Thermosensitive chitosan-based hydrogel as a topical ocular drug delivery system of latanoprost for glaucoma treatment

Cheng, Yung Hsin; Tsai, Tung Hu; Jhan, Yong Yu; Chiu, Allen W.; Tsai, Kun Ling; Chien, Chian‐Shiu; Chiou, Shih Hwa; Liu, Catherine Jui Lin

doi:10.1016/j.carbpol.2016.02.080

Cited by 103 publications

(53 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Chitosan nanoparticles have biological adhesion properties and may attach to conjunctival cells (9,23,24). As a result, the tight junction between epithelial cells of the cornea and conjunctiva may be transiently opened to increase the permeability of the drug in the cornea and conjunctiva.…”

Section: Discussionmentioning

confidence: 99%

Chitosan temperature‑sensitive gel loaded with drug microspheres has excellent effectiveness, biocompatibility and safety as an ophthalmic drug delivery system

Kong

Zhang

et al. 2017

Exp Ther Med

View full text Add to dashboard Cite

In the present study, a temperature-sensitive gel composed of chitosan, carboxymethyl chitosan and glycerophosphate was prepared and loaded with chitosan microspheres encapsulating levofloxacin. The bioavailability of levofloxacin and the safety of this novel opthalmic drug delivery formulation were evaluated. Levofloxacin chitosan microspheres were prepared using the ionic gelation method, and the particle size and entrapment rate were determined. The morphology of the microspheres was observed by scanning electron microscopy. The pH and zeta potential were measured. The in vitro release of levofloxacin by the chitosan temperature-sensitive gel loaded with drug microspheres was determined using spectrophotometry. The eye retention time of the chitosan temperature-sensitive gel was calculated using a fluorescein sodium test. To assess the bioavailability and safety of the chitosan temperature-sensitive gel, a cell compatibility test, a cytotoxicity test and skin irritation test were performed. The entrapment rate of levofloxacin in the chitosan microspheres was determined to be 26.5%. The levofloxacin chitosan microspheres that were formed by chitosan and sodium tripolyphosphate were identified to be suitable for use in an ophthalmic particle dispersion system based on their physical and chemical properties. The pH of the levofloxacin chitosan microsphere suspension was 5.87±0.04, the average particle diameter was 2,452±342 nm, the polydispersity index was 0.168±0.028 and the ζ potential was 28.62±1.7 mV. The chitosan temperature-sensitive gel carrying microspheres loaded with drug prevented drug burst release at the initial stage and facilitated the slow release of the drug later on. Furthermore, this delivery system markedly prolonged the contact duration of levofloxacin with the eye. The chitosan temperature-sensitive hydrogel was safe and provided a good bioavailability of the drug. The results revealed that the chitosan temperature-sensitive gel had a cytotoxicity of grade 0, and no erythematous response was observed during the entire course of the skin irritation test. The present study provided a basis for the future development of the chitosan-based temperature-sensitive hydrogel in ophthalmic drug delivery.

show abstract

Section: Discussionmentioning

confidence: 99%

Chitosan temperature‑sensitive gel loaded with drug microspheres has excellent effectiveness, biocompatibility and safety as an ophthalmic drug delivery system

Kong

Zhang

et al. 2017

Exp Ther Med

View full text Add to dashboard Cite

show abstract

“…SEM images (Figure ) displayed that the CS/β‐GP/PVP‐I thermosensitive hydrogel presented a highly porous structure that was helpful for iodine releasing from the hydrogel . The iodine released from the hydrogel in the microgap into the oral cavity could perform a local antibacterial function.…”

Section: Discussionmentioning

confidence: 99%

“…(Figure 3) displayed that the CS/β-GP/PVP-I thermosensitive hydrogel presented a highly porous structure that was helpful for iodine releasing from the hydrogel. 51 The iodine released from the hydrogel in the microgap into the oral cavity could perform a local antibacterial function. The antibacterial results ( Figure 8) indicated that all three experimental groups had significant antibacterial effects against E. coli, S. aureus as well as P. gingivalis.…”

Section: Discussionmentioning

confidence: 99%

A thermosensitive chitosan‐based hydrogel for sealing and lubricating purposes in dental implant system

Cao

Cai

Chen

et al. 2019

Clin Implant Dent Rel Res

View full text Add to dashboard Cite

Background Mechanical and biological complications associated with implant systems happen frequently in the clinic. Purpose To develop a chitosan (CS)‐based thermosensitive hydrogel for sealing and lubricating purposes in dental implant system. Materials and Methods In this study, a thermosensitive hydrogel made up of CS, β‐glycerophosphate pentahydrate (β‐GP), and povidone‐iodine (PVP‐I), called CS/β‐GP/PVP‐I thermosensitive hydrogel, was fabricated. Three experimental groups with different volume ratios of CS to β‐GP were prepared, namely 16/4, 13/7, and 10/10 groups. The surface topography of the different groups and their physicochemical characteristics were examined by SEM, FTIR, and X‐ray diffraction analysis. The cytotoxicity of the hydrogel was examined by CCK‐8 test. In vitro antibacterial efficiency was analyzed by the spread plate method. Sealing ability was detected by incubating two‐piece implants in Escherichia coli suspension. Lubricating ability of the hydrogel was evaluated by the removal torque test with a calibrated digital torque meter. Results The CS/β‐GP/PVP‐I thermosensitive hydrogel was fabricated and showed a highly porous structure under SEM. An in vitro cytotoxicity test demonstrated that 13/7 group displayed no cytotoxicity. Furthermore, all three groups showed obviously antibacterial effects. In the sealing ability test, 16/4 group showed the best sealing ability. The removal torque of 16/4 group and 13/7 group was significantly greater than control group. Conclusions Based on our findings, it could be concluded that the thermosensitive and antibacterial CS/β‐GP/PVP‐I hydrogel with sealing and lubricating ability was successfully prepared. The hydrogel had better sealing and lubricating effects when the volume ratio of CS to β‐GP was 16/4.

show abstract

“…These side effects can cause patients to stop using their medications as prescribed, or to not use them at all. This is another reason why novel drug delivery systems such as hydrogels are needed; to reduce the frequency of dosing, reduce side effects and be patient-friendly enough so that patients will use them for an extended period of time if need be (Cheng et al, 2016).…”

Section: Current Commercial Formulations Utilized For the Delivery Ofmentioning

confidence: 99%

“…in terms of the delivery of drugs to the eye as these systems provide an increased retention time at the cornea as well as prevent the rapid removal of the formulation via the nasolacrimal drainage system (Cheng et al, 2016). Both of these factors play a role in overcoming the current challenge of low bioavailability seen in many ocular drug delivery preparations.…”

Section: Stimuli-responsive and In Situ Hydrogel Systems And Their Apmentioning

confidence: 99%

Hydrogel Biomaterials for Application in Ocular Drug Delivery

Lynch

Kondiah

Choonara

et al. 2020

Front. Bioeng. Biotechnol.

145

103

View full text Add to dashboard Cite

Thermosensitive chitosan-based hydrogel as a topical ocular drug delivery system of latanoprost for glaucoma treatment

Cited by 103 publications

References 27 publications

Chitosan temperature‑sensitive gel loaded with drug microspheres has excellent effectiveness, biocompatibility and safety as an ophthalmic drug delivery system

Chitosan temperature‑sensitive gel loaded with drug microspheres has excellent effectiveness, biocompatibility and safety as an ophthalmic drug delivery system

A thermosensitive chitosan‐based hydrogel for sealing and lubricating purposes in dental implant system

Hydrogel Biomaterials for Application in Ocular Drug Delivery

Contact Info

Product

Resources

About