Thermoreversible gelation polymer induces the emergence of hepatic stem cells in the partially injured rat liver

Nagaya, Masaki; Kubota, Sunao; Suzuki, Noboru; Akashi, Katsuya; Mitaka, Toshihiro

doi:10.1002/hep.21153

Cited by 17 publications

(14 citation statements)

References 17 publications

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“…9 days after the spinal cord injury, the spinal cord was reopened at the injury area. were covered with thermo-reversible gelation polymer which had the reversible sol-gel process by temperature 15,16) to retain the injected neurons. The polymer was then covered with a cement protector, and then muscle layers were sutured.…”

Section: )Cell Transplantationmentioning

confidence: 99%

Induction of neural cells with spinal motoneuron phenotype from human iPS cells and the transplantation to totally transected spinal cords in mice

Iinuma

Umehara

Arimitsu

et al. 2015

Inflammation and Regeneration

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Transplantation of neural cells is a promising therapeutic strategy for spinal cord injury (SCI). Here we generated neurons including those with spinal motoneuron phenotype from human induced pluripotent stem (hiPS) cells by leading to formation of embryoid bodies (EB) and subsequent adherent culture for 4 days during which retinoic acid (RA), noggin (NOG) and sonic hedgehog (SHH) were introduced twice. Spinal motoneuron specific HB9 mRNA expression remarkably increased in the presence of RA, NOG and SHH. With this culture condition, the neurons expressed neurofilament middle chain (NFM), βIII tubulin and HB9 proteins in vitro. We then transplanted the hiPS derived human neurons into SCI mice with complete transection of Th11. Motor function of neuron transplanted SCI mice was significantly improved compared with those of vehicle injected SCI mice. Grafted cells survived and expressed βIII tubulin and HB9 diffusely 36 days after the transplantation. Galactocerebroside positive cells increased and glial fibrillary acidic protein (GFAP) expression reduced in the transection site of neuron transplanted SCI mice. The grafted motoneurons elongated human neural cell adhesion molecule (hNCAM) positive axons over the L1 spinal level. NCAM positive axons at L1 carried a neuron tracer cholera toxin β subunit (CTβ), which had been injected into Th10 of spinal cord, cranial region of the SCI/motoneuron grafted site (Th11). Collectively we succeeded in generating neurons which include those with HB9 positive spinal motoneuron phenotype from hiPS cells. The neurons brought about restoration of the motor function and histological improvement in an SCI. Rec.1/23/2015, Acc.4/15/2015, pp154-163

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Section: )Cell Transplantationmentioning

confidence: 99%

Induction of neural cells with spinal motoneuron phenotype from human iPS cells and the transplantation to totally transected spinal cords in mice

Iinuma

Umehara

Arimitsu

et al. 2015

Inflammation and Regeneration

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“…Deletion of Ddb1 also led to a blockade of hepatocyte proliferation, which was partially due to accumulation of p21. It is also worth noting that thermoreversible gelation polymer, a synthetic material that polymerizes when exposed to temperatures above a defined threshold, induced ductular reactions when injected directly into normal rat liver (Nagaya, Kubota, Suzuki, Akashi, & Mitaka, 2006). This suggests that ECM can provide cues for activation of the HPC compartment.…”

Section: Conditions That Activate Hpc Proliferationmentioning

confidence: 99%

The Origin, Biology, and Therapeutic Potential of Facultative Adult Hepatic Progenitor Cells

Shin

Kaestner

2014

Current Topics in Developmental Biology

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The liver plays an essential role in glucose and lipid metabolism, synthesis of plasma proteins, and detoxification of xenobiotics and other toxins. Chronic disease of this important organ is one of the leading causes of death in the United States. Following loss of tissue, liver mass can be restored by two mechanisms. Under normal conditions, or after massive loss of parenchyma by surgical resection, liver mass is maintained by division of hepatocytes. After chronic injury, or when proliferation of hepatocytes is impaired, facultative adult hepatic progenitor cells (HPCs) proliferate and differentiate into hepatocytes and cholangiocytes (biliary epithelial cells). HPCs are attractive candidates for cell transplantation because of their potential contribution to liver regeneration. However, until recently, the lack of highly specific markers has hampered efforts to better understand the origin and physiology of HPCs. Recent advances in cell isolation methods and genetic lineage tracing have enabled investigators to explore multiple aspects of HPC biology. In this review, we describe the potential origins of HPCs, the markers used to detect them, the contribution of HPCs to recovery, and the signaling pathways that regulate their biology. We end with an examination of the therapeutic potential of HPCs and their derivatives.

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“…Nagaya et al reported the use of TGP for regeneration of focal liver injury. 22 In their study, TGP induced efficient regeneration of hepatocytes by reducing the fibrosis and enhancing proliferation. Sudha et al showed the growth of human cadaver corneal limbal epithelial cells cultured in TGP, and they showed that the cells expressed both the limbal and corneal phenotype.…”

Section: Discussionmentioning

confidence: 96%

“…Nagaya et al . reported the use of TGP for regeneration of focal liver injury . In their study, TGP induced efficient regeneration of hepatocytes by reducing the fibrosis and enhancing proliferation.…”

Section: Discussionmentioning

confidence: 99%

Buccal epithelium Expanded and Encapsulated in Scaffold‐Hybrid Approach to Urethral Stricture (BEES‐HAUS) procedure: A novel cell therapy‐based pilot study

2018

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Objectives To describe the feasibility of a novel cell‐based endoscopic technique using buccal epithelium, expanded and encapsulated in a thermoreversible gelation polymer scaffold for the treatment of urethral stricture. Methods Six male patients with bulbar urethral stricture ranging from 2.0 to 3.5 cm in length were included in this pilot study. Autologous buccal epithelial cells from a small buccal mucosal biopsy were isolated, cultured and encapsulated in thermoreversible gelation polymer scaffold, and were implanted at the stricture site after a wide endoscopic urethrotomy. Results All the patients voided well, with a mean peak flow rate of 24 mL/s. Urethroscopy carried out at 6 months showed healthy mucosa at the urethrotomy site. However, two of the six patients had recurrence at 18 and 24 months, respectively. Conclusions This endoscopic‐based Buccal epithelium Expanded and Encapsulated in Scaffold‐Hybrid Approach to Urethral Stricture (BEES‐HAUS) technique is a promising alternative for the open substitution buccal graft urethroplasty. It is possible to achieve the benefits of open substitution buccal urethroplasty with this endoscopic technique.

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Thermoreversible gelation polymer induces the emergence of hepatic stem cells in the partially injured rat liver

Cited by 17 publications

References 17 publications

Induction of neural cells with spinal motoneuron phenotype from human iPS cells and the transplantation to totally transected spinal cords in mice

Induction of neural cells with spinal motoneuron phenotype from human iPS cells and the transplantation to totally transected spinal cords in mice

The Origin, Biology, and Therapeutic Potential of Facultative Adult Hepatic Progenitor Cells

Buccal epithelium Expanded and Encapsulated in Scaffold‐Hybrid Approach to Urethral Stricture (BEES‐HAUS) procedure: A novel cell therapy‐based pilot study

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