Thermotolerant Extracellular Proteases Produced by Bacillus subtilis Isolated from Local Soil that Representing Industrial Applications

Uddin, Md. Sharif; Ahmad, Tanvir; Ajam, MM; Moniruzzaman, Mohammad; Mandol, D; Ray, S. C.; Sufian, Abu; Rahman, M.; Hossain, Emon; Ahammed, R

doi:10.22207/jpam.11.2.12

Cited by 34 publications

(4 citation statements)

References 18 publications

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“…However, in other studies in Ethiopia, predominant bacterial isolates were Proteus spp., followed by Staphylococcus aureus (Abera et al, 2011). Probable explanation to this difference in isolated bacterial species is due to the effect of climate and geographical variation among the countries (Uddin et al, 2017). The other organisms which have been isolated in our study were Streptococcus spp., Proteus spp., Klebsiella spp.…”

Section: Discussionmentioning

confidence: 51%

Enumeration of the Antimicrobial Susceptibility Patterns of Different Bacterial Isolates from ENT Patients with Ear Infections

2020

EJMHS

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Children are more susceptible to ear infection than adults worldwide, especially in developing countries. In Bangladesh like other developing countries antibiotics is a common choice of treatment in an ear infection. This study was sought to determine causative agents both in right and left ear infection and their antibacterial susceptibility pattern from patients with an ear infection. Specimens of ear discharge were collected aseptically using a sterile swab and cultured on MacConkey’s agar, blood agar, and chocolate agar. After inoculating on appropriate culture media bacterial isolates were identified by their colony morphology, culture characteristics, Gram reaction, and biochemical tests. In this study, a total of 70 positive cases of patients were included. Among these 27 (38.6%) were male, and 43(61.4%) were female. The prevalence of ear infection was significantly high in females (61.4%) than males (38.6%). The predominant bacterial isolates from positive culture were Staphylococcus aureus 43(61.4%), followed by Pseudomonas spp., 13 (18.6%), Streptococcus spp., 8(11.4%), Proteus spp., 5(7.2%), and Klebsiella spp., 1(1.4%). The antibacterial agent like Amikacin and Gentamicin showed a high level of antibacterial effect on all identified bacterial isolates. On the other hand, 98.6% of isolates were found highly resistant to Co-Trimoxazole and Flucloxacillin. Moreover, Streptococcus spp., Proteus spp., Klebsiella spp., and Pseudomonas spp., were highly resistant to multiple drugs (more than 4). A high degree of antibiotic resistance was observed among most of the drugs used in this study. However, Amikacin and Gentamicin were highly effective against the isolated bacterial species. Therefore, culture and susceptibility tests are vital for the appropriate treatment of ear infection.

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Section: Discussionmentioning

confidence: 51%

Enumeration of the Antimicrobial Susceptibility Patterns of Different Bacterial Isolates from ENT Patients with Ear Infections

2020

EJMHS

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“…However, microbial proteases are among the most important hydrolytic enzymes with great potential for use in various biotechnological processes, as food processing, pharmaceutical products and several other industries (Uddin et al, 2017). Caseinase is used in the food industry as a coagulant in cheese manufacturing and in the reduction of milk allergens, as well as in the energy drinks, dietary foods and health care industry (Dalmaso et al, 2015;Bhaturiwala et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

Identification, Characterization Enzymatic Antagonist Activity and Antimicrobial Resistance Profile of Bacteria Isolated From Brazilian Thermal Aquifer / Identificação, Caracterização Enzimática, Atividade Antagonista E Perfil De Resistência a Antimicrobianos De Bactérias Termotolerantes Isoladas Em Aquífero Termal Brasileiro

Xavier

Tambellini³

et al. 2021

BJD

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RESUMOO objetivo deste trabalho foi isolar identificar, rastrear enzimas de interesse biotecnológico, traçar o perfil de resistência a antimicrobianos e verificar a atividade antagonista de microrganismos termotolerantes isolados em aquífero termal em Caldas Novas, Brasil. Para tal foram coletadas amostras de águas profundas em três poços artesianos, inoculadas em ágar nutriente e incubadas a 37°C durante 24 horas. Os isolados foram identificados ao nível de espécie por espectrometria de massa MALDI-TOF. As espécies identificadas foram submetidas ao screening enzimático para amilase, celulase, gelatinase, caseinase, lipase e pectinase conforme metodologias padrão. O perfil de susceptibilidade das bactérias aos antimicrobianos foi realizado por disco difusão e a atividade antagonista foi avaliada frente à Escherichia coli ATCC 8739 e Staphilococcus aureus ATCC 25923. Foram obtidas quatro isolados identificados como Bacillus pumilus, Bacillus subtilis e dois Bacillus megaterium. Os isolados produziram caseinase e gelatinase e foram sensíveis a todos os antimicrobianos testados. Bacillus pumilus, Bacillus subtilis e B. megaterium 3207 exibiram atividade antagonista frente ao S. aureus. A atividade antagonista frente à cepa de S. aureus aqui encontrada abre uma perspectiva para futuros estudos de identificação de metabólitos secundários com potencial atividade antimicrobiana

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“…Bacterial proteases are among the most important hydrolytic enzymes with great potential for use in various biotechnological processes such as food processing, pharmaceutical products, and waste degradation. Several other industries (Uddin et al 2017) are increasing rapidly due to reduced processing time, low energy input, cost-effectiveness, and nontoxic and eco-friendly characteristics (Singh et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Determination of biotechnological potential of poultry litter isolated bacteria

Battisti,

Junkerfuerbom,

Machado

et al. 2024

Biosci. J.

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Poultry litter is composed of residues from industrial wood processing, serving as a bed for the animals. It has also been widely used as an agricultural biofertilizer, increasing its economic importance. Few studies report the microbiological composition of this material or its biotechnological potential, which was the objective of this study. The microbiological composition was obtained by 16sRNA genetic characterization, as well as, phosphate solubilization capacity, indole-3-acetic acid (IAA) production, fungal antagonism, and enzymatic capacity. As a result, 67 isolates were obtained, of which 30 were taxonomically positioned and 27 were biochemically characterized. The microbiota genera consisted of Staphylococcus (17%), Brevibacillus (27%), Bacillus (37%), Mammaliicoccus (7%), Isoptericola, Microbacterium, and Oxalicibacterium (3.5% each). None of the isolates presented phosphate solubilization capacity. Regarding IAA production, UFPRCA19 showed the highest IAA content (16.60 µg.mg-1 protein). Eleven isolates were positive for antagonism with Aspergillus spp. and 25 for Trichoderma spp. Activity of proteases, caseinases, lipases, amylases, and cellulases were observed with the relative frequencies of 59%, 29%, 11%, 32%, 18%, and 21%. IAA and enzyme-producing bacteria as well as fungal antagonism were found among the isolates. Among all the isolated genera, Bacillus for synthesizing IAA and commercial enzymes becomes an interesting source for biotechnological purposes.

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Thermotolerant Extracellular Proteases Produced by Bacillus subtilis Isolated from Local Soil that Representing Industrial Applications

Cited by 34 publications

References 18 publications

Enumeration of the Antimicrobial Susceptibility Patterns of Different Bacterial Isolates from ENT Patients with Ear Infections

Enumeration of the Antimicrobial Susceptibility Patterns of Different Bacterial Isolates from ENT Patients with Ear Infections

Determination of biotechnological potential of poultry litter isolated bacteria

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