Thermostable and alkaline-tolerant microbial cellulase-free xylanases produced from agricultural wastes and the properties required for use in pulp bleaching bioprocesses: a review

Techapun, Charin; Poosaran, Naiyatat; Watanabe, Masanori; Sasaki, Kohsuke

doi:10.1016/s0032-9592(02)00331-x

Cited by 166 publications

(96 citation statements)

References 98 publications

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“…Xylanases have att racted considerable att ention not only for their potential application in lignocellulose hydrolysis and their bioconversion into sugars (14), but also for juice clarifi cation, vegetable oil extraction, improvement of animal digestion, fl our for baked goods, and bleaching paper pulp (15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

Carvalho

Neto

Almeida

et al. 2015

Food Technol. Biotechnol.

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Currently, lignocellulosic waste is a topic of global studies, given that fossil fuel reserves are diminishing, the new agricultural frontiers are limited and the demand for food and biofuels is increasing by the growing world population (1). For these reasons, technology must advance to improve the use of agricultural and agroindustrial residues to obtain food and biofuel.Xylans present in lignocellulosic materials have been studied for obtaining xylooligosaccharides (XOS) from waste materials such as corncobs, rice hulls, olive pits, barley straw (2), tobacco stalk, cott on stalk, sunfl ower stalk, wheat straw (3) and sugarcane bagasse (4-6). Sugarcane bagasse is inexpensive, renewable and abundant source of XOS especially in the countries that produce ethanol and sugar from sugarcane. However, more research is necessary to improve the use of this residue. SummarySugarcane bagasse is an important lignocellulosic material studied for the production of xylooligosaccharides (XOS). Some XOS are considered soluble dietary fi bre, with low caloric value and prebiotic eff ect, but they are expensive and not easily available. In a screening of 138 fungi, only nine were shortlisted, and just Aspergillus fumigatus M51 (35.6 U/mL) and A. fumigatus U2370 (28.5 U/mL) were selected as the most signifi cant producers of xylanases. These fungi had low β-xylosidase activity, which is desirable for the production of XOS. The xylanases from Trichoderma reesei CCT 2768, A. fumigatus M51 and A. fumigatus U2370 gave a signifi cantly higher XOS yield, 11.9, 14.7 and 7.9 % respectively, in a 3-hour reaction with hemicellulose from sugarcane bagasse. These enzymes are relatively thermostable at 40-50 °C and can be used in a wide range of pH values. Furthermore, these xylanases produced more prebiotic XOS (xylobiose and xylotriose) when compared with a commercial xylanase. The xylanases from A. fumigatus M51 reached a high level of XOS production (37.6 %) in 48-72 h using hemicellulose extracted from sugarcane bagasse. This yield represents 68.8 kg of prebiotic XOS per metric tonne of cane bagasse. In addition, in a biorefi nery, aft er hemicellulose extraction for XOS production, the residual cellulose could be used for the production of second-generation ethanol.

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Section: Introductionmentioning

confidence: 99%

Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

Carvalho

Neto

Almeida

et al. 2015

Food Technol. Biotechnol.

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“…Âîíè ï³äâèùóþòü ðîçùåïëåííÿ îñàäжåíîгî êñèëàíó, ÿêèé óòâîðèâñÿ íà ïîâåðõí³ öåëþëîçíèõ âîëîêîí ï³ñëÿ îáðîáêè. цå âèêëèêàº ï³äâèùåííÿ ïðîíèêíîñò³ âîëîêîí äåðåâèíè äî â³äá³ëþþ÷èõ õ³ì³êàò³â ³ äîçâîëÿº ïðîõîäжåííÿ âåëèêèõ фðàгìåíò³â ë³гí³íó ç äåðåâèíè [4,52]. Êñèëàíàçè ì³êðîîðгàí³çì³â, êð³ì öåëþëîçíî ïàðåðîâî¿ ïðîìèñëîâîñò³, çíàõîäÿòü çàñòîñóâàííÿ â õàð÷îâ³é.…”

Section: практичне застосуванняunclassified

Xylanases of Microorganisms

Varbanets¹,

Borzova²

2007

Microbiology&Biotechnology

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ÊCèËàÍàçè ÌiÊÐÎÎÐÃàÍiçÌiâÍàÿâí³ñòü âåëèêèõ ðåñóðñ³â öåëþëîçè ³ гåì³öåëþëîçè â ðîñëèíí³é á³îìàñ³ ðîáèòü âñå á³ëüø àêòóàëüíîþ ïðîáëåìó çàëó÷åííÿ ðîñëèííèõ â³äõîä³â ñ³ëüñüêîгî гîñïîäàðñòâà ³ ïðîìèñëîâîñò³ â ïðîöåñè ì³êðîáíî¿ êîíâåðñ³¿ äëÿ îòðèìàííÿ õàð÷îâîгî ³ êîðìîâîгî á³ëêà ³ ф³ç³îëîг³÷íî àêòèâíèõ ðå÷îâèí. Â³ä 20 äî 40 % â³ä ñóõî¿ âàгè ñ³ëüñüêîгîñïîäàðñüêèõ â³äõîä³â ñêëàäàº êñèëàí, ÿêèé ðàçîì ç гåì³öåëþëîçîþ óòâîðþº äðóгèé íàéá³ëüø øèðîêî ðîçïîâñþäжåíèé â á³îñфåð³ ïîë³ñàõàðèä, çäàòíèé äî â³äíîâëåííÿ. Áóëî îö³íåíî, ùî 500 ìëí òîí òàêèõ ìàòåð³àë³â ìîжóòü áóòè ùîð³÷íî äîñòóïí³ ò³ëüêè â³ä çàëèøê³â гîëîâíèõ çåðíîâèõ êóëüòóð. Òîìó ðîçâèòîê ìàëîêîøòîâíèõ òåõíîëîг³é, ÿê³ îñíîâàí³ íà гåì³öåëþëîç³, âèêëèêàº ³íòåðåñ ó äîñë³äíèê³â. йìîâ³ðíî, â íåäàëåêîìó ìàéáóòíüîìó, êñèëàí â êîìá³íàö³¿ ç öåëþëîçîþ çàáåçïå÷èòü á³ëüø³ñòü ñâ³òîâèõ ïîòðåá â ñèðîâèí³. Ìîжëèâî ïåðåäáà÷èòè, ùî â íàñòóïí³ 50 ðîê³â âóг³ëëÿ òà íåî÷èùåíà íàфòà áóäóòü çàì³íåí³ á³îìàñîþ. Â áàгàòüîõ òåõíîëîг³÷íèõ ïðîöåñàõ, ïåðø çà âñå ïðè âèðîáíèöòâ³ ïàïåðó òà öåëþëîçè, êñèëàí º íåáàжàíèì äîì³øêîì, ³ ïîâñòàº ïðîáëåìà éîгî äåгðàäàö³¿. Нåîáõ³äíî г³äðîë³çóâàòè êñèëàí òàêîж â äåÿêèõ ïðîöåñàõ õàð÷îâî¿ ³ êîðìîâî¿ ïðîìèñëîâîñò³, ïðè êîìïëåêñí³é ïåðåðîáö³ ðîñëèííèõ â³äõîä³â. Ã³äðîë³ç êñèëàíó ìîжå áóòè çä³éñíåíèé çà äîïîìîгîþ õ³ì³÷íèõ ðåàгåíò³â àáî фåðìåíòàòèâíèì øëÿõîì. Ôåðìåíòàòèâíèé г³äðîë³ç º ïåðåâàжëèâèì, îñê³ëüêè ìåíø âïëèâàº íà ñòðóêòóðó ³íøèõ âîëîêîí ³ óòâîðþºòüñÿ ìåíøå õ³ì³÷íèõ â³äõîä³â. Â õàð÷îâ³é àáî êîðìîâ³é ïðîìèñëîâîñòÿõ õ³ì³÷íèé г³äðîë³ç âçàгàë³ íåïðèéíÿòíèé. 9 Ìікробіоëоãія і біотеõноëоãія ¹ 3/2008ÊCиËÀНÀÇи ÌIÊÐÎÎÐÃÀНIÇÌIÂ Äëÿ ì³êðîáíîгî г³äðîë³çó êñèëàíó íåîáõ³äíà ä³ÿ òàêèõ фåðìåíò³â, ÿê β 1,4 êñèëàíàçè ³ β êñèëîçèäàçè. еíäî 1,4 β d êñèëàíàçà àáî 1,4 β d êñèëàí êñèëàíîг³äðîëàçà (ÊÔ 3.2.1.8) -фåðìåíò, ÿêèé çä³éñíþº íåóïîðÿäêîâàíèé г³äðîë³ç âíóòð³øíüîìîëåêóëÿðíèõ êñèëîçèäíèõ çâ'ÿçê³â êñèëàí³â àáî êñèëîîë³гîñàõàðèä³â. Âèñîêîìîëåêóëÿðí³ êñèëàíè ìàþòü ïåðåâàгó äëÿ ä³¿ фåðìåíòó â ïîð³âíÿíí³ ç îë³гîìåðíèìè ëàíöþгàìè, øâèäê³ñòü г³äðîë³çó ÿêèõ çíèжóºòüñÿ ç³ çìåíøåííÿì ñòóïåíÿ ïîë³ìåð³çàö³¿. Äðóгèé фåðìåíò, ÿêèé áåðå ó÷àñòü â ðîçùåïëåíí³ êñèëàí³â, -åêçî 1,4 β d êñèëîçèäàçà (ÊÔ 3.2.1.37) àáî β êñèëîçèäàçà, ÿêà êàòàë³çóº г³äðîë³ç îë³гîñàõàðèä³â øëÿõîì ïîñë³äîâíîгî â³äùåïëåííÿ çàëèøê³â d êñèëîçè ç íåðåäóêóþ÷îгî ê³íöÿ. Âñòàíîâëåíî, ùî àêòèâí³ñòü êñèëîçèäàçè íàéá³ëüøà ïî â³äíîøåííþ äî êñèëîá³îçè ³ çìåíøóºòüñÿ ç³ çá³ëüøåííÿì äîâжèíè ëàíöþгà ñóáñòðàòà.Äжåðåëàìè êñèëàíàç ìîжóòü áóòè áåçõðåáåòí³ òâàðèíè, âèù³ ðîñëèíè, íàñ³ííÿ, ìîðñüê³ âîäîðîñò³, àëå íàéá³ëüø òåõíîëîг³÷íèìè º ì³êðîîðгàí³çìè -гðèáè, áàêòåð³¿, äð³жäж³ [1]. Ì³êðîáí³ êñèëàíàçè çàâäÿêè ¿õ âèñîê³é ñïåöèф³÷íîñò³, ì'ÿêèì óìîâàì ðåàêö³¿, íåçíà÷íèì âèòðàòàì ñóáñòðàòà ³ óòâîðåííþ ïîá³÷íèõ ïðîäóêò³â, º íàéá³ëüø ïåðñïåêòèâíèìè êàòàë³çàòîðàìè äëÿ г³äðîë³çó êñèëàíó. Àëå âàðò³ñòü фåðìåíòàòèâíîгî г³äðîë³çó á³îìàñè º îäíèì ç фàêòîð³â, ÿêèé ë³ì³òóº åêîíîì³÷íó äîö³ëüí³ñòü ïðîöåñó. Ïðîäóêö³ÿ êñèëàíàç òèì íå ìåíø ìîжå áóòè ïîêðàùåíà çíàõîäжåííÿì á³ëüø ïîòóжíèõ øòàì³â гðèá³â àáî áàêòåð³é, àáî îäåðжàííÿì ìóòàíòíèõ øòàì³â, çäàòíèõ ïðîäóêóâà...

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“…This facilitates pulp bleaching and lowers chlorine consumption thereby reducing the discharge of toxic-organochlorine compounds in the environment (Senior et al 1992, Tolan andCanovas 1992). Pre-bleaching of paper pulp also requires the * Corresponding author Email: subeddev@yahoo.com use of cellulase free xylanases, since cellulases may adversely affect the quality of the paper pulp by destroying the structure of cellulose and thus diminish the quality of the pulp (Techapun et al 2003). For the development of suitable xylanase as a pre-bleaching agent, the stability of enzyme at higher optimum pH and temperature is desirable (Beg et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Optimization of alkali-thermostable and cellulase-free eylanase production from Bacillus Sp.

et al. 2012

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Context: To analyze the nutritional and physicochemical parameters for the production of alkali-thermostable and cellulase free xylanase from bacteria. Objectives:The aim of this study was to isolation and identification and of alkali-thermostable and cellulase free xylanase producing bacteria from soil as well as optimization of process parameters for xylanase production. Materials and Methods:The bacterium Bacillus sp. was isolated from soil by serial dilution technique on xylan agar medium and identified by morphological and biochemical studies. The production of xylanase was carried out on xylan broth medium and xylanase activity was assayed by dinitrosalicylic acid (DNS) method. The effect of cultural parameters on the production of xylanase was determined by measuring the activity of xylanase. The effect of temperature and pH on the activity of partially purified xylanase as well as substrate specificity of xylanase were examined. Results:The maximum xylanase production (4000 U/L) by a Bacillus sp. was attained when the medium containing 0.5% wheat bran xylan and peptone at pH 8.0 and 50-55°C within 48-60 h. The partially purified xylanase was optimally active at pH 9.0 and 55°C. The xylanase showed high substrate activity towards wheat bran xylan but no activity towards cellulose, carboxymethyl cellulose and starch. Thus the enzyme was alkali-thermostable and cellulase free xylanase. Conclusion:The results obtained in this study suggest that the Bacillus sp. used is highly potential and useful for the production of cellulase free xylanase.

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Thermostable and alkaline-tolerant microbial cellulase-free xylanases produced from agricultural wastes and the properties required for use in pulp bleaching bioprocesses: a review

Cited by 166 publications

References 98 publications

Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

Xylanases of Microorganisms

Optimization of alkali-thermostable and cellulase-free eylanase production from Bacillus Sp.

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