Thermotoga lettingae sp. nov., a novel thermophilic, methanol-degrading bacterium isolated from a thermophilic anaerobic reactor.

Balk, Melike; Weijma, Jan; Stams, Alfons Johannes Maria

doi:10.1099/00207713-52-4-1361

Cited by 75 publications

(58 citation statements)

References 45 publications

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“…Very few SAOB have been successfully isolated and characterized to date. This limited collection is represented by only two thermophiles (T. phaeum and Thermotoga lettingae [51]), one that is thermotolerant (Tepidanaerobacter acetoxydans [19]), and two mesophiles (Clostridium ultunense [17] and Syntrophaceticus schinkii [52]). Recent studies have emphasized that the appearance of SAOB in anaerobic systems is more widespread than those characterized by culturedependent methods, and several genomes representing uncultured putative SAOB have been deduced (21,22,41).…”

Section: Discussionmentioning

confidence: 99%

Quantitative Metaproteomics Highlight the Metabolic Contributions of Uncultured Phylotypes in a Thermophilic Anaerobic Digester

Hagen

Frank

Zamanzadeh

et al. 2017

Appl Environ Microbiol

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In this study, we used multiple meta-omic approaches to characterize the microbial community and the active metabolic pathways of a stable industrial biogas reactor with food waste as the dominant feedstock, operating at thermophilic temperatures (60°C) and elevated levels of free ammonia (367 mg/liter NH 3 -N). The microbial community was strongly dominated (76% of all 16S rRNA amplicon sequences) by populations closely related to the proteolytic bacterium Coprothermobacter proteolyticus. Multiple Coprothermobacter-affiliated strains were detected, introducing an additional level of complexity seldom explored in biogas studies. Genome reconstructions provided metabolic insight into the microbes that performed biomass deconstruction and fermentation, including the deeply branching phyla Dictyoglomi and Planctomycetes and the candidate phylum "Atribacteria." These biomass degraders were complemented by a synergistic network of microorganisms that convert key fermentation intermediates (fatty acids) via syntrophic interactions with hydrogenotrophic methanogens to ultimately produce methane. Interpretation of the proteomics data also suggested activity of a Methanosaeta phylotype acclimatized to high ammonia levels. In particular, we report multiple novel phylotypes proposed as syntrophic acetate oxidizers, which also exert expression of enzymes needed for both the Wood-Ljungdahl pathway and ␤-oxidation of fatty acids to acetyl coenzyme A. Such an arrangement differs from known syntrophic oxidizing bacteria and presents an interesting hypothesis for future studies. Collectively, these findings provide increased insight into active metabolic roles of uncultured phylotypes and presents new synergistic relationships, both of which may contribute to the stability of the biogas reactor.IMPORTANCE Biogas production through anaerobic digestion of organic waste provides an attractive source of renewable energy and a sustainable waste management strategy. A comprehensive understanding of the microbial community that drives anaerobic digesters is essential to ensure stable and efficient energy production. Here, we characterize the intricate microbial networks and metabolic pathways in a thermophilic biogas reactor. We discuss the impact of frequently encountered microbial populations as well as the metabolism of newly discovered novel phylotypes that seem to play distinct roles within key microbial stages of anaerobic digestion in this stable high-temperature system. In particular, we draft a metabolic scenario whereby multiple uncultured syntrophic acetate-oxidizing bacteria are capable of syntrophically oxidizing acetate as well as longer-chain fatty acids (via the ␤-oxidation and Wood-Ljundahl pathways) to hydrogen and carbon dioxide, which methanogens subsequently convert to methane.

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Section: Discussionmentioning

confidence: 99%

Quantitative Metaproteomics Highlight the Metabolic Contributions of Uncultured Phylotypes in a Thermophilic Anaerobic Digester

Hagen

Frank

Zamanzadeh

et al. 2017

Appl Environ Microbiol

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“…For C. ultunense, acetate oxidation using Fe(III) as an electron acceptor has been excluded [22,23]. In P. lettingae, acetate oxidation has been observed in the presence of thiosulphate [25]. In order to provide reduced ferredoxin for anabolic and catabolic pathways, both Rnf complex and Ech hydrogenases can reverse the electron flow using the electrochemical gradient and drive the thermodynamically unfavourable reduction of ferredoxin with NADH or hydrogen.…”

Section: Energy Conservation Associated With Saomentioning

confidence: 99%

“…However, our study revealed that at least two pathways for mesophilic acetate oxidation must exist, as the genome of C. ultunense lacks key enzymes of the WL pathway (Figure 4, 5). In the case of the thermophilic P. lettingae, the lack of WL pathway genes was expected, as the metabolism has been described as non-acetogenic [25] (Figure 4 All SAOB are potentially able to activate acetate to acetyl phosphate via acetate kinase ( Figure 4). In the case of S. schinkii and Th.…”

Section: Preprints (Wwwpreprintsorg) | Not Peer-reviewed | Posted: mentioning

confidence: 99%

“…Clostridium ultunense was shown to be able to form endospores, a trait common to all SAOB except P. lettingae [22][23][24][25][26]. The master regulator Spo0A needed for sporulation [63] is encoded (CUESP_1786; AZSU01_30330), but the C. ultunense genome lacks genes encoding the phosphorylases (Spo0F, Spo0B), as has been observed in other clostridia [63].…”

Section: Morphological and Physiological Traitsmentioning

confidence: 99%

“…Whole cell extract-based enzyme activities and mRNA expression studies indicate that the SAOB use a reversal of the WL pathway for oxidising acetate to hydrogen and carbon dioxide [14,27,29,30]. The thermophilic P. lettingae does not express acetogenic metabolism [25] and must therefore use an alternative pathway. T. acetatoxydans might also use another metabolic route for acetate oxidation, as genome-scale analysis has revealed a lack of F0/F1-ATP synthase, which makes use of the oxidative WL pathway for acetate oxidation energetically unfeasible [31].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Guided Analysis of the Syntrophic Acetate Oxidizer <em>C. ultunense</em> and Comparative Genomics Reveal Different Strategies for Acetate Oxidation and Energy Conservation in Syntrophic Acetate-Oxidising Bacteria

Manzoor¹,

Schnürer²,

Bongcam‐Rudloff³

et al. 2018

Preprint

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ABSTRACTschinkii, were identified in C. ultunense and P. lettingae. Moreover, two respiratory complexes sharing similarities to the proton-translocating ferredoxin:NAD + oxidoreductase (Rnf) and the Na+ pumping NADH:quinone hydrogenase (NQR) were predicted. These might form a respiratory chain involved in reduction of electron acceptors other than protons.However, involvement of these complexes in acetate oxidation in C. ultunense and P.lettingae needs further study. This genome-based comparison provides a solid platform for future meta-proteomics and meta-transcriptomics studies and for metabolic engineering, control and monitoring of SAOB.

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Physiological, metabolic and biotechnological features of extremely thermophilic microorganisms

Counts

Zeldes

Lee

et al. 2017

WIREs Mechanisms of Disease

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The current upper thermal limit for life as we know it is approximately 120°C. Microorganisms that grow optimally at temperatures of 75°C and above are usually referred to as ‘extreme thermophiles’ and include both bacteria and archaea. For over a century, there has been great scientific curiosity in the basic tenets that support life in thermal biotopes on earth and potentially on other solar bodies. Extreme thermophiles can be aerobes, anaerobes, autotrophs, heterotrophs, or chemolithotrophs, and are found in diverse environments including shallow marine fissures, deep sea hydrothermal vents, terrestrial hot springs – basically, anywhere there is hot water. Initial efforts to study extreme thermophiles faced challenges with their isolation from difficult to access locales, problems with their cultivation in laboratories, and lack of molecular tools. Fortunately, because of their relatively small genomes, many extreme thermophiles were among the first organisms to be sequenced, thereby opening up the application of systems biology-based methods to probe their unique physiological, metabolic and biotechnological features. The bacterial genera Caldicellulosiruptor, Thermotoga and Thermus, and the archaea belonging to the orders Thermococcales and Sulfolobales, are among the most studied extreme thermophiles to date. The recent emergence of genetic tools for many of these organisms provides the opportunity to move beyond basic discovery and manipulation to biotechnologically relevant applications of metabolic engineering.

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Thermotoga lettingae sp. nov., a novel thermophilic, methanol-degrading bacterium isolated from a thermophilic anaerobic reactor.

Abstract: Thermotoga lettingae sp. nov., a novel thermophilic, methanol-degrading bacterium isolated from a thermophilic anaerobic reactor

Cited by 75 publications

References 45 publications

Quantitative Metaproteomics Highlight the Metabolic Contributions of Uncultured Phylotypes in a Thermophilic Anaerobic Digester

Quantitative Metaproteomics Highlight the Metabolic Contributions of Uncultured Phylotypes in a Thermophilic Anaerobic Digester

Genome-Guided Analysis of the Syntrophic Acetate Oxidizer <em>C. ultunense</em> and Comparative Genomics Reveal Different Strategies for Acetate Oxidation and Energy Conservation in Syntrophic Acetate-Oxidising Bacteria

Physiological, metabolic and biotechnological features of extremely thermophilic microorganisms

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