Thiamine increases β-glucosidase production in the newly isolated strain of<i>Fomitopsis pinicola</i>

Joo, Ah-Reum; Lee, K.-M.; Sim, Won-Il; Jeya, Marimuthu; Hong, Myoung-Ki; Kim, Y.-S.; Oh, Deok‐Kun; Lee, J.-K.

doi:10.1111/j.1472-765x.2009.02639.x

Cited by 5 publications

(2 citation statements)

References 35 publications

(42 reference statements)

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“…Additionally, vitamins are easy to deliver during liquid fermentation (Joo et al 2009). To examine the effect of vitamins on BGL production and to select a suitable vitamin concentration, thiamine HCl ranging from 0.01 to 0.2% (w/v) was added to medium containing 3% cellulose and 2% soy peptone.…”

Section: Effect Of Carbon Source Nitrogen Source and Vitaminmentioning

confidence: 99%

Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass

Lee¹,

Lee²,

Kim³

et al. 2014

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Medium components for β-glucosidase (BGL) production in Schizophyllum commune KUC9397 were optimized using a central composite design and response surface methodology. From the various medium components tested, cellulose, soy peptone, and thiamine HCl were selected as the optimal carbon, nitrogen, and vitamin sources, respectively. The highest BGL production was obtained with 2.96% cellulose, 2.30% soy peptone, and 0.11% thiamine HCl. BGL production in the optimized medium was increased 7.2-fold compared to production in an unoptimized medium. Crude enzyme preparation from S. commune KUC9397 was used to saccharify pretreated lignocellulosic biomass. The crude enzyme preparations showed statistically equal saccharification rates as Cellobiase, a commercial BGL. This finding indicates that crude enzymes produced by S. commune KUC9397 have good potential for application in cellulosic biomass conversion systems in place of Cellobiase.

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Section: Effect Of Carbon Source Nitrogen Source and Vitaminmentioning

confidence: 99%

Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass

Lee¹,

Lee²,

Kim³

et al. 2014

BioResources

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“…The micronutrients and thiamine in the nutritional media, induced the production of individual enzymes in fungal proteome, through increasing the expression of several proteins, However, the present results confirm that the supplementation of T. harzianum medium with the tested chemicals could improve and increase the efficacy of the overall biological control process (Joo et al, 2009). The positive effect of antioxidants improved the metabolic activity and can be used by the fungus as a nutrients or coenzyme.…”

Section: Resultsmentioning

confidence: 46%

Effect of some Chemical Inducers on Antagonistic Potential of Trichoderma harzianum against Rhizoctonia solani and it's Metabolites Production

Yousef

Salem

El-Sharkawy

2018

Journal of Plant Protection and Pathology

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The present study was conducted on different supplemented gliotoxin growth medium by using some chemical inducers (micronutrients mixture, potassium tartrate and thiamine) to improve antagonism, crude extracts and volatile compounds activities of T. harzianum against R. solani. T. harzianum grown on medium supplemented with potassium tartrate decreased the mycelial growth of R. solani by 89% compared with T. harzianum alone (63.3%). Also, exhibited the significantly higher activity of the crude extract and the volatile compounds. The micronutrients followed by the thiamine addition recorded the highest activity of the secondary metabolite. Treated Trichoderma enhanced bioactivity against the pathogen when transferred on PDA medium without addition of the tested materials. Light microscope observation revealed that malformed and damaged pathogen hyphae occurred more clearly after contact with the treat ed T. harzianum. The GC-MS analysis showed detection of antifungal compounds as 2,3-butanediol, Decane, 2,4,6-trimethyl-, phenylethyl alcohol, acetic acid,

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Optimization of β-Glucosidase Production by a Strain of Stereum hirsutum and Its Application in Enzymatic Saccharification

Priyadharshini

Nguyen²,

Choi³

et al. 2013

J. Microbiol. Biotechnol.

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A high beta-glucosidase (BGL)-producing strain, Stereum hirsutum, was identified and isolated and showed a maximum BGL activity (10.4 U/ml) when cultured with Avicel and tryptone as the carbon and nitrogen sources, respectively. In comparison with other BGLs, BGL obtained from S. hirsutum showed a higher level of activity to cellobiose (V(max) = 172 U/mg, and k(cat) = 281/s). Under the optimum conditions (600 rpm, 30°C, and pH 6.0), the maximum BGL activity of 10.4 U/ml with the overall productivity of 74.5 U/l/h was observed. BGL production was scaled up from a laboratory scale (7-L fermenter) to a pilot scale (70-L fermenter). When S. hirsutum was cultured in fed-batch culture with rice straw as the carbon source in a 70-L fermenter, a comparable productivity of 78.6 U/l/h was obtained. Furthermore, S. hirsutum showed high levels of activity of other lignocellulases (cellobiohydrolase, endoglucanase, xylanase, and laccase) that are involved in the saccharification of biomasses. Application of S. hirsutum lignocellulases in the hydrolysis of Pinus densiflora and Catalpa ovata showed saccharification yields of 49.7% and 43.0%, respectively, which were higher than the yield obtained using commercial enzymes.

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Thiamine increases β-glucosidase production in the newly isolated strain ofFomitopsis pinicola

Cited by 5 publications

References 35 publications

Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass

Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass

Effect of some Chemical Inducers on Antagonistic Potential of Trichoderma harzianum against Rhizoctonia solani and it's Metabolites Production

Optimization of β-Glucosidase Production by a Strain of Stereum hirsutum and Its Application in Enzymatic Saccharification

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