be because thiamine, as a defense activator, induces rapid opening of defense response [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

“…Specifically, fungal growth is reportedly limited to the infection site, which may be because thiamine, as a defense activator, induces rapid opening of defense response [ 49 ]. Thiamine-induced defense reactions include the production of H 2 O 2 , the synthesis of callose, the expression of defense-related genes, the accumulation of phenols, and the death of HR-like cells [ 47 , 49 ]. In the current study, the thiamine metabolism KEGG pathway was enriched among the DEGs at 36 and 60 hpi in ZH (Fig.…”

Section: Discussionmentioning

confidence: 99%

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Cytological and transcriptomic analysis to unveil the mechanism of web blotch resistance in Peanut

Wu,

Sun,

et al. 2023

BMC Plant Biol

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Background Peanut is an important oil crop worldwide. Peanut web blotch is a fungal disease that often occurs at the same time as other leaf spot diseases, resulting in substantial leaf drop, which seriously affects the peanut yield and quality. However, the molecular mechanism underlying peanut resistance to web blotch is unknown. Results The cytological examination revealed no differences in the conidium germination rate between the web blotch-resistant variety ZH and the web blotch-susceptible variety PI at 12–48 hpi. The appressorium formation rate was significantly higher for PI than for ZH at 24 hpi. The papilla formation rate at 36 hpi and the hypersensitive response rate at 60 and 84 hpi were significantly higher for ZH than for PI. We also compared the transcriptional profiles of web blotch-infected ZH and PI plants at 0, 12, 24, 36, 48, 60, and 84 hpi using an RNA-seq technique. There were more differentially expressed genes (DEGs) in ZH and PI at 12, 36, 60, and 84 hpi than at 24 and 48 hpi. Moreover, there were more DEGs in PI than in ZH at each time-point. The analysis of metabolic pathways indicated that pantothenate and CoA biosynthesis; monobactam biosynthesis; cutin, suberine and wax biosynthesis; and ether lipid metabolism are specific to the active defense of ZH against YY187, whereas porphyrin metabolism as well as taurine and hypotaurine metabolism are pathways specifically involved in the passive defense of ZH against YY187. In the protein-protein interaction (PPI) network, most of the interacting proteins were serine acetyltransferases and cysteine synthases, which are involved in the cysteine synthesis pathway. The qRT-PCR data confirmed the reliability of the transcriptome analysis. Conclusion On the basis of the PPI network for the significantly enriched genes in the pathways which were specifically enriched at different time points in ZH, we hypothesize that serine acetyltransferases and cysteine synthases are crucial for the cysteine-related resistance of peanut to web blotch. The study results provide reference material for future research on the mechanism mediating peanut web blotch resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cytological and transcriptomic analysis to unveil the mechanism of web blotch resistance in Peanut

Wu,

Sun,

et al. 2023

BMC Plant Biol

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“…In previous experiments, we isolated and maintained the P. nicotianae strain, and the P. nicotianae strain was sequenced using a polymerase chain reaction and compared with sequences in the National Center for Biotechnology Information databases 58 . The strain was routinely recultured on potato dextrose agar (PDA: 200 g of potato, 20 g of glucose and 15 g of agar in 1000 mL water) at 27 ℃ in the dark for 15 days 59 .…”

Section: Methodsmentioning

confidence: 99%

Lactic acid induced defense responses in tobacco against Phytophthora nicotianae

Yan,

Ma,

Peng

et al. 2024

Sci Rep

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Induced resistance is considered an eco-friendly disease control strategy, which can enhance plant disease resistance by inducing the plant’s immune system to activate the defense response. In recent years, studies have shown that lactic acid can play a role in plant defense against biological stress; however, whether lactic acid can improve tobacco resistance to Phytophthora nicotianae, and its molecular mechanism remains unclear. In our study, the mycelial growth and sporangium production of P. nicotianae were inhibited by lactic acid in vitro in a dose-dependent manner. Application of lactic acid could reduce the disease index, and the contents of total phenol, salicylic acid (SA), jasmonic acid (JA), lignin and H2O2, catalase (CAT) and phenylalanine ammonia–lyase (PAL) activities were significantly increased. To explore this lactic acid-induced protective mechanism for tobacco disease resistance, RNA-Seq analysis was used. Lactic acid enhances tobacco disease resistance by activating Ca2+, reactive oxygen species (ROS) signal transduction, regulating antioxidant enzymes, SA, JA, abscisic acid (ABA) and indole-3-acetic acid (IAA) signaling pathways, and up-regulating flavonoid biosynthesis-related genes. This study demonstrated that lactic acid might play a role in inducing resistance to tobacco black shank disease; the mechanism by which lactic acid induces disease resistance includes direct antifungal activity and inducing the host to produce direct and primed defenses. In conclusion, this study provided a theoretical basis for lactic acid-induced resistance and a new perspective for preventing and treating tobacco black shank disease.

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“…In the early stage of black shank disease infection, P. nicotianae obtains nutrition from plant cells and develops a necrotrophic lifestyle due to the death of plant cells (Lee and Rose, 2010;Wang, 2022). The application of chemical compounds is one method for controlling black shank disease (Ji et al, 2014;Suohui et al, 2022). However, the chemicals are not only costly but also harmful to the environment.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomics and iTRAQ-proteomics analyses provide novel insights into the defense mechanism of black shank disease in tobacco

et al. 2022

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Black shank disease caused by Phytophthora nicotianae is one of the most important diseases in tobacco worldwide and can result in a devastating loss in tobacco cultivation. Many efforts have been carried out to identify the chromosome segment from Nicotiana plumbaginifolia containing a resistance locus carrying a gene named Php; however, the Php gene has not been cloned, and knowledge of the potential mechanism of the Php gene in the resistant lines is limited. To further characterize the resistance mechanism of the Php gene, we first used the resistant line “RBST” and the susceptible cultivar “Honghuadajinyuan” (HD) to obtain the near-isogenic line RBS89 containing the Php gene from RBST. RBS89 showed high resistance to black shank disease. Transcriptomic and iTRAQ analyses were applied to explore the potential defense mechanisms in RBS89 plants in comparison with HD plants with or without inoculation. Many differentially expressed genes (DEGs) and proteins were identified, and some pathogenesis-related (PR) proteins were extensively abundant in the RBS89 plants when compared with the HD plants in response to black shank disease. Importantly, overexpression of the PR gene NtPR-1B in HD plants improved the resistance of tobacco plants to black shank disease, indicating that NtPR-1B and Php genes might have similar roles in protecting tobacco from black shank disease. However, the relationship between NtPR-1B and Php genes requires further analysis. Therefore, our study provides valuable information for breeding tobacco cultivars with black shank disease resistance and sheds light on the defense mechanism of black shank disease in tobacco for enhancing Phytophthora resistance in other Solanaceae crops.

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Thiamine induces resistance in tobacco against black shank

Cited by 5 publications

References 53 publications

Cytological and transcriptomic analysis to unveil the mechanism of web blotch resistance in Peanut

Cytological and transcriptomic analysis to unveil the mechanism of web blotch resistance in Peanut

Lactic acid induced defense responses in tobacco against Phytophthora nicotianae

Transcriptomics and iTRAQ-proteomics analyses provide novel insights into the defense mechanism of black shank disease in tobacco

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