Thermoresponsive polymers as gene delivery vectors: Cell viability, DNA transport and transfection studies

Twaites, Beverley R.; Alarcón, Carolina de las Heras; Lavigne, Matthieu D.; Saulnier, A.; Pennadam, Sivanand S.; Cunliffe, David; Górecki, Dariusz C.; Alexander, Cameron

doi:10.1016/j.jconrel.2005.08.009

Cited by 93 publications

(71 citation statements)

References 36 publications

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“…Most of the studies discussed in this review use polymers that present an LCST. The most common of these is poly(N-isopropylacrylamide) (PNIPAAm) [1][2][3][4]. PNIPAAm has a lower critical solution temperature, LCST of around 32 °C, a very useful temperature for biomedical applications since it is close to the body temperature (37 °C).…”

Section: Open Accessmentioning

confidence: 99%

“…"Smart" materials, i.e., materials that have the ability to respond to external stimuli, represent one of the most exciting and immerging class of materials [1][2][3][4][5][6][7][8][9]. One of the main groups of "smart" materials is based on polymers because they are cheaper and more easily tailored than metals or ceramics.…”

Section: Introductionmentioning

confidence: 99%

“…One of the main groups of "smart" materials is based on polymers because they are cheaper and more easily tailored than metals or ceramics. These polymeric "smart" materials can respond to stimuli such as pH, temperature, ionic strength, electric or magnetic field, light and/or chemical and biological stimuli and consequently have a wide range of applications that include sensors, drug delivery, gene delivery and tissue engineering [1][2][3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

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Thermoresponsive Polymers for Biomedical Applications

2011

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Thermoresponsive polymers are a class of "smart" materials that have the ability to respond to a change in temperature; a property that makes them useful materials in a wide range of applications and consequently attracts much scientific interest. This review focuses mainly on the studies published over the last 10 years on the synthesis and use of thermoresponsive polymers for biomedical applications including drug delivery, tissue engineering and gene delivery. A summary of the main applications is given following the different studies on thermoresponsive polymers which are categorized based on their 3-dimensional structure; hydrogels, interpenetrating networks, micelles, crosslinked micelles, polymersomes, films and particles.

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Section: Open Accessmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Thermoresponsive Polymers for Biomedical Applications

2011

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“…In the literature, it has been discussed that extreme results in the MTT assay (any material inducing a reduction of > 30% in cell viability (cytotoxicity assay according to ISO 10993-5) is considered toxic) suggests that the tested material is toxic and should be further investigated with other methods [54,55]. It is also tempting to speculate that MSCs' interaction with these modified hydrogels may cause them to increase their potency and may increase their differentiation capacity.…”

Section: Modified Filmsmentioning

confidence: 99%

Poly (hydroxyethyl methacrylate-glycidyl methacrylate) films modified with different functional groups: In vitro interactions with platelets and rat stem cells

Bayramoğlu

Bitirim

Tunalı

et al. 2013

Materials Science and Engineering: C

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Keywords: Hydrogels biocompatible polymers contact angle blood cell adhesion hemolysis rat mesenchymal stem cells Copolymerization of 2-hydroxyethylmethacrylate (HEMA) with glycidylmethacrylate (GMA) in the presence of α-α′-azoisobisbutyronitrile (AIBN) resulted in the formation of hydrogel films carrying reactive epoxy groups. Thirteen kinds of different molecules with pendant \NH 2 group were used for modifications of the p(HEMA-GMA) films. The \NH 2 group served as anchor binding site for immobilization of functional groups on the hydrogel film via direct epoxy ring opening reaction. The modified hydrogel films were characterized by FTIR, and contact angle studies. In addition, mechanical properties of the hydrogel films were studied, and modified hydrogel films showed improved mechanical properties compared with the non-modified film, but they are less elastic than the non-modified film. The biological activities of these films such as platelet adhesion, red blood cells hemolysis, and swelling behavior were studied. The effect of modified hydrogel films, including \NH 2 , (using different aliphatic \CH 2 chain lengths) \CH 3 , \SO 3 H, aromatic groups with substituted \OH and \COOH groups, and amino acids were also investigated on the adhesion, morphology and survival of rat mesenchymal stem cells (MSCs). The MTT colorimetric assay reveals that the p(HEMA-GMA)-GA-AB, p(HEMA-GMA)-GA-Phe, p(HEMA-GMA)-GA-Trp, p(HEMA-GMA)-GA-Glu formulations have an excellent biocompatibility to promote the cell adhesion and growth. We anticipate that the fabricated p(HEMA-GMA) based hydrogel films with controllable surface chemistry and good stable swelling ratio may find extensive applications in future development of tissue engineering scaffold materials, and in various biotechnological areas.

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“…It is important to note that the reading of control group on MTT assay was obtained from cells on 96-well culture plates without CNT arrays. Previously, it was shown that less than 40% cell viability at the MTT results suggested that the tested material is toxic [37] and should be further investigated with other methods. Our data in Fig.…”

Section: Seeding Mscs On the Patterned Cnt Arraysmentioning

confidence: 99%

Patterned carbon nanotubes as a new three-dimensional scaffold for mesenchymal stem cells

Bitirim

Kucukayan-Dogu

Bengu

et al. 2013

Materials Science and Engineering: C

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We investigated the cellular adhesive features of mesenchymal stem cells (MSC) on non-coated and collagen coated patterned and vertically aligned carbon nanotube (CNT) structures mimicking the natural extra cellular matrix (ECM). Patterning was achieved using the elasto-capillary induced by water treatment on the CNT arrays. After confirmation with specific markers both at transcript and protein levels, MSCs from different passages were seeded on either collagen coated or non-coated patterned CNTs. Adhesion and growth of MSCs on the patterned CNT arrays were examined using scanning electron microscopy image analysis and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide (MTT) assays. The highest MSC count was observed on the non-coated patterned CNTs at passage zero, while decreasing numbers of MSCs were found at the later passages. Similarly, MTT assay results also revealed a decrease in the viability of the MSCs for the later passages. Overall, the cell count and viability experiments indicated that MSCs were able to better attach to non-coated patterned CNTs compared to those coated with collagen. Therefore, the patterned CNT surfaces can be potentially used as a scaffold mimicking the ECM environment for MSC growth which presents an alternative approach to MSC-based transplantation therapy applications.

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Thermoresponsive polymers as gene delivery vectors: Cell viability, DNA transport and transfection studies

Cited by 93 publications

References 36 publications

Thermoresponsive Polymers for Biomedical Applications

Thermoresponsive Polymers for Biomedical Applications

Poly (hydroxyethyl methacrylate-glycidyl methacrylate) films modified with different functional groups: In vitro interactions with platelets and rat stem cells

Patterned carbon nanotubes as a new three-dimensional scaffold for mesenchymal stem cells

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