Thermotropic behavior of lipids and the morphology of Yersinia pseudotuberculosis cells with a high content of lysophosphatidylethanolamine

Бахолдина, С. И.; Sanina, N. M.; Shubin, F. N.; Popova, Olga; Соловьева, Т. Ф.

doi:10.1134/s0026261707030046

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…For example, bacterial growth is subject to abiotic stresses, such as hydrostatic pressure that changes the storage lipid composition (Grossi et al 2010). Temperature-related changes in membrane PLs have been reported (Bakholdina et al 2007). Bacterial growth temperature has a direct influence on membrane protein expression, and membrane proteins can be upregulated or downregulated with temperature, which in turn, affects lipid metabolism and the constituent FAs.…”

Section: Effect Of Temperature On Fa Ratiomentioning

confidence: 97%

A comparative study of fatty acid profile and formation of biofilm inGeobacillus gargensisexposed to variable abiotic stress

et al. 2015

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Understanding bacterial fatty acid (FA) profile has a great taxonomic significance as well as clinical importance for diagnosis issues. Both the composition and nature of membrane FAs change under different nutritional, biotic and (or) abiotic stresses, and environmental stress. Bacteria produce both odd-carbon as well as branched-chain fatty acids (BCFAs). This study was designed to examine the effect of abiotic pressure, including salinity, temperature, pH, and oxinic stress on the growth, development, and FA profile in thermophilic Geobacillus gargensis. Under these stresses, 3 parametric ratios, 2-methyl fatty acids/3-methyl fatty acids (iso-/anteiso-FAs), BCFAs/straight-chain saturated fatty acids (SCSFA), and SCSFAs/straight-chain unsaturated fatty acids (SCUFA), in addition to total lipids affected by variable stresses were measured. Our results indicate that the ratio of total iso-/anteiso-FAs increased at the acidic pH range of 4.1-5.2 and decreased with increasing pH. The reverse was true for salt stress when iso-/anteiso-FAs ratio increased with salt concentration. The BCFAs/SCSFAs and SCSFAs/SCUFAs ratios increased at neutral and alkaline pH and high salt concentration, reduced incubation time, and comparatively high temperature (55-65 °C) of the growth medium. The bacterial total lipid percentage deceased with increasing salt concentration, incubation period, but it increased with temperature. The formation of extracellular polymeric substances was observed under all stress conditions and with the addition of sodium dodecyl sulfate (2 and 5 mmol/L) to the growth medium. The membrane phospholipid composition of the bacterium was analyzed by thin-layer chromatography.

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Section: Effect Of Temperature On Fa Ratiomentioning

confidence: 97%

A comparative study of fatty acid profile and formation of biofilm inGeobacillus gargensisexposed to variable abiotic stress

et al. 2015

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“…Л.А. Тарасевича (в настоящее время -ФГБУ «Научный центр экспертизы средств медицинского применения» Минздрава России) и инкубировали в течение 24 ч. Работу проводили в соответствии с требованиями биологической безопасности 1 . Оптическую плотность обеззараженных культур определяли на спектрофотометре фирмы Beckman (США) при 600 нм.…”

Section: материалы и методыunclassified

Influence of temperature stress on the spectrum of fatty acids of Vibrio cholerae strains

Shipko¹,

Дуванова²

2022

Вест. ПГУ. Биол.

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Under conditions of simulated temperature stress gas chromatography combined with mass spectrometry was used to study the spectrum of fatty acids of strains of Vibrio cholerae O1, O139, non O1/non O139 serogroups with a different set of pathogenicity determinants isolated from patients and from water samples of surface water bodies. Significant differences in the composition of fatty acids under cold and heat stress in the cells of the causative agent of cholera relative to control samples were revealed. The strains isolated from the water of surface reservoirs differed in the spectrum of fatty acids under cold stress from the strains isolated from patients. In strains isolated from water, a decrease in cultivation temperature to 23 °C was accompanied by the synthesis of docosanoic, tetracosanoic and hexacosanoic acids and to 4 °C - an increase in the amount of tetradecanoic and hexadecanoic acids. While in strains isolated from patients, a decrease in cultivation temperature caused a regular increase in hexadecenoic acids. and octadecenoic acids. The reaction to heat stress in most of the studied strains regardless of the set of pathogenicity determinants and the source of isolation had a general trend: an increase in the total amount of saturated fatty acids, the synthesis of trans-isomers of unsaturated fatty acids and the appearance of ω-alicyclic and iso-branched fatty acids. In addition to the remodeling of the fatty acid composition of the membrane under the influence of temperature stress, the synthesis of oxylipins, phenylpropanoids and terpenoids, possibly playing the role of adaptogens, was noted.

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“…Hypoxic environment of the intestine is critical for nutrient absorption, intestinal barrier function, and innate and adaptive immune responses in the intestine (Singhal and Shah, 2020). The oxygen deficiency during growth promotes increasing of pathogenic potential of Y. pseudotuberculosis (Bakholdina et al, 2009), the outcomes of artificial oxidative stress arouse our curiosity.…”

Section: Introductionmentioning

confidence: 99%

The Assessment on Synergistic Activity of Ebselen and Silver Ion Against Yersinia pseudotuberculosis

et al. 2022

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Yersinia pseudotuberculosis is a foodborne zoonotic bacterium that is pathogenic to guinea pigs, rabbits, and mice. It also causes pseudotuberculosis in humans. However, it still lacked the scientific basis for control. Here, we found out that Ebselen (EbSe) exhibited synergistic antibacterial activity with silver nitrate (Ag+) against Y. pseudotuberculosis YpIII strain with high efficacy in vitro using UV-visible light absorption spectrum, 5,5’-dithiobis-(2-nitrobenzoic acid), laser scanning confocal microscope, flow cytometry, transmission electron microscopy and Western blotting assays. The depletion of total glutathione (GSH) amount and inhibition of thioredoxin reductase (TrxR) activity in thiol-dependent redox system revealed the destructiveness of EbSe-Ag+-caused intracellular oxidative stress. Furthermore, a YpIII-caused mice gastroenteritis model was constructed. EbSe-Ag+ significantly reduced bacterial loads with low toxicity. It also down-regulated the expression levels of interferon (IL)-1β and tumor necrosis factor-α, up-regulated the expression level of IL-10 on-site. All the in vivo results demonstrated the antibacterial activity and immune-modulatory property of EbSe-Ag+. Collectively, these results provided academic fundament for further analysis and development of EbSe-Ag+ as the antibacterial agents for pseudotuberculosis control.

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Thermotropic behavior of lipids and the morphology of Yersinia pseudotuberculosis cells with a high content of lysophosphatidylethanolamine

Cited by 3 publications

References 16 publications

A comparative study of fatty acid profile and formation of biofilm inGeobacillus gargensisexposed to variable abiotic stress

A comparative study of fatty acid profile and formation of biofilm inGeobacillus gargensisexposed to variable abiotic stress

Influence of temperature stress on the spectrum of fatty acids of Vibrio cholerae strains

The Assessment on Synergistic Activity of Ebselen and Silver Ion Against Yersinia pseudotuberculosis

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