Thermosensitive molecularly imprinted hydrogel cross‐linked with N‐malely chitosan for the recognition and separation of BSA

Abstract

A novel temperature-sensitive molecularly imprinted hydrogel composed of N-isopropylacrylamide and acrylamide has been prepared by using free-radical polymerization and was cross-linked by modified water-soluble N-maley chitosan in aqueous solution. BSA (pI 4.9, MW 66.0 kDa) was used as the template protein. The produced hydrogels were characterized by environmental SEM to reveal the microcosmic morphology. A microporous structure was only found in the imprinted hydrogel, while no obvious microporous structure was found in nonimprinted hydrogels. The lower critical solution temperature of the hydrogels was 34°C, and the optimal binding conditions were tested, namely, the adsorption equilibrium time of 6 h and initial BSA concentration of 1.0 mg/mL. The adsorption capacity Qmax was determined by Langmuir isotherm plots and was 5.72 mg/g for imprinted hydrogel and 1.18 mg/g for nonimprinted hydrogels. A separation factor (β) of 4 was obtained when bovine hemoglobin (pI 6.9, MW 64.0 kDa) was selected as the particular reference protein. Molecular weights and pIs were chosen to investigate the selectivity of the hydrogels. It was shown that the shape memory and the size effect were the major factors for the recognition. This imprinted hydrogel was used to specifically adsorb the BSA from the protein mixture.

Molecular imprinting: perspectives and applications

“…Lightly cross-linked polymer gels can undergo reversible swelling and shrinking in response to environmental temperature changes, called thermo-responsive gels, which can be used as smart materials for drug delivery, tissue engineering, and cell encapsulation in biochemistry. [251][252][253][254][255][256] This type of thermo-sensitive recognition is very similar to the recognition of proteins in natural systems. Thus, thermo-responsive gels with biocompatibility could have potential applications in the design of protein-imprinted polymer matrices.…”

Magnetic-graphene based molecularly imprinted polymer nanocomposite for the recognition of bovine hemoglobin

“…It can be seen that the Q max and K L of MIP were calculated to be 186.73mg/g and 51.30mL/mg, respectively. The correlation coefficients is R 2 =0.998, illustrating that the Langmuir model suits well for BHb adsorption on MIP within the studied concentration range [45]. Besides, the Q max and K L of NIP were calculated to be 95.07mg/g and 22.52mL/mg, which both obviously smaller than those of MIP.…”

Molecularly imprinted electrochemical sensing interface based on in-situ-polymerization of amino-functionalized ionic liquid for specific recognition of bovine serum albumin

“…BSA is very important in maintaining the osmotic homeostasis of blood and transporting small molecules (Hu and Chou, 2009). As one of the most extensively studied proteins, BSA has been widely employed as a template to fabricate molecularly imprinted polymers to investigate their performances in separating and sensing (Kryscio and Peppasa, 2012;Wang and Wei, 2013;Yu et al, 2010;Zhang et al, 2014). Herein, a BSA imprinted polymerized ionic liquid film electrode was facilely fabricated using 3-(3-aminopropyl)-1-vinylimidazole tetrafluoroborate as a functional monomer.…”