Thermoresponsive Microtextured Culture Surfaces Facilitate Fabrication of Capillary Networks

Tsuda, Yukiko; Yamato, Masayuki; Kikuchi, Akihiko; Watanabe, Masanao; Chen, Guoping; Takahashi, Yoshihiro; Okano, Teruo

doi:10.1002/adma.200700988

Cited by 27 publications

(29 citation statements)

References 26 publications

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“…By imprinting the capillary pattern in a thermoresponsive polymer it is possible to harvest an EC tubular network that can then be used in the fabrication of 3D vascularized tissue grafts. [111] Unfortunately, a drawback of photolithography is its inability to create 3D architectures. Recently, a novel computer-aided design/computer-aided manufacturing (CAD/CAM) technology named two-photon polymerization (2PP) has emerged that allows the fabrication of any computer-designed 3D structure with a structural resolution down to 100 nm from a photosensitive polymer material.…”

Section: Microfabrication Of Network With Vascular Geometrymentioning

confidence: 99%

Vascularization in Bone Tissue Engineering: Physiology, Current Strategies, Major Hurdles and Future Challenges

Santos

Reis

2009

Macromolecular Bioscience

383

273

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The lack of a functional vascular supply has, to a large extent, hampered the whole range of clinical applications of 'successful' laboratory-based bone tissue engineering strategies. To the present, grafts have been dependent on post-implant vascularization, which jeopardizes graft integration and often leads to its failure. For this reason, the development of strategies that could effectively induce the establishment of a microcirculation in the engineered constructs has become a major goal for the tissue engineering research community. This review addresses the role and importance of the development of a vascular network in bone tissue engineering and provides an overview of the most up to date research efforts to develop such a network.

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Section: Microfabrication Of Network With Vascular Geometrymentioning

confidence: 99%

Vascularization in Bone Tissue Engineering: Physiology, Current Strategies, Major Hurdles and Future Challenges

Santos

Reis

2009

Macromolecular Bioscience

383

273

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“…Using soft lithography to fabricate micropatterned poly(urethane acrylate) substrates with varying ridge widths of 5, 10, 20, and 30 mm separated by 20 mm grooves, we created microtextured temperature-responsive culture surfaces by covalent grafting and polymerization with PIPAAm. [21] With this method, PIPAAm was grafted onto both the ridges and grooves of the poly(urethane acrylate) substrates. However, due to the increased thickness of the grafted PIPAAm in the grooves, which prevented cell adhesion, the seeded endothelial cells migrated out of the grooves and specifically adhered only to the ridge surfaces.…”

Section: Fabrication Of Tubular Endothelial-cell Network Using Pattementioning

confidence: 99%

Tissue Engineering Using Laminar Cellular Assemblies

et al. 2009

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As proposed in the late 1980s by Langer and Vacanti, the ultimate goal of tissue engineering is the development of structures that can be used to treat or replace damaged or diseased organs and tissues. For the regeneration of various organs such as the heart, liver, and kidney, the development of adequate vascular networks within the engineered tissues remains a significant obstacle in the formation of cell‐dense structures that resemble the native parenchyma. While tissue engineering using biodegradable scaffolds has been successful in the re‐creation of tissues where extracellular matrix is abundant, we have developed cell‐sheet‐based tissue engineering for the construction of tissues using laminar assemblies of cells harvested from temperature‐responsive culture dishes. Using cell sheet engineering, we present new strategies for the development of organ‐like tissue structures containing well‐organized vascular networks.

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“…[6][7][8][9] Thermo-responsive polymer brushes have been considered as functional substrate modifiers as their surface wettability can be easily adjusted by changing temperature, enabling application in controlled cell adhesion and detachment. 10,11 The most widely studied thermoresponsive polymer is poly(N-isopropylacrylamide) (PNiPAm), which exhibits a lower critical solution temperature (LCST) phase transition in water at 32 ⁰C. This polymer has been prepared in the form of surface-displayed brushes which can be used to facilitate cell sheet generation by incubating cells at the polymer brush surface at physiological temperatures, followed by recovery of cells in increased numbers after a culture stage and subsequent cooling of the surfaces below the polymer phase transition temperature to enable cell detachment and recovery.…”

Section: Introductionmentioning

confidence: 99%

Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment

et al. 2017

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We report the synthesis of thermo-responsive polymer brushes with Upper Critical Solution Temperature (UCST)-type behaviour on glass to provide a new means to control cell attachment. Thermoresponsive poly(N-acryloyl glycinamide)-statpoly(N-phenylacrylamide) (PNAGAm-PNPhAm) brushes with three different monomer ratios were synthesized to give tunable phase transition temperatures (Tp) in solution. Surface energies of surface-grafted brushes of these polymers at 25, 32, 37 and 50 C were calculated from contact angle measurements and atomic force microscopy (AFM) studies confirmed that these polymers were highly extended at temperatures close to Tp in physiologically-relevant media. Importantly, NIH-3T3 cells were attached on the collapsed PNAGAm-PNPhAm brush surface at 30 C after 20 h incubation, while release of cells from the extended brushes was observed within 2 h after the culture temperature was switched to 37 C. Furthermore, the changes in cell attachment followed changes in the Lewis base component of surface energy. The results indicate that, in contrast to the established paradigm of enhanced cell attachment to surfaces where polymers are above a Lower Critical Solution Temperature (LCST), these novel substrates enable detachment of cells from surfaces at temperatures above a UCST. In turn these responsive materials open new avenues for the use of polymer-modified surfaces to control cell attachment for applications in cell manufacture and regenerative medicine.

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Thermoresponsive Microtextured Culture Surfaces Facilitate Fabrication of Capillary Networks

Cited by 27 publications

References 26 publications

Vascularization in Bone Tissue Engineering: Physiology, Current Strategies, Major Hurdles and Future Challenges

Vascularization in Bone Tissue Engineering: Physiology, Current Strategies, Major Hurdles and Future Challenges

Tissue Engineering Using Laminar Cellular Assemblies

Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment

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