Thermotropic Phase Behavior of Hydrogenated Soybean Phosphatidylcholine–Cholesterol Binary Liposome Membrane

Kitayama, H.; Takechi, Yoshizumi; Tamai, Naoto; Matsuki, Hitoshi; Yomota, Chikako; Saito, Hiroyuki

doi:10.1248/cpb.c13-00587

Cited by 44 publications

(30 citation statements)

References 24 publications

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“…Neither spontaneous leakage nor thermal effects can occur up to 50 °C. Our results are in agreement with those reported by Kitayama and co-workers in [ 23 ].…”

Section: Resultssupporting

confidence: 94%

Can Pulsed Electromagnetic Fields Trigger On-Demand Drug Release from High-Tm Magnetoliposomes?

et al. 2018

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Recently, magnetic nanoparticles (MNPs) have been used to trigger drug release from magnetoliposomes through a magneto-nanomechanical approach, where the mechanical actuation of the MNPs is used to enhance the membrane permeability. This result can be effectively achieved with low intensity non-thermal alternating magnetic field (AMF), which, however, found rare clinic application. Therefore, a different modality of generating non-thermal magnetic fields has now been investigated. Specifically, the ability of the intermittent signals generated by non-thermal pulsed electromagnetic fields (PEMFS) were used to verify if, once applied to high-transition temperature magnetoliposomes (high-Tm MLs), they could be able to efficiently trigger the release of a hydrophilic model drug. To this end, hydrophilic MNPs were combined with hydrogenated soybean phosphatidylcholine and cholesterol to design high-Tm MLs. The release of a dye was evaluated under the effect of PEMFs for different times. The MNPs motions produced by PEMF could effectively increase the bilayer permeability, without affecting the liposomes integrity and resulted in nearly 20% of release after 3 h exposure. Therefore, the current contribution provides an exciting proof-of-concept for the ability of PEMFS to trigger drug release, considering that PEMFS find already application in therapy due to their anti-inflammatory effects.

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“…Neither spontaneous leakage nor thermal effects can occur up to 50 °C. Our results are in agreement with those reported by Kitayama and co-workers in [ 23 ].…”

Section: Resultssupporting

confidence: 94%

Can Pulsed Electromagnetic Fields Trigger On-Demand Drug Release from High-Tm Magnetoliposomes?

et al. 2018

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“…2 due to the addition of cholesterol is a decrease in T m values when the DSPC membrane contains up to 33 mol% of cholesterol. This behavior is in agreement with literature data performed on liposome membranes made of DNPC 57 (di24:1c15 PC, 1,2-dinervonoyl-sn-glycero-3-phosphocholine), HSPC 58 (Hydrogenated soybean phosphatidylcholine made approximately of 85% DSPC and 15% DPPC), di17:0 PC 59 (1,2-diheptadecanoyl-sn-glycero-3-phosphocholine), DPPC, 11,52,60 and DOPC. 61 This effect can be explained by the fact that the addition of cholesterol rearranges the membrane structure: both L α and L β phases are progressively disappearing upon the addition of cholesterol to be progressively replaced by a homogeneous liquid ordered or L o phase.…”

Section: Discussionsupporting

confidence: 92%

Molecular Study of Ultrasound-Triggered Release of Fluorescein from Liposomes

et al. 2021

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Several investigations have suggested that ultrasound triggers the release of drugs encapsulated into liposomes at acoustic pressures low enough to avoid cavitation or high hyperthermia. However, the mechanism leading to this triggered release as well as the adequate composition of the liposome membrane remain unknown. Here, we investigate the ultrasound triggered release of fluorescein disodium salt encapsulated into liposomes made of 1,2-dioleoyl-sn-glycero-3-phosphocholi (DOPC) or 1,2-distearoylphosphatidylethanolamie (DSPC) lipids with various concentrations of cholesterol (from 0 to 44 mol%).The passive release of encapsulated fluorescein was first characterized. It was observed to be higher when the membrane is in a fluid phase and increased with temperature but decreased upon addition of cholesterol. Next, the release of fluorescein was measured at 1 different acoustic frequencies (0.8, 1.1, 3.3 MHz) and peak-to-peak pressures (0, 2, 2.5, 5, 8 MPa). Measurements were performed at temperatures where DOPC and DSPC liposomes were respectively in the fluid or gel phase. We found that the release rate did not depend on the ultrasound frequency. For DOPC liposomes, the ultrasoundtriggered release of fluorescein decreased with increasing concentration of cholesterol in liposomes, while the behavior was more complex for DSPC liposomes. Overall, the triggered release from DSPC liposomes was up to ten times less than DOPC liposomes.Molecular dynamics simulations performed on a pure DOPC membrane showed that a membrane experiences, under a directional pressure of ±2.4 MPa, various changes in properties such as the area per lipid (APL). An increase in APL was notably observed when the simulation box was laterally stretched or perpendicularly compressed, which was accompanied by an increase in the number of water molecules crossing the membrane. This suggests that ultrasound most probably enhances the diffusion of encapsulated molecules at small acoustic pressures by increasing the distance between lipids.

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“…Furthermore, Naziris et al [123] prepared pH-responsive LPs containing structurally different chimeric LP systems made by HSPC (which exhibit phase transition temperature from gel phase (Lβ) to liquid crystalline phase (Lα) [124]) and poly(2dimethylamino)ethyl methacrylate)-beta-poly(lauryl methacrylate) (PDMAEMA-beta-PLMA, 53, Fig. 12) by thin-film hydration methods.…”

Section: Ph-sensitive Polymers and Copolymersmentioning

confidence: 99%

Stimulus-responsive liposomes for biomedical applications

Antoniou

Giofrè

Seneci

et al. 2021

Drug Discovery Today

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Liposomes are amphipathic lipidic supramolecular aggregates able to encapsulate and carry molecules of both hydrophilic and hydrophobic nature. They have been widely used as in vivo drug delivery systems since long due to their favorable features, such as synthetic flexibility, biodegradability, biocompatibility, low immunogenicity, and negligible toxicity. In recent years, the possibility to introduce chemical modifications on liposomes has paved the way to smart liposome-based drug delivery systems characterized by even more tunable and disease-directed features. In this review, the different types of chemical modifications introduced so far have been highlighted, with a particular focus on internal stimuli-responsive liposomes and pro-drug activation.

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Thermotropic Phase Behavior of Hydrogenated Soybean Phosphatidylcholine–Cholesterol Binary Liposome Membrane

Cited by 44 publications

References 24 publications

Can Pulsed Electromagnetic Fields Trigger On-Demand Drug Release from High-Tm Magnetoliposomes?

Can Pulsed Electromagnetic Fields Trigger On-Demand Drug Release from High-Tm Magnetoliposomes?

Molecular Study of Ultrasound-Triggered Release of Fluorescein from Liposomes

Stimulus-responsive liposomes for biomedical applications

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