Thermostable Alkaline Serine Protease Production by the Soil Myxobacterium of <i>Archangium</i> sp. UTMC 4504

Rouhizohrab, Nasim; Mohammadipanah, Fatemeh

doi:10.1089/ind.2020.0036

Cited by 3 publications

(1 citation statement)

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“…The culture solid media were incubated under humid conditions (40–60%) at 28°C for up to three weeks. The myxobacterial cells were identified by the detection of fruiting bodies or swarm on agar under the stereomicroscope (Motic, China) ( 15 ).…”

Section: Methodsmentioning

confidence: 99%

Suppression of predominant interfering bacteria in the purification process of myxobacteria

Rouhizohrab¹,

Mohammadipanah²

2022

IJM

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Background and Objectives: Myxobacteria initially recognized by their complex life cycle and social behavior are progres- sively explored for their bioactive secondary metabolites. However, isolation of myxobacteria usually is accompanied by bacterial and fungal contaminations due to the direct cultivation of soilon isolation media, which results in severe challenges in the purification of myxobacteria. Therefore, it is necessary to improve their purification techniquesfrom natural samples to the discovery of new biomolecules. Materials and Methods: In the present study, six physichochemical methods were assessed for their efficacy in the purifi- cation of myxobacterial strains and specially from contaminants of Microvirga spp. Results: Among the evaluated treatments, purification of fruiting bodies using a combination of ultrasonication and heat treatment was identified as theeffective protocol with 80% success rate in the purification of myxobacterial strains and re- ducing up to 90% of the contaminating bacteria. Conclusion: Concerning the problematic contamination of myxobacterial isolates, the introduced approach can retrieve the myxobacterial strains which areoften suppressed by the over growth of contaminations especially root symbiotic bacteria namely Microvirga spp.

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Section: Methodsmentioning

confidence: 99%