Thermosensitive nanoparticle of mPEG-PTMC for oligopeptide delivery into osteoclast precursors

Abstract

Transmembrane delivery of biomolecules through nanoparticles plays an important role in targeted therapy. Here, we designed a simple nanoparticle for the delivery of model peptide drug into primary osteoclast precursor cells (bone marrow macrophages) by thermosensitive and biodegradable diblock copolymer monomethoxy poly(ethylene glycol)-block-poly(trimethylene carbonate). The model peptide drug was encapsulated into the nanoparticle by dropping the drug carrier dissolved in dimethylsulfoxide solvent into water containing poly(vinyl alcohol) to achieve temperature response nanoparticles. Through size analysis, we found that the nanoparticles possessed a temperature-sensitive property between 30°C and 40°C. Moreover, flow cytometry and spectrofluorimetry analysis indicated that nanoparticle systems underwent significant cellular uptake. In addition, the evaluation of cell biology showed that nanoparticles have excellent biocompatibility. Thus, the results indicated that the temperature-sensitive nanoparticles have potential application value for targeted delivery of oligopeptide in the treatment process of osteoarthritis.

Construction and Mechanism of IL‐15‐Based Coactivated Polymeric Micelles for NK Cell Immunotherapy

“…[20][21][22] The amphiphilic block copolymer PEG-PTMC can self-assemble into micelles through hydrophilic and hydrophobic interactions to prolong the circulation time of loaded drugs in vivo. [23] More importantly, it can also be modified to simultaneously chemically graft multiple drugs in hydrophilic or hydrophobic segments and has proved to be a good carrier for drug delivery.…”