Thermoresponsive hyaluronic acid nanogels as hydrophobic drug carrier to macrophages

Stefanello, Talitha Fernandes; Szarpak‐Jankowska, Anna; Appaix, Florence; Louage, Benoit; Hamard, Lauriane; Geest, Bruno G. De; Sanden, Boudewijn van der; Nakamura, Celso Vataru; Auzély‐Velty, Rachel

doi:10.1016/j.actbio.2014.07.033

Cited by 51 publications

(30 citation statements)

References 29 publications

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“…Systemic delivery of miRNA as well as other nucleic acids to macrophages is very challenging due to their highly degradative phagocytic, endosomal, and lysosomal compartments and unstable properties of nucleic acids [ 20 – 22 ]. In this study, HA-PEI conjugate was designed for delivery of miR-223 duplex to macrophages due to the high capacity of PEI for encapsulation of nucleic acids and targeting ability of HA to CD44-overexpressed cancer cells and macrophages [ 13 , 23 , 24 ]. As shown in Fig 1 , the conjugate had high capacity for miR-223 encapsulation due to the abundant amine groups of PEI to condense miR-223 by electrostatic interactions.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-223 Induced Repolarization of Peritoneal Macrophages Using CD44 Targeting Hyaluronic Acid Nanoparticles for Anti-Inflammatory Effects

2016

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The aim of this study was to evaluate macrophages repolarization from pro-inflammatory M1 to anti-inflammatory M2 phenotype upon transfection with microRNA-223 (miR-223) duplexes and miR-223 expressing plasmid DNA encapsulated in CD44-targeting hyaluronic acid-poly(ethyleneimine) (HA-PEI) nanoparticles (NPs). The HA-PEI/miR-223 NPs with spherical shape and an average diameter of 200 nm were efficiently internalized by J774A.1 alveolar and primary peritoneal macrophages and non-cytotoxic at HA-PEI concentration less than 200 μg/mL. Transfection of HA-PEI/miR-223 NPs in J774A.1 macrophages showed significantly higher miR-223 expression than that with HA-PEI/plasmid DNA expressing miR-223 (pDNA-miR-223). HA-PEI/miR-223 NPs mediated transfection increased miR-223 expression to 90 fold in primary peritoneal macrophages compared to untreated cells. The overexpression of miR-223 in both J774A.1 and peritoneal macrophages induced a phenotypic change from M1 to M2 state as indicated by a decrease in iNOS-2 (M1 marker) and an increase in Arg-1 (M2 marker) levels compared to those in lipopolysaccharide (LPS) and interferon-gamma (IFN-γ)-stimulated macrophages (M1). The change in macrophage phenotype by HA-PEI/miR-223 NPs could suppress the inflammation in peritoneal macrophages induced by LPS as evidenced by a significant decrease in pro-inflammatory cytokine levels TNF-α, IL-1β and IL-6, compared to LPS-stimulated peritoneal macrophages without treatment. The results demonstrated that miR-223-encapsulated HA-PEI NPs modulated macrophage polarity toward an anti-inflammatory M2 phenotype, which has potential for the treatment of inflammatory diseases.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-223 Induced Repolarization of Peritoneal Macrophages Using CD44 Targeting Hyaluronic Acid Nanoparticles for Anti-Inflammatory Effects

2016

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“…In recent years, natural anionic polysaccharide hyaluronic acid (HA)-based nanoparticles have emerged as promising nanocarriers for targeting drugs and genes to tumor cells which over-express CD44, a receptor of HA 17 18 19 20 . HA-based nanogels and HA-modified liposomes have also been used for targeting CD44-overexpressed macrophages 21 22 . For gene delivery, HA has been modified with a cationic polymer which has capacity of forming complex with negatively charged nucleic acids 17 .…”

mentioning

confidence: 99%

Modulation of Macrophage Functional Polarity towards Anti-Inflammatory Phenotype with Plasmid DNA Delivery in CD44 Targeting Hyaluronic Acid Nanoparticles

Trần

Rastogi

Shelke

et al. 2015

Sci Rep

101

105

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The purpose of this study was to modulate macrophage polarity from the pro-inflammatory M1 to anti-inflammatory M2 phenotype using plasmid DNA (pDNA) expressing interleukin-4 (IL4) or interleukin-10 (IL10)-encapsulated in hyaluronic acid-poly(ethyleneimine) (HA-PEI) nanoparticles (NPs). The HA-PEI/pDNA NPs with spherical shape, average size of 186 nm were efficiently internalized by J774A.1 macrophages. Transfection of HA-PEI/pDNA-IL4 and HA-PEI/pDNA-IL10 NPs increased IL4 and IL10 gene expression in J774 macrophages which could re-program the macrophages from M1 to M2 phenotype as evidenced by a significant increase in the Arg/iNOS level, and upregulation of CD206 and CD163 compared to untreated macrophages. Following intraperitoneal (IP) injection to C57BL/6 mice, HA-PEI NPs effectively targeted peritoneal macrophages over-expressing CD44 receptor. In an in vivo model of stimulated peritoneal macrophages, IP administration of HA-PEI/pDNA-IL4 and HA-PEI/pDNA-IL10 to C57BL/6 mice significantly increased the Arg/iNOS ratio and CD163 expression in the cells. Furthermore, HA-PEI/pDNA-IL10 NPs significantly increased peritoneal and serum IL10 levels which effectively suppressed LPS-induced inflammation by reducing level of TNF-α and IL-1β in peritoneal macrophages and in the peritoneal fluid. The results demonstrated that pDNA-IL10-encapsulate HA-PEI NPs skewed macrophage functional polarity from M1 toward an anti-inflammatory M2 phenotype which may be a promising platform for the treatment of inflammatory diseases.

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“…After intravenous injection of nanogels, mice can be quickly engulfed by circulating macrophages within 13 min and stored in the liver and spleen. The study also showed that HA-CD44 binding can effectively attach nanocarriers to macrophages (Fernandes Stefanello et al., 2014 ).…”

Section: Encapsulation/conjugation Of Drugs/particles To Cellsmentioning

confidence: 96%

Cell-mediated targeting drugs delivery systems

Yang

et al. 2020

Drug Delivery

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Drug delivery systems have shown tremendous promise to improve the diagnostic and therapeutic effects of drugs due to their special property. Targeting tissue damage, tumors, or drugs with limited toxicity at the site of infection is the goal of successful pharmaceuticals. Targeted drug delivery has become significantly important in enhancing the pharmaceutical effects of drugs and reducing their side effects of therapeutics in the treatment of various disease conditions. Unfortunately, clinical translation of these targeted drug delivery system mechanisms faces many challenges. At present, only a few targeted drug delivery systems can achieve high targeting efficiency after intravenous injection, even though numerous surface markers and targeting approaches have been developed. Thus, cellmediated drug-delivery targeting systems have received considerable attention for their enhanced therapeutic specificity and efficacy in the treatment of the disease. This review highlights the recent advances in the design of the different types of cells that have been explored for cell-mediated drug delivery and targeting mechanisms. A better understanding of cell biology orientation and a new generation of delivery strategies that utilize these endogenous approaches are expected to provide better solutions for specific site delivery and further facilitate clinical translation.

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Thermoresponsive hyaluronic acid nanogels as hydrophobic drug carrier to macrophages

Cited by 51 publications

References 29 publications

MicroRNA-223 Induced Repolarization of Peritoneal Macrophages Using CD44 Targeting Hyaluronic Acid Nanoparticles for Anti-Inflammatory Effects

MicroRNA-223 Induced Repolarization of Peritoneal Macrophages Using CD44 Targeting Hyaluronic Acid Nanoparticles for Anti-Inflammatory Effects

Modulation of Macrophage Functional Polarity towards Anti-Inflammatory Phenotype with Plasmid DNA Delivery in CD44 Targeting Hyaluronic Acid Nanoparticles

Cell-mediated targeting drugs delivery systems

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