Thermostability of photosystem I trimers and monomers from the cyanobacterium Thermosynechococcus elongatus

Shubin, Vladimir; Терехова, И. В.; Bolychevtseva, Yu. V.; El-Mohsnawy, Eithar; Rögner, Matthias; Mäntele, Werner; Kopczak, Marta J.; Džafić, Enela

doi:10.1016/j.saa.2017.02.010

Cited by 8 publications

(3 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Purified PS1 showed an oxygen consumption activity of about 1100 µmol O 2 h −1 mg Chl −1 . The inactivated photosystem (iPS1) was prepared by thermal treatment of PS1 50 using a silicone oil bath at 120 °C for 60 min. Chl a was obtained from the purified PS1 protein sample by incubation for 24 h at room temperature (RT) in absolute EtOH (ThermoFisher Scientific), followed by centrifugation (15,500 × g , RT, 10 min).…”

Section: Methodsmentioning

confidence: 99%

Light-induced formation of partially reduced oxygen species limits the lifetime of photosystem 1-based biocathodes

et al. 2018

Self Cite

View full text Add to dashboard Cite

Interfacing photosynthetic proteins specifically photosystem 1 (PS1) with electrodes enables light-induced charge separation processes for powering semiartificial photobiodevices with, however, limited long-term stability. Here, we present the in-depth evaluation of a PS1/Os-complex-modified redox polymer-based biocathode by means of scanning photoelectrochemical microscopy. Focalized local illumination of the bioelectrode and concomitant collection of H2O2 at the closely positioned microelectrode provide evidence for the formation of partially reduced oxygen species under light conditions. Long-term evaluation of the photocathode at different O2 concentrations as well as after incorporating catalase and superoxide dismutase reveals the particularly challenging issue of avoiding the generation of reactive species. Moreover, the evaluation of films prepared with inactivated PS1 and free chlorophyll points out additional possible pathways for the generation of oxygen radicals. To avoid degradation of PS1 during illumination and hence to enhance the long-term stability, the operation of biophotocathodes under anaerobic conditions is indispensable.

show abstract

Section: Methodsmentioning

confidence: 99%

Light-induced formation of partially reduced oxygen species limits the lifetime of photosystem 1-based biocathodes

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…These results indicated that the pH-responsive precipitation of CspB-Teri was related to the reversible secondary structure change observed by FTIR at 1624 and 1619 cm −1 . Note that the peaks in the FTIR spectra at approximately 1624 and 1620 cm −1 indicate an intermolecular β-sheet structure [ 26 , [30] , [31] , [32] , [33] ]. Thus, the conformation of CspB-Teri was changed to a β-sheet-rich structure as the pH decreased, followed by the formation of intermolecular interactions and precipitation.…”

Section: Resultsmentioning

confidence: 99%

The pH-responsive precipitation–redissolution of the CspB fusion protein, CspB50TEV-Teriparatide, triggered by changes in secondary structure

Nagano¹,

Mannen²,

Kikuchi³

et al. 2023

Biochemistry and Biophysics Reports

View full text Add to dashboard Cite

“…responses are always accompanied by PSI oligomerization, where the trimeric PSI complex is more tolerant than monomeric complex against high temperature, where the α-helices in monomers are more susceptible to temperature elevation than the respective α-helices in trimers. For this reason, oligomerization might be an effective strategy in thermophilic cyanobacterium Thermosynechococcus elongatus to enhance thermostability of PSI complexes (Shubin et al, 2017). Environmental factors can also influence the oligmerization of PSI complex, where under iron deficiency, trimeric PSI complex is splitting into monomeric complex (Ivanov et al, 2006 andEl-Mohsnawy, 2014).…”

Section: Physiologicalmentioning

confidence: 99%

Stabilization of trimeric PSI within Thermosynechococcus elongatus via Chlorophyll a and carotenoids located in monomer-monomer interface area

El-Mohsnawy

2019

Egypt. J. Bot.

Self Cite

View full text Add to dashboard Cite

M ONOmErIC PSI is found within thermophilic cyanobacteria, i.e., Thermosynechococcus elongatus either in case of iron deficiency or deletion of PsaL subunit. Previous investigations pointed to the role of PsaL in oligomerization of monomeric complexes. Successful isolation of monomeric PSI was achieved only via salt treatment of thylakoid membrane at high temperature (50°C). Here, monomeric PSI was obtained through splitting the purified trimeric PSI in vitro. Selective elimination of carotenoids and Chlorophyll a located in monomer-monemer interface area was performed using a mixture of hexane with different organic solvents. Hexane-dimethyl sulfoxide mixture was considered the best organic solvent for removal the interface carotenoids and chlorophyll a, where it splatted trimeric PSI completely. Also, the role of carotenoids and chlorophyll a on stabilization of trimeric PSI was proven by sucrose density gradient and blue native gel. 77K spectroscopical emission fluorescence measurements of investigated PSI showed blue shift peaks due to monemerization as well as formation of uncoupled chlorophylls. The efficiency of organic solvents on removing carotenoids molecules was higher than chlorophyll molecules, where about 12/96 chlorophyll molecules and 12/22 carotenoids molecules have been eliminated in case of complete splitting of trimeric PSI.

show abstract

Thermostability of photosystem I trimers and monomers from the cyanobacterium Thermosynechococcus elongatus

Cited by 8 publications

References 35 publications

Light-induced formation of partially reduced oxygen species limits the lifetime of photosystem 1-based biocathodes

Light-induced formation of partially reduced oxygen species limits the lifetime of photosystem 1-based biocathodes

The pH-responsive precipitation–redissolution of the CspB fusion protein, CspB50TEV-Teriparatide, triggered by changes in secondary structure

Stabilization of trimeric PSI within Thermosynechococcus elongatus via Chlorophyll a and carotenoids located in monomer-monomer interface area

Contact Info

Product

Resources

About