2019
DOI: 10.1021/acsbiomaterials.9b00082
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Thermoresponsive Injectable Microparticle–Gel Composites with Recombinant BMP-9 and VEGF Enhance Bone Formation in Rats

Abstract: Bone morphogenetic protein-9 (BMP-9) has been shown to be the most osteogenic BMP. Most of these experiments, however, involve an adenovirus-transfection strategy. Here, we used the scaffold-based strategy to study the bone forming ability of recombinant BMP-9 combined with vascular endothelial growth factor (VEGF). A robust, injectable, multicomponent-releasing scaffold in the form of a composite gel was developed by combining chitosan microparticles (MPs) with thermosensitive gel (MPs-gel). The MPs acted as … Show more

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Cited by 20 publications
(10 citation statements)
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References 48 publications
(112 reference statements)
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“…All experiments were done in triplicate and mean cell viabilities were calculated against the negative control (cells only). 58 …”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…All experiments were done in triplicate and mean cell viabilities were calculated against the negative control (cells only). 58 …”
Section: Methodsmentioning
confidence: 99%
“…All experiments were done in triplicate and mean cell viabilities were calculated against the negative control (cells only). 58 In vitro lung deposition test. Twin glass impinge set (Copley Scientic Limited, Nottingham, UK) was used to assess aerodynamic behavior of the nal particle (APCL-ZnO-CLOX) which showed the highest biocompatibility.…”
Section: S1 In Esi †)mentioning
confidence: 99%
“…This growth factor is widely used in bone tissue engineering. The co-delivery of VEGF and BMP2 for synergetic effects has been the topic of some research works [ 71 , 72 ]. In a study [ 73 ], PLGA microcarriers, prepared using microfluidics and loaded with VEGF, had their surface modified with poly(3,4-dihy-droxyphenethylamine) (PDA) for BMP2 conjugation.…”
Section: Fields Of Applicationmentioning
confidence: 99%
“…Alternatively, a salt could be added to the gel to lower the gelation temperature, however, studies have shown a consequent decrease in the strength of such gels when in an aqueous solution or in a cell culture environment. To circumvent these issues and formulate a methylcellulose gel capable of thermogelation at physiological body temperature, Gaihre et al (2019) recently blended calcium chloride and alginate with methylcellulose, resulting in the formation of a crosslinked, strong gel within 10 min at 37 • C. The resulting formulation served as an injectable medium for the delivery of chitosan microparticles. Furthermore, faster gelation was observed upon the addition of the chitosan microparticles due to the absorption of water from the solution by the microparticles, thereby increasing the viscosity of the solution, as was evidenced by the swelling of the microparticles when added into the solution (Gaihre et al, 2019).…”
Section: Cellulose Based Thermogelsmentioning
confidence: 99%