Thermoresponsive hydrogels from BSA esterified with low molecular weight PEG

Wang, Kai; Buschle‐Diller, Gisela; Wu, Yufeng

doi:10.1002/app.40946

Cited by 11 publications

(8 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown in Figure 1 B, BSA-based hydrogels were chosen as the model protein hydrogel due to their stable mechanical properties and excellent biocompatibility [ 33 , 34 , 35 , 36 , 37 ]. By mixing BSA protein solutions with N-hydroxysuccinimide-terminated 4-armed PEG (Mw: 20 kDa, named PEG), transparent hydrogels (denoted as BSA-PEG hydrogels) formed immediately in several minutes.…”

Section: Resultsmentioning

confidence: 99%

Tuning Strain Stiffening of Protein Hydrogels by Charge Modification

Guo

et al. 2022

IJMS

View full text Add to dashboard Cite

Strain-stiffening properties derived from biological tissue have been widely observed in biological hydrogels and are essential in mimicking natural tissues. Although strain-stiffening has been studied in various protein-based hydrogels, effective approaches for tuning the strain-stiffening properties of protein hydrogels have rarely been explored. Here, we demonstrated a new method to tune the strain-stiffening amplitudes of protein hydrogels. By adjusting the surface charge of proteins inside the hydrogel using negatively/positively charged molecules, the strain-stiffening amplitudes could be quantitively regulated. The strain-stiffening of the protein hydrogels could even be enhanced 5-fold under high deformations, while the bulk property, recovery ability and biocompatibility remained almost unchanged. The tuning of strain-stiffening amplitudes using different molecules or in different protein hydrogels was further proved to be feasible. We anticipate that surface charge adjustment of proteins in hydrogels represents a general principle to tune the strain-stiffening property and can find wide applications in regulating the mechanical behaviors of protein-based hydrogels.

show abstract

Section: Resultsmentioning

confidence: 99%

Tuning Strain Stiffening of Protein Hydrogels by Charge Modification

Guo

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…As described in a previous work, UV‐vis spectroscopy was used (Thermo Scientific Genesys 6 spectrometer; Thermo Scientific, Waltham, MA; quartz cuvettes with 10 mm path length) to measure the yield and concentration of the BSA moiety before the coating of the samples. The absorption coefficient for modified BSA used was 3,443,824 M −1 cm −1 (280 nm).…”

Section: Methodsmentioning

confidence: 99%

Scaffold materials from glycosylated and PEGylated bovine serum albumin

Wang

David

Choi

et al. 2015

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

Bovine serum albumin has been PEGylated and glycosylated to create mimetic materials for the extracellular matrix (ECM) with potential tissue engineering applications. Different surfaces for cell adhesion were achieved by crosslinking the initial albumin product and forming either a coating or a sponge-like three-dimensional morphology to mimic the mesh structure of natural ECM. The biocompatibility of the albumin matrix with mammalian cells was evaluated using cell culture assays with NIH 3T3 cells. The results indicated that glycoprotein composition and specific morphology of the assembly can improve the cell growth environment. These ECM mimetic structures might eventually be considered to serve as alternatives for the more expensive collagen and elastin based ECM substances currently in use in tissue engineering.

show abstract

“…Albumin, which is a natural globular protein commonly found in blood plasma, is capable of showing a stimuli-responsive property in response to external stimuli like temperature and pH [24]. The thermo-responsive hydrogel made from bovine serum albumin (BSA) and low molecular weight polyethylene glycol (PEG) showed sol–gel transition near the body temperature and it was reported to be a promising drug delivery material [78]. The pH responsive hydrogel developed by free radical polymerization of methacrylate bovine serum albumin and N -isopropylacrylamide was used as a drug delivery system for caffeine and theophylline [79].…”

Section: Natural Stimuli-responsive Polymersmentioning

confidence: 99%

Review of Stimuli-Responsive Polymers in Drug Delivery and Textile Application

2019

View full text Add to dashboard Cite

This review describes some commercially available stimuli-responsive polymers of natural and synthetic origin, and their applications in drug delivery and textiles. The polymers of natural origin such as chitosan, cellulose, albumin, and gelatin are found to show both thermo-responsive and pH-responsive properties and these features of the biopolymers impart sensitivity to act differently under different temperatures and pH conditions. The stimuli-responsive characters of these natural polymers have been discussed in the review, and their respective applications in drug delivery and textile especially for textile-based transdermal therapy have been emphasized. Some practically important thermo-responsive polymers such as pluronic F127 (PF127) and poly(N-isopropylacrylamide) (pNIPAAm) of synthetic origin have been discussed in the review and they are of great importance commercially because of their in situ gel formation capacity. Some pH-responsive synthetic polymers have been discussed depending on their surface charge, and their drug delivery and textile applications have been discussed in this review. The selected stimuli-responsive polymers of synthetic origin are commercially available. Above all, the applications of bio-based or synthetic stimuli-responsive polymers in textile-based transdermal therapy are given special regard apart from their general drug delivery applications. A special insight has been given for stimuli-responsive hydrogel drug delivery systems for textile-based transdermal therapy, which is critical for the treatment of skin disease atopic dermatitis.

show abstract

Thermoresponsive hydrogels from BSA esterified with low molecular weight PEG

Cited by 11 publications

References 40 publications

Tuning Strain Stiffening of Protein Hydrogels by Charge Modification

Tuning Strain Stiffening of Protein Hydrogels by Charge Modification

Scaffold materials from glycosylated and PEGylated bovine serum albumin

Review of Stimuli-Responsive Polymers in Drug Delivery and Textile Application

Contact Info

Product

Resources

About