Thermoresponsive Epitope Surface-Imprinted Nanoparticles for Specific Capture and Release of Target Protein from Human Plasma

Li, Senwu; Yang, Kaiguang; Deng, Nan; Min, Yi; Liu, Lukuan; Zhang, Lihua; Zhang, Yukui

doi:10.1021/acsami.5b11415

Cited by 70 publications

(43 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The lower functional monomers and higher amount of crosslinker led to the formation of the optimized thin layer from the monomeric mixture of MIP composition (Figure S4, Supporting Information), resulting in higher selectivity . The results indicated the MIP4 has the optimum functional monomers, crosslinker and template molar ratio of 158:88:1 (M:C:T) and consequently minimum nonspecific binding after the polymerization process …”

Section: Resultsmentioning

confidence: 99%

Efficient adsorptive extraction materials by surface protein‐imprinted polymer over silica gel for selective recognition/separation of human serum albumin from urine

Madhumanchi

Jadda

Suedee

2018

J of Applied Polymer Sci

View full text Add to dashboard Cite

In this study, we report the development of adsorptive extraction materials by surface protein‐imprinted polymers (MIPs) over silica gel for selective recognition/separation of human serum albumin (HSA) from urine. The HSA‐imprinted polymers prepared on silica particle had at interface between the silica gel and different MIPs greatly produced enrichment for the binding of protein from the urine. The solid‐phase extraction of the optimized polymer layer was prepared by copolymerization of methacrylic acid (MAA), acrylamide (AAm), and dimethylaminoethylmethacrylate (DMAEMA) and a crosslinker methylenebisacrylamide (MBA) at the mole ratio of 1:158:88 (T:M:C) and showed moderate affinity (<104 order M−1) toward target protein HSA and selectivity. Four analogues, egg white albumin (EWA), bovine serum albumin (BSA), lysozyme (Lyz), and creatinine (Cre) were selected to study the binding efficiency of MIPs in single and binary protein solutions. We studied the influence on recognition ability for HSA and found that prepolymer mixture and matrix flexibility of the optimized thin polymer layer (35 ± 10 nm) on the submicrosilica particles. The high‐binding affinity (QMIP, 86.7 mg g−1) and fast kinetics (180 min) were observed for this synthesized HSA‐MIP when compared with other reported HSA‐MIPs in surface imprinting (5.9 and 11.3 mg g−1) and epitope surface imprinting (46.6 mg g−1) methods. We demonstrated the application in real and synthetic urine samples that the approach allowed the efficient adsorption of HSA in real urine (129.48 mg g−1) is almost double to the binding of HSA in synthetic urine (67.84 mg g−1). Apart from this, only minor interference of Cre (2.74 mg g−1) was observed, eventhough Cre is the final metabolite in urine. These adsorptive submicrosilica materials have potential in the pharmaceutical industry and clinical analysis applications. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 46894.

show abstract

Section: Resultsmentioning

confidence: 99%

Efficient adsorptive extraction materials by surface protein‐imprinted polymer over silica gel for selective recognition/separation of human serum albumin from urine

Madhumanchi

Jadda

Suedee

2018

J of Applied Polymer Sci

View full text Add to dashboard Cite

show abstract

“…In order to resolve the recognition problem, Rachkov and Minoura suggested imprinting only fixed limited areas of recognition sites [75]. Epitope imprinting and a temperature- dependent capture and release process were performed by Li and coworkers [76]. In their study, SiO 2 nanoparticles were immobilized by glycidoxypropyltrimethoxysilane-iminodiacetic acid and 3-(trimethoxysilyl)propyl methacrylate to promote the template modification, as reflected in Figure 4.…”

Section: Developments In Macromolecular Imprinting Methodsmentioning

confidence: 99%

Molecular Imprinting of Macromolecules for Sensor Applications

Saylan

Yılmaz

Özgür

et al. 2017

Sensors

146

View full text Add to dashboard Cite

Molecular recognition has an important role in numerous living systems. One of the most important molecular recognition methods is molecular imprinting, which allows host compounds to recognize and detect several molecules rapidly, sensitively and selectively. Compared to natural systems, molecular imprinting methods have some important features such as low cost, robustness, high recognition ability and long term durability which allows molecularly imprinted polymers to be used in various biotechnological applications, such as chromatography, drug delivery, nanotechnology, and sensor technology. Sensors are important tools because of their ability to figure out a potentially large number of analytical difficulties in various areas with different macromolecular targets. Proteins, enzymes, nucleic acids, antibodies, viruses and cells are defined as macromolecules that have wide range of functions are very important. Thus, macromolecules detection has gained great attention in concerning the improvement in most of the studies. The applications of macromolecule imprinted sensors will have a spacious exploration according to the low cost, high specificity and stability. In this review, macromolecules for molecularly imprinted sensor applications are structured according to the definition of molecular imprinting methods, developments in macromolecular imprinting methods, macromolecular imprinted sensors, and conclusions and future perspectives. This chapter follows the latter strategies and focuses on the applications of macromolecular imprinted sensors. This allows discussion on how sensor strategy is brought to solve the macromolecules imprinting.

show abstract

“…The His‐tag has been the most widely used affinity tag for the efficient identification, production and isolation of recombinant proteins from crude biological extracts . Currently, immobilized metal ion affinity chromatograph (IMAC) has been applied as one of the most effective approaches to isolate His‐tagged proteins based on the metal coordination interaction between histidine and transition metal ions, usually Ni 2+ and Co 2+ . However, host proteins with histidine‐rich regions, inherent cysteines or metal centers can also be co‐purified non‐specifically by IMAC, which results in contamination and decreases purification selectivity.…”

Section: Applicationsmentioning

confidence: 99%

Epitope Imprinting Technology: Progress, Applications, and Perspectives toward Artificial Antibodies

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

Thermoresponsive Epitope Surface-Imprinted Nanoparticles for Specific Capture and Release of Target Protein from Human Plasma

Cited by 70 publications

References 32 publications

Efficient adsorptive extraction materials by surface protein‐imprinted polymer over silica gel for selective recognition/separation of human serum albumin from urine

Efficient adsorptive extraction materials by surface protein‐imprinted polymer over silica gel for selective recognition/separation of human serum albumin from urine

Molecular Imprinting of Macromolecules for Sensor Applications

Epitope Imprinting Technology: Progress, Applications, and Perspectives toward Artificial Antibodies

Contact Info

Product

Resources

About