Thermostable vaccines for Newcastle disease: a review

Mahmood, Muhammad Shahid; Siddique, Faisal; Hussaın, Iftikhar; Ahmad, Shamsheer; Rafique, Azhar

doi:10.1017/s0043933914000889

Cited by 19 publications

(5 citation statements)

References 44 publications

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“…However, heat-resistant strains (such as V4) can maintain their hemagglutinin (HA) activity and infectious ability at 56 • C for at least 30 min (Simmons, 1967). Previous studies showed that most thermostable strains belong to genotype I and possess low pathogenicity to chickens, and the thermostable vaccines have been widely used in areas where a "cold-chain" is not reliable (Mahmood et al, 2014;Dimitrov et al, 2017). Previously, the F, HN and P genes have been studied regarding their relations with virus thermostability by constructing chimeric viruses based on V4 (Wen et al, 2016;Zhao et al, 2018;Liu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Residues 315 and 369 in HN Protein Contribute to the Thermostability of Newcastle Disease Virus

Ruan

Zhang

et al. 2020

Front. Microbiol.

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Thermostable Newcastle disease virus (NDV) vaccines have been widely used in areas where a "cold-chain" is not reliable. However, the molecular mechanism of NDV thermostability remains poorly understood. In this work, we constructed chimeric viruses by exchanging viral fusion (F) and/or hemagglutinin-neuraminidase (HN) genes between the heat-resistant strain HR09 and thermolabile strain La Sota utilizing a reverse genetic system. The results showed that only chimeras with HN derived from the thermostable virus exhibited a thermostable phenotype at 56 • C. The hemagglutinin (HA) and neuraminidase (NA) activities of chimeras with HN derived from the HR09 strain were more thermostable than those containing HN from the La Sota strain. Then, we used molecular dynamics simulation at different temperatures (310 K and 330 K) to measure the HN protein of the La Sota strain. The conformation of an amino acid region (residues 315-375) was observed to fluctuate. Sequence alignment of the HN protein revealed that residues 315, 329, and 369 in the La Sota strain and thermostable strains differed. Whether the three amino acid substitutions affected viral thermostability was investigated. Three mutant viruses based on the thermolabile strain were generated by substituting one, two or three amino acids at positions 315, 369, and 329 in the HN protein. In comparison with the parental virus, the mutant viruses containing mutations S315P and I369V possessed higher thermostablity and HA titers, NA and fusion activities. Taken together, these data indicate that the HN gene of NDV is a major determinant of thermostability, and residues 315 and 369 have important effects on viral thermostability.

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Section: Introductionmentioning

confidence: 99%

Residues 315 and 369 in HN Protein Contribute to the Thermostability of Newcastle Disease Virus

Ruan

Zhang

et al. 2020

Front. Microbiol.

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“…The better performance of this vaccine, in part, may be associated with its thermostability. I-2-based NDV vaccines are stable at room temperature for several days [10,20]. Though due care was given to maintain cold chain at our end, it is uncertain whether there was any breakage in the proper cold chain maintenance at any stage of the vaccine storage and transportation chain.…”

Section: Discussionmentioning

confidence: 99%

Safety and Immunogenicity of ND Vaccines Used in Nepal

Marahatta,

Upadhyaya,

Shah

et al. 2023

Nep. Vet. J

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Viral infections, including Newcastle disease (ND), constitute a major health problem in the rapidly growing poultry industry of Nepal. Despite regular vaccination in the commercial farms, ND virus (NDV) outbreaks are frequently reported raising concern over the safety and immunogenicity of live-attenuated vaccines being used. This study was performed to compare the safety and immunogenicity of four commercially available ND vaccines in Nepal after administration at recommended (1X) and higher (10X) dose. There was no safety concern associated with the use of lentogenic live-attenuated ND vaccines even at higher dose. A varying degree of antibody response was observed at recommended and higher doses with the thermostable I-2 vaccine outperforming other groups. A higher dose did not improve antibody response except for the F1 vaccine. To prevent widespread outbreaks in future, regular molecular surveillance to identify the circulating strains of NDV together with the periodic evaluation of immunogenicity and protective efficacy of commercial vaccines is necessary in Nepal.

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“…Moreover, investigations on rural flocks have revealed that I‐2 originated vaccines were associated with approximately 80% coverage and long‐term immunity (Adwar and Lukesova, 2008). Historically, thermostable ND vaccines have been developed at the Queensland University and used in several developing countries to control ND in rural flocks (Meers & Spradbrow, 2006); however, due to more stability in ambient temperature and lateral transmission, the thermostable ND vaccines are usually associated with a lower variation in antibody titres and cause better protection against ND (Alders & Spradbrow, 2001; Illango et al., 2008; Shahid Mahmood et al., 2014). These advantages have made the thermostable ND vaccine as a good option for the use in commercial broiler farms.…”

Section: Discussionmentioning

confidence: 99%

Thermoresistant Newcastle disease vaccine effectively protects SPF, native, and commercial chickens in challenge with virulent virus

Abdoshah

Hassanzadeh

Masoudi

et al. 2022

Veterinary Medicine & Sci

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Background Due to the more stability and a better homogenecity in immune response, the use of thermoresistant vaccines in different chicken types has been increased. Objective This study aimed to evaluate the efficacy of a newly developed Newcastle disease vaccine (ND.TR.IR) originating from I‐2 strain in specific pathogen‐free (SPF) and native and broiler chickens. Methods Following determination of pathogenicity indices on the candidate seed, three efficacy examinations were conducted. In the first experiment, 120 1‐day‐old SPF chickens were randomly allocated to six groups and either vaccinated with ND.TR.IR via eye drop at 1, 7, and 21 days of age (V1, V7, and V21), or considered as non‐vaccinated control groups (C1, C7, and C21). At 20th post‐vaccination day, sera hemagglutination inhibition (HI) antibody titres against ND virus (NDV) were measured and then the chickens were challenged by virulent NDV (vNDV). In the second and third experiments, the efficacy of ND.TR.IR vaccine was compared to routine vaccination program (B1 and LaSota) in native and broiler chickens that were vaccinated at 10 and 20 days of age, respectively. The HI antibody titres were measured on 10, 20, 30, and 40 days of age, and also challenge efficacy test with vNDV was conducted on 30 days of age. Results The studied virus, as a vaccinal seed, complied with the pathogenicity indices of avirulent NDV and molecular identity of I‐2 strain. In the efficacy evaluation trials, the vaccinated chickens had higher HI antibody titres against NDV compared with their corresponding control chickens (p < 0.05). Results of the challenge tests indicated 95% and 100% protection against vNDV in native, SPF, and broiler‐vaccinated chickens, respectively. Conclusions The present findings indicated that administration of ND.TR.IR induced appropriate HI antibody titres against NDV in SPF, native, and broiler chickens associated with good protection in efficacy test.

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Thermostable vaccines for Newcastle disease: a review

Cited by 19 publications

References 44 publications

Residues 315 and 369 in HN Protein Contribute to the Thermostability of Newcastle Disease Virus

Residues 315 and 369 in HN Protein Contribute to the Thermostability of Newcastle Disease Virus

Safety and Immunogenicity of ND Vaccines Used in Nepal

Thermoresistant Newcastle disease vaccine effectively protects SPF, native, and commercial chickens in challenge with virulent virus

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