Theta Temperatures

Sundararajan, P. R.

doi:10.1007/978-0-387-69002-5_15

Cited by 4 publications

(2 citation statements)

References 303 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The temperature where the expansion stops is referred to as the θ condition, where hydrogel insolubility cancels the effects of excluded volume. 44 In the presence of low numbers of supramolecular protein cross-links (triangles in Figure 6), thermal swelling is counterbalanced, and therefore lower than that of blank hydrogel, by a decrease in volume exclusion occurring from restricted polymer chains. In addition, the θ temperature increased, indicating that the restricted polymer chains require more thermal energy (versus blank hydrogel) to be surrounded by solvent as expected for volume exclusion effects.…”

Section: Scheme 2 Aptamer Design and Preparation Steps For Pdgf-ββ Su...mentioning

confidence: 99%

Macromolecular Amplification of Binding Response in Superaptamer Hydrogels

Bai¹,

Gariano²,

Spivak³

2013

J. Am. Chem. Soc.

118

View full text Add to dashboard Cite

It is becoming more important to detect ultralow concentrations of analytes for biomedical, environmental, and national security applications. Equally important is that new methods should be easy to use, inexpensive, portable, and if possible allow detection by the naked eye. By and large, detection of low concentrations of analytes cannot be achieved directly but requires signal amplification by catalysts, macromolecules, metal surfaces, or supramolecular aggregates. The rapidly progressing field of macromolecular signal amplification has been advanced using conjugated polymers, chirality in polymers, solvating polymers, and polymerization/depolymerization strategies. A new type of aptamer-based hydrogel with specific response to target proteins presented in this report demonstrates an additional category of macromolecular signal amplification. This superaptamer assembly provides the first example of using protein-specific aptamers to create volume-changing hydrogels with amplified response to the target protein. A remarkable aspect of these superaptamer hydrogels is that volume shrinking is visible to the naked eye down to femtomolar concentrations of protein. This extraordinary macromolecular amplification is attributed to a complex interplay between protein-aptamer supramolecular cross-links and the consequential reduction of excluded volume in the hydrogel. Specific recognition is even maintained in biological matrices such as urine and tears. Furthermore, the gels can be dried for long-term storage and regenerated for use without loss of activity. In practice, the ease of this biomarker detection method offers an alternative to traditional analytical techniques that require sophisticated instrumentation and highly trained personnel.

show abstract

Section: Scheme 2 Aptamer Design and Preparation Steps For Pdgf-ββ Su...mentioning

confidence: 99%

Macromolecular Amplification of Binding Response in Superaptamer Hydrogels

Bai¹,

Gariano²,

Spivak³

2013

J. Am. Chem. Soc.

118

View full text Add to dashboard Cite

show abstract

“…To produce particles with higher porosities, we mixed a thermodynamically good solvent (toluene) with ACN to favor solvation of the incoming polymeric phase . Li and Stover reported the effectiveness of such mixture for precipitation polymerizations when DVB was used as a cross-linker, as it yielded high-quality bead-shaped polymers with reasonable yields and well developed pore structure .…”

Section: Resultsmentioning

confidence: 99%

Experimental Mixture Design as a Tool for the Synthesis of Antimicrobial Selective Molecularly Imprinted Monodisperse Microbeads

Benito‐Peña

Navarro-Villoslada

Carrasco

et al. 2015

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

The effect of the cross-linker on the shape and size of molecular imprinted polymer (MIP) beads prepared by precipitation polymerization has been evaluated using a chemometric approach. Molecularly imprinted microspheres for the selective recognition of fluoroquinolone antimicrobials were prepared in a one-step precipitation polymerization procedure using enrofloxacin (ENR) as the template molecule, methacrylic acid as functional monomer, 2-hydroxyethyl methacrylate as hydrophilic comonomer, and acetonitrile as the porogen. The type and amount of cross-linker, namely ethylene glycol dimethacrylate, divinylbenzene or trimethylolpropane trimethacrylate, to obtain monodispersed MIP spherical beads in the micrometer range was optimized using a simplex lattice design. Particle size and morphology were assessed by scanning electron microscopy, dynamic light scattering, and nitrogen adsorption measurements. Electron paramagnetic resonance spectroscopy in conjunction with a nitroxide as spin probe revealed information about the microviscosity and polarity of the binding sites in imprinted and nonimprinted polymer beads.

show abstract

Introduction

2016

Physical Aspects of Polymer Self‐Assembly

View full text Add to dashboard Cite

Theta Temperatures

Cited by 4 publications

References 303 publications

Macromolecular Amplification of Binding Response in Superaptamer Hydrogels

Macromolecular Amplification of Binding Response in Superaptamer Hydrogels

Experimental Mixture Design as a Tool for the Synthesis of Antimicrobial Selective Molecularly Imprinted Monodisperse Microbeads

Introduction

Contact Info

Product

Resources

About