Thermotolerant lipase from Penicillium sp. section Gracilenta CBMAI 1583: Effect of carbon sources on enzyme production, biochemical properties of crude and purified enzyme and substrate specificity

Turati, Daniela Flavia Machado; Almeida, Alex Fernando de; Terrone, Cárol Cabral; Nascimento, Juliana M.F.; Terrasan, César Rafael Fanchini; Fernández‐Lorente, Gloria; Pessela, Benevides C.; Guisán, José M.; Carmona, Eleonora Cano

doi:10.1016/j.bcab.2018.10.002

Cited by 21 publications

(8 citation statements)

References 41 publications

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“…Effect of substrate concentration in the culture medium, on lipolytic activity was also studied by Turati et al [2019]. They cultivated the fungi called Penicillium sp.…”

Section: Resultsmentioning

confidence: 99%

Effect of Avocado Oil on The Induction of Yeast Hydrolases

Małajowicz

Rochalska

Wacławek

2020

Zeszyty Problemowe Postępów Nauk Rolniczych

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“…Effect of substrate concentration in the culture medium, on lipolytic activity was also studied by Turati et al [2019]. They cultivated the fungi called Penicillium sp.…”

Section: Resultsmentioning

confidence: 99%

Effect of Avocado Oil on The Induction of Yeast Hydrolases

Małajowicz

Rochalska

Wacławek

2020

Zeszyty Problemowe Postępów Nauk Rolniczych

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“…Similarly, other lipases from Penicillium spp. showed significant instability in polar solvents [ 18 , 65 ]. The significant deactivation of enzymes in polar solvents may be due to the stripping-off of the essential bound water monolayer from the enzyme molecule [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

Purification, Biochemical and Kinetic Characterization of a Novel Alkaline sn-1,3-Regioselective Triacylglycerol Lipase from Penicilliumcrustosum Thom Strain P22 Isolated from Moroccan Olive Mill Wastewater

Hasnaoui

Dab

Mechri

et al. 2022

IJMS

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A novel extracellular lipase from a filamentous fungus Ascomycota strain, P22, was isolated from olive mill wastewater, then purified and characterized. This strain was identified as Penicillium crustosum Thom based on sequencing analyses. Penicillium crustosum Thom strain P22 lipase (PCrL) was purified 63-fold to homogeneity using ammonium sulfate precipitation and chromatography on a Q-Sepharose Fast Flow column, with a total yield of 34%. The purified PCrL had a molecular mass of 28 kDa, estimated by SDS-PAGE. The 20 NH2-terminal amino-acid residues showed a high degree of homology with those of other Penicillium lipases. The specific activity of PCrL at pH 9 and 37 °C were found to be 5000 and 10,000 U/mg on olive oil and trioctanoin emulsions, respectively. PCrL exhibited clear regioselectivity toward the sn-1 position of the surface-coated triglycerides which were esterified with α-eleostearic acid at the sn-1/3 position. PCrL was completely inhibited by 53 µM of Orlistat, 5 mM of phenylmethylsulfonylfluoride, and 2 mM of diiodopropyl fluorophosphate, suggesting that it belonged to the serine lipase family. PCrL showed high activity and stability in the presence of water-immiscible organic solvents, surfactant, and oxidizing agents, and showed considerable compatibility with commercial laundry detergents. Washing performance analysis revealed that it could effectively remove oil stains. Hence, PCrL has several attractive properties that make it a promising potential candidate for detergent formulations.

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“…On the other hand, acidic pH stimulates the production of fungal lipases. Turati et al (2019) recorded the enhanced output and activity of fungal lipase when the pH of the reaction process was kept at 4.0. Mahmoud et al (2015) investigated the effect of different pH ranges from 2.0 to 12 on the production of lipase .…”

Section: The Ph Of the Mediummentioning

confidence: 99%

Industrial applications of fungal lipases: a review

Kumar¹,

Verma²,

Dubey³

et al. 2023

Front. Microbiol.

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Fungal lipases (triacylglycerol acyl hydrolases EC 3.1.1.3) are significant industrial enzymes and have several applications in a number of industries and fields. Fungal lipases are found in several species of fungi and yeast. These enzymes are carboxylic acid esterases, categorized under the serine hydrolase family, and do not require any cofactor during the catalyzing of the reactions. It was also noticed that processes including the extraction and purification of lipases from fungi are comparatively easier and cheaper than other sources of lipases. In addition, fungal lipases have been classified into three chief classes, namely, GX, GGGX, and Y. Fungal lipases have applications not only in the hydrolysis of fats and oils (triglycerides) but are also involved in synthetic reactions such as esterification, acidolysis, alcoholysis, interesterification, and aminolysis. The production and activity of fungal lipases are highly affected by the carbon source, nitrogen source, temperature, pH, metal ions, surfactants, and moisture content. Therefore, fungal lipases have several industrial and biotechnological applications in many fields such as biodiesel production, ester synthesis, production of biodegradable biopolymers, formulations of cosmetics and personal care products, detergent manufacturing, degreasing of leather, pulp and paper production, textile industry, biosensor development, and drug formulations and as a diagnostic tool in the medical sector, biodegradation of esters, and bioremediation of wastewater. The immobilization of fungal lipases onto different carriers also helps in improving the catalytic activities and efficiencies of lipases by increasing thermal and ionic stability (in organic solvents, high pH, and temperature), being easy to recycle, and inducing the volume-specific loading of the enzyme onto the support, and thus, these features have proved to be appropriate for use as biocatalysts in different sectors.

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Thermotolerant lipase from Penicillium sp. section Gracilenta CBMAI 1583: Effect of carbon sources on enzyme production, biochemical properties of crude and purified enzyme and substrate specificity

Cited by 21 publications

References 41 publications

Effect of Avocado Oil on The Induction of Yeast Hydrolases

Effect of Avocado Oil on The Induction of Yeast Hydrolases

Purification, Biochemical and Kinetic Characterization of a Novel Alkaline sn-1,3-Regioselective Triacylglycerol Lipase from Penicilliumcrustosum Thom Strain P22 Isolated from Moroccan Olive Mill Wastewater

Industrial applications of fungal lipases: a review

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