Thiamin deficiency induces impaired fish gill immune responses, tight junction protein expression and antioxidant capacity: Roles of the NF-κB, TOR, p38 MAPK and Nrf2 signaling molecules

Wen, Ling‐Mei; Feng, Lin; Jiang, Wei; Liu, Yang; Wu, Pei; Zhao, Juan; Jiang, Jun; Kuang, Sheng‐Yao; Tang, Ling; Tang, Wu‐Neng; Zhang, Yong-An; Zhou, Xiao‐Qiu

doi:10.1016/j.fsi.2015.12.038

Cited by 21 publications

(8 citation statements)

References 72 publications

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“…Lysine, one of the limiting amino acids, is also a signaling molecular that regulates the immune response ( 43 ). In mammals and fish, it was revealed that TOR could activate S6K1 and inhibit 4E-BPs, which then regulate anti-inflammatory cytokines ( 44 , 45 ). In our experiment, the relative mRNA expression levels of TOR and S6K1 were markedly upregulated when fish were fed a 2.44% lysine diet compared with a control diet (1.44% dietary lysine).…”

Section: Discussionmentioning

confidence: 99%

Dietary Lysine Levels Improved Antioxidant Capacity and Immunity via the TOR and p38 MAPK Signaling Pathways in Grass Carp, Ctenopharyngodon idellus Fry

et al. 2021

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An 8-week rearing trial was designed to appraise the dietary lysine levels on intestinal antioxidant capacity and immunity of grass carp fry. Six practical diets were prepared with graded levels of lysine (1.44, 1.79, 1.97, 2.44, 2.56 and 2.87% dry matter), and these diets were fed to grass carp fry. The results showed that the activities of intestinal antioxidant factors including catalase and glutathione peroxidase were markedly improved by the 2.44% dietary lysine compared with the control diet (1.44% dietary lysine) (P < 0.05). In terms of antioxidants, compared with the control diet, the 2.44% diet markedly upregulated the mRNA expression levels of target of rapamycin, S6 kinase1 and nuclear factor erythroid 2-related factor 2 pathway-related antioxidant genes, containing catalase and glutathione peroxidase 1α (P < 0.05) and downregulated the mRNA levels of Kelch-like ECH-associated protein 1 (P > 0.05). The mRNA levels of 4E-binding protein 2 showed the opposite trend compared with those of target of rapamycin, and the minimum value was observed in the group of 1.97% dietary lysine (P < 0.05). In terms of immunity, compared with the 1.44% diet, the 2.44% diet markedly suppressed the intestinal p38 mitogen-activated protein kinase and interferon γ2 mRNA levels (P < 0.05). Moreover, nuclear factor-kappa B p65, tumor necrosis factor α, interleukin 6, interleukin 8, and interleukin 15 mRNA levels all exhibited the same trend as p38 mitogen-activated protein kinase and interferon γ2; however, the difference among all the lysine treatments groups was not significant (P > 0.05). The anti-inflammatory cytokines transforming growth factor β2 and interleukin 4/13B mRNA levels in the intestine were remarkably upregulated by high dietary lysine levels (2.56 and 2.87%) (P < 0.05), and when the dietary lysine level reached 2.44%, the interleukin 4/13A mRNA levels were strikingly increased (P < 0.05). Overall, the data suggested that 2.44% dietary lysine could strengthen the immune and antioxidant capacities of grass carp fry via activating the target of rapamycin (TOR) signaling pathway, and suppressing the p38 mitogen-activated protein kinase (p38 MAPK) signaling pathway, which then improve the survival rate.

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Section: Discussionmentioning

confidence: 99%

Dietary Lysine Levels Improved Antioxidant Capacity and Immunity via the TOR and p38 MAPK Signaling Pathways in Grass Carp, Ctenopharyngodon idellus Fry

et al. 2021

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“…However, high PUFAs content is highly susceptible to oxidative damage, leading to decrease in fish flesh quality (Wang & Shahidi, 2017; Zhang et al, 2015). Moreover, our published study indicated that thiamin deficiency impaired the antioxidative ability in on‐growing grass carp intestine and gill (Wen et al, 2015, 2016). Thus, we hypothesize that an appropriate level of thiamin is required in fish for improving muscle quality, which warrants investigation.…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary thiamin on flesh quality in grass carp ( Ctenopharyngodon idella )

Wen

Jiang

et al. 2022

Aquaculture Research

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Thiamin, an essential water-soluble vitamin for fish, functions as cofactor for enzymes that are crucial in pentose phosphate pathway (National Research Council (NRC), 2011), which is pivotal regarding the enforcement of the antioxidant system (Oruch & Pryme, 2020). Fish muscle, the main edible portion for consumers, is abundant of polyunsaturated fatty acids (PUFAs). However, high PUFAs content is highly susceptible to oxidative damage, leading to decrease in fish flesh quality (Wang & Shahidi, 2017;Zhang et al., 2015). Moreover, our published study indicated that thiamin deficiency impaired the antioxidative ability in on-growing grass carp intestine and gill (Wen

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“…Dysfunction of gill osmoregulation can cause fish to become unable to adapt to changes in environmental salinity, and thus result in death. Therefore, adaptive regulation to maintain gill osmoregulatory function is critically important for fish living in variable aquatic environments [ 57 ]. In the present study, the differential expression profiles of the gill proteome in response to hypo- and hypersaline stress in S. argus were elucidated.…”

Section: Discussionmentioning

confidence: 99%

Osmoregulatory strategies of estuarine fish Scatophagus argus in response to environmental salinity changes

Liu

Zhang

et al. 2022

BMC Genomics

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Background Scatophagus argus, an estuarine inhabitant, can rapidly adapt to different salinity environments. However, the knowledge of the molecular mechanisms underlying its strong salinity tolerance remains unclear. The gill, as the main osmoregulatory organ, plays a vital role in the salinity adaptation of the fish, and thus relative studies are constructive to reveal unique osmoregulatory mechanisms in S. argus. Results In the present study, iTRAQ coupled with nanoLC-MS/MS techniques were employed to explore branchial osmoregulatory mechanisms in S. argus acclimated to different salinities. Among 1,604 identified proteins, 796 differentially expressed proteins (DEPs) were detected. To further assess osmoregulatory strategies in the gills under different salinities, DEPs related to osmoregulatory (22), non-directional (18), hypo- (52), and hypersaline (40) stress responses were selected. Functional annotation analysis of these selected DEPs indicated that the cellular ion regulation (e.g. Na+-K+-ATPase [NKA] and Na+-K+-2Cl− cotransporter 1 [NKCC1]) and ATP synthesis were deeply involved in the osmoregulatory process. As an osmoregulatory protein, NKCC1 expression was inhibited under hyposaline stress but showed the opposite trend in hypersaline conditions. The expression levels of NKA α1 and β1 were only increased under hypersaline challenge. However, hyposaline treatments could enhance branchial NKA activity, which was inhibited under hypersaline environments, and correspondingly, reduced ATP content was observed in gill tissues exposed to hyposaline conditions, while its contents were increased in hypersaline groups. In vitro experiments indicated that Na+, K+, and Cl− ions were pumped out of branchial cells under hypoosmotic stress, whereas they were absorbed into cells under hyperosmotic conditions. Based on our results, we speculated that NKCC1-mediated Na+ influx was inhibited, and proper Na+ efflux was maintained by improving NKA activity under hyposaline stress, promoting the rapid adaptation of branchial cells to the hyposaline condition. Meanwhile, branchial cells prevented excessive loss of ions by increasing NKA internalization and reducing ATP synthesis. In contrast, excess ions in cells exposed to the hyperosmotic medium were excreted with sufficient energy supply, and reduced NKA activity and enhanced NKCC1-mediated Na+ influx were considered a compensatory regulation. Conclusions S. argus exhibited divergent osmoregulatory strategies in the gills when encountering hypoosmotic and hyperosmotic stresses, facilitating effective adaptabilities to a wide range of environmental salinity fluctuation.

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Thiamin deficiency induces impaired fish gill immune responses, tight junction protein expression and antioxidant capacity: Roles of the NF-κB, TOR, p38 MAPK and Nrf2 signaling molecules

Cited by 21 publications

References 72 publications

Dietary Lysine Levels Improved Antioxidant Capacity and Immunity via the TOR and p38 MAPK Signaling Pathways in Grass Carp, Ctenopharyngodon idellus Fry

Dietary Lysine Levels Improved Antioxidant Capacity and Immunity via the TOR and p38 MAPK Signaling Pathways in Grass Carp, Ctenopharyngodon idellus Fry

Effects of dietary thiamin on flesh quality in grass carp ( Ctenopharyngodon idella )

Osmoregulatory strategies of estuarine fish Scatophagus argus in response to environmental salinity changes

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