Thermosensitive Macroporous Cryogels Functionalized With Bioactive Chitosan/<scp>B</scp>emiparin Nanoparticles

Peniche, Hazel; Reyes-Ortega, Felisa; Aguilar, María Rosa; Rodríguez, Gonzalo Fernández; Abradelo, Cristina; García‐Fernández, Luis; Péniche, Carlos; Román, Julio San

doi:10.1002/mabi.201300184

Cited by 19 publications

(19 citation statements)

References 36 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Many techniques used for the evaluation of thermoresponsive substrates are also capable of determining the chemical structures of thermoresponsive scaffolds, which are frequently characterized using ATR-FTIR, NMR, XPS and SEM/EDS systems [65,[102][103][104][105]. Grafting yield of PNIPAAm mediated scaffolds can be assessed by analyzing the ratio of peak intensities of characteristic and reference peaks from ATR-FTIR spectra.…”

Section: Chemical Featuresmentioning

confidence: 99%

“…Scaffold microstructure (surface and cross-section) is commonly evaluated by SEM, AFM and light microscope like thermoresponsive macrogels [102][103][104][106][107][108]. Pore diameter and distribution or fiber size and alignment are often analyzed by randomly picking at least dozens of various segments by means of image J software [65,109].…”

Section: Morphology and Structurementioning

confidence: 99%

“…The water absorption rate of poriferous scaffolds is determined by soaking them in water or buffer solutions at a required temperature for a predetermined time. The thermoresponsive swelling can be obtained by analyzing the mass difference of dried and hydrated scaffolds [65,68,102,106].…”

Section: Thermoresponsive Swellingmentioning

confidence: 99%

See 2 more Smart Citations

Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

et al. 2020

View full text Add to dashboard Cite

Poly(N-isopropylacrylamide) (PNIPAAm) is a typical thermoresponsive polymer used widely and studied deeply in smart materials, which is attractive and valuable owing to its reversible and remote "on-off" behavior adjusted by temperature variation. PNIPAAm usually exhibits opposite solubility or wettability across lower critical solution temperature (LCST), and it is readily functionalized making it available in extensive applications. Cell culture is one of the most prospective and representative applications. Active attachment and spontaneous detachment of targeted cells are easily tunable by surface wettability changes and volume phase transitions of PNIPAAm modified substrates with respect to ambient temperature. The thermoresponsive culture platforms and matching thermal-liftoff method can effectively substitute for the traditional cell harvesting ways like enzymatic hydrolysis and mechanical scraping, and will improve the stable and high quality of recovered cells. Therefore, the establishment and detection on PNIPAAm based culture systems are of particular importance. This review covers the important developments and recommendations for future work of the preparation and characterization of temperature-responsive substrates based on PNIPAAm and analogues for cell culture applications.

show abstract

Section: Chemical Featuresmentioning

confidence: 99%

Section: Morphology and Structurementioning

confidence: 99%

See 1 more Smart Citation

Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Temperature‐responsive gels with chitosan and PNIPAAm have been studied extensively. Peniche et al . evaluated the biological activity of chitosan and PNIPAAm gels.…”

Section: Introductionmentioning

confidence: 99%

“…8,9 Temperatureresponsive gels with chitosan and PNIPAAm have been studied extensively. Peniche et al 10 chitosan and PNIPAAm gels. In an MTT assay, the chitosan-PNIPAAm gels did not show cytotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Release behavior of a polyanion‐crosslinked chitosan‐poly(N‐isopropylacrylamide) gel thermoresponsive material

Ueoka

Shimomura

Ueda

et al. 2018

J of Applied Polymer Sci

View full text Add to dashboard Cite

A novel type of temperature‐responsive hydrogel with sensitive release performance of an encapsulated compound was obtained. The temperature‐responsive hydrogel with a sensitive state change between 32 °C and 29 °C was prepared by physically crosslinking chitosan with a polyanion of tripolyphosphate and linear poly(N‐isopropylacrylamide) (PNIPAAm). Cyanocobalamin was used as a model drug and was encapsulated in the prepared hydrogel. The swelling ratio and release behavior of cyanocobalamin were evaluated. Hydrogels containing 3.0% chitosan and 3.0% PNIPAAm had the best swelling ratio and the maximum amount of released encapsulated cyanocobalamin. The amount of released cyanocobalamin was quantified at 25 °C and 40 °C to evaluate the release behavior. The encapsulated cyanocobalamin remained within the hydrogels at 25 °C and was rapidly released at 40 °C. After 1 min at 40 °C, the released amount was approximately 50% of the encapsulated cyanocobalamin. The temperature‐responsive hydrogels obtained have good performance for use in drug delivery systems. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46732.

show abstract

Chemie der Chitosan‐Aerogele: Lenkung der dreidimensionalen Poren für maßgeschneiderte Anwendungen

et al. 2020

View full text Add to dashboard Cite

Indiesem Aufsatz diskutieren wir die schnell wachsende Zahl an Literaturbeiträgen zu Chitosan-basierten porçsen Materialien, mit Schwerpunkt auf Gelierungsmechanismen, dreidimensionaler multiskaliger Strukturkontrolle sowie der mannigfaltigen chemischen Funktionalität, die mit anderen Biopolymeren nicht erreichbar ist. Die Eigenschaften unterscheiden sich von letzteren teils stark:v on überkritischg etrockneten, mesoporçsen Chitosan-Aerogelen bis hin zu äußerst leichten, gefriergetrockneten makroporçsen Gerüsten. Im Labormaßstab erreicht porçses Chitosan beeindruckende Eigenschaften, der hoch(meso)porçse Charakter verstärkt jedoch nicht nur die vorteilhafte Funktionalitätdes Chitosans,sondern auchseine Nachteile,was eine mçgliche Industrialisierung ernsthaft einschränkt. Zur Fçrderung des Technologietransfers diskutieren wir in kritischer Weise die praktische Umsetzbarkeit mçglicher Anwendungen von Chitosan-Aerogelen im Vergleich zu konventionellen und anderen biopolymerbasierten porçsen oder nichtporçsen Materialien. Aus dem Inhalt 1. Einleitung 9914 2. Synthese von Chitosan-Aerogelen 9916 3. Prozess-Struktur-Eigenschafts-Beziehungen 9920 4. Oberflächenchemie und Funktionalisierung 9925 5. Anwendungen 9927 6. Zusammenfassung und Ausblick: auf dem Wegz ur Industrialisierung 9932

show abstract

Thermosensitive Macroporous Cryogels Functionalized With Bioactive Chitosan/Bemiparin Nanoparticles

Cited by 19 publications

References 36 publications

Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

Release behavior of a polyanion‐crosslinked chitosan‐poly(N‐isopropylacrylamide) gel thermoresponsive material

Chemie der Chitosan‐Aerogele: Lenkung der dreidimensionalen Poren für maßgeschneiderte Anwendungen

Contact Info

Product

Resources

About