Thermosynechococcaceae as a source of thermostable C-phycocyanins: properties and molecular insights

Liang, Yuanmei; Kaczmarek, Michal B.; Kasprzak, Agnieszka; Tang, Jie; Shah, Md. Mahfuzur Rahman; Jin, Peng; Klepacz-Smółka, Anna; Ji, Cheng; Ledakowicz, S.; Daroch, Maurycy

doi:10.1016/j.algal.2018.08.037

Cited by 50 publications

(37 citation statements)

References 48 publications

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“…Further structural comparisons of the ( β ) 6 PC hexamers from cyanobacterial species from different habitats revealed three symmetrical "hot spots" that potentially contribute to thermostability (Fig. 3A, 3B) (Liang et al, 2018). The hot spots included five residue substitutions located on -helix B of the -subunit at positions 21, 28, 33, 37 and 42, and one at position 145 (Fig.…”

Section: Structural Characteristics Of Thermostable Phycocyaninmentioning

confidence: 96%

“…in terms of absorbance, indicative of protein denaturation), with 22% and 48% losses following incubation at approximately 50 °C and 60 °C, respectively, for 30 minutes at pH 7 (Patel et al, 2004;Chaiklahan et al, 2012;Wu et al, 2016). In contrast, PC extracted from S. lividus PCC 6715 showed only a 15% loss at 60 °C for 5 hours at pH 7, and no losses at 50 °C for 4 hours within a pH range of 4 to 8 (Liang et al, 2018). Measurements based on fluorescence of PC extracted from T. elongatus TA-1 also showed minimal losses of PC (i.e.…”

Section: Phycobiliproteins In Thermophilesmentioning

confidence: 99%

“…The structure and composition of PC has been extensively studied, and several studies have examined how specific residues contribute to increased structural rigidity and thermostability in thermophilic species (Adir et at al., 2001;Pittera et al, 2017;Liang et al, 2018). Notably, the degree of homology between PC -and β -subunits from different species is high, while crystal structures from a variety of species have shown a great deal of similarity (Adir et at al., 2001).…”

Section: Structural Characteristics Of Thermostable Phycocyaninmentioning

confidence: 99%

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Phycobiliproteins from extreme environments and their potential applications

Puzorjov

McCormick²

2020

Journal of Experimental Botany

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The light-harvesting phycobilisome complex is an important component of photosynthesis in cyanobacteria and red algae. Phycobilisomes are composed of phycobiliproteins, including the blue phycobiliprotein phycocyanin, that are considered high-value products with applications in several industries. Remarkably, several cyanobacteria and red algal species retain the capacity to harvest light and photosynthesise under highly selective environments such as hot springs, and flourish in extremes of pH and elevated temperatures. These thermophilic organisms produce thermostable phycobiliproteins, which have superior qualities much needed for wider adoption of these natural pigment–proteins in the food, textile, and other industries. Here we review the available literature on the thermostability of phycobilisome components from thermophilic species and discuss how a better appreciation of phycobiliproteins from extreme environments will benefit our fundamental understanding of photosynthetic adaptation and could provide a sustainable resource for several industrial processes.

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Section: Structural Characteristics Of Thermostable Phycocyaninmentioning

confidence: 96%

Section: Phycobiliproteins In Thermophilesmentioning

confidence: 99%

Section: Structural Characteristics Of Thermostable Phycocyaninmentioning

confidence: 99%

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Phycobiliproteins from extreme environments and their potential applications

Puzorjov

McCormick²

2020

Journal of Experimental Botany

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“…Additionally, PC from Thermosynechococcus elongatus TA-1 was stable between 4 and 60 • C at a pH range of 4 to 9, but PC denaturation occurred at temperatures over 75 • C [44]. Likewise, the thermostable PC from the Synechococcus lividus PCC 6715 strain that was isolated from a hot spring maintained 90% and 70% stability at 50 • C for 5 h and two weeks, respectively [45]. Additionally, PC from the thermophilic red algae Cyanidioschyzon merolae showed a midpoint denaturation at 83 • C and pH 5, with a half-life of 40 min [46].…”

Section: Pbps Stability Under Different Temperature Regimesmentioning

confidence: 97%

Use of Phycobiliproteins from Atacama Cyanobacteria as Food Colorants in a Dairy Beverage Prototype

Galetovic

Seura

Gallardo

et al. 2020

Foods

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The interest of the food industry in replacing artificial dyes with natural pigments has grown recently. Cyanobacterial phycobiliproteins (PBPs), phycoerythrin (PE) and phycocyanin (PC), are colored water-soluble proteins that are used as natural pigments. Additionally, red PE and blue PC have antioxidant capabilities. We have formulated a new food prototype based on PBP-fortified skim milk. PBPs from Andean cyanobacteria were purified by ammonium sulfate precipitation, ion-exchange chromatography, and freeze-drying. The stability of PE and PC was evaluated by changes in their absorption spectra at various pH (1–14) and temperature (0–80 °C) values. Purified PBPs showed chemical stability under pH values of 5 to 8 and at temperatures between 0 and 50 °C. The antioxidant property of PBP was confirmed by ABTS (2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical ion scavenging, and FRAP (Ferric Antioxidant Power) assays. The absence of PBP toxicity against Caenorhabditis elegans was confirmed up to 1 mg PBP/mL. Skim milk fortified with PE obtained a higher score after sensory tests. Thus, a functional food based on skim milk-containing cyanobacterial PBPs can be considered an innovative beverage for the food industry. PBPs were stable at an ultra-high temperature (138 °C and 4 s). PBP stability improvements by changes at its primary structure and the incorporation of freeze-dried PBPs into sachets should be considered as alternatives for their future commercialization.

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“…Thermosynechococcus elongatus BP-1 is a thermophilic cyanobacterium with optimal growth at 55 °C, making it ideal for biotechnology applications that require high temperatures [79]. T. elongatus BP-1 proteins are also ideal for purification and crystallographic studies, due to their increased stability at high temperatures [80]. A natural transformation method for chromosomal integration has been developed for T. elongatus BP-1, with either kanamycin or chloramphenicol used for selection of transformants [81].…”

Section: “Non-model” Species: Requirements For Uptake and Genetic mentioning

confidence: 99%

Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology

et al. 2019

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Recent advances in synthetic biology and an emerging algal biotechnology market have spurred a prolific increase in the availability of molecular tools for cyanobacterial research. Nevertheless, work to date has focused primarily on only a small subset of model species, which arguably limits fundamental discovery and applied research towards wider commercialisation. Here, we review the requirements for uptake of new strains, including several recently characterised fast-growing species and promising non-model species. Furthermore, we discuss the potential applications of new techniques available for transformation, genetic engineering and regulation, including an up-to-date appraisal of current Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein (CRISPR/Cas) and CRISPR interference (CRISPRi) research in cyanobacteria. We also provide an overview of several exciting molecular tools that could be ported to cyanobacteria for more advanced metabolic engineering approaches (e.g., genetic circuit design). Lastly, we introduce a forthcoming mutant library for the model species Synechocystis sp. PCC 6803 that promises to provide a further powerful resource for the cyanobacterial research community.

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Thermosynechococcaceae as a source of thermostable C-phycocyanins: properties and molecular insights

Cited by 50 publications

References 48 publications

Phycobiliproteins from extreme environments and their potential applications

Phycobiliproteins from extreme environments and their potential applications

Use of Phycobiliproteins from Atacama Cyanobacteria as Food Colorants in a Dairy Beverage Prototype

Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology

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