Thermoresponsive dendronized chitosan-based hydrogels as injectable stem cell carriers

Zhang, Xiacong; Cheng, Lin; Feng, Letian; Yu, Peng; Zhou, Zhimin; Yin, Guoyong; Li, Wen; Zhang, Afang

doi:10.1039/c9py00256a

Cited by 23 publications

(36 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We seek to develop novel in situ-forming hydrogels with transparency and biocompatibility and can occur with the sol–gel transition triggered just by physiological temperature. In a previous work, we reported dendronized chitosans pendanted with 3-fold triethylene glycol monoethyl ether (TEG)-based dendron . However, they cannot show characteristic thermo-gelling behavior either in pure water or in PBS solution due to the relative high hydrophilicity of TEG dendrons, and chemical cross-linking through the Schiff-base reaction was needed to generate hydrogels.…”

Section: Results and Discussionmentioning

confidence: 99%

“…The structures of DCs were also confirmed by 1 H NMR spectroscopy, and a typical spectrum from DC 61 is shown in Figure B with characteristic peaks from CS (a and b) and G1 (c, e, f, g). G1 was attached to the CS backbone through the formation of covalent amide bond and noncovalent electronic interaction, as indicated by the appearance of two signals from the aromatic ring of G1 (d′ or d) …”

Section: Results and Discussionmentioning

confidence: 99%

“…We recently have developed a generalized methodology to confer various polymers with thermoresponsive properties through dendronization with 3-fold dendritic oligoethylene glycols (OEGs). − Chitosans (CS) are an intriguing class of natural polymers with excellent biocompatibility, antimicrobial, and anti-inflammatory properties. In the present work, through dendronization of chitosans with dendritic OEGs, we envisaged engineering a new type of thermoresponsive dendronized chitosans (DCs) as intelligent biomaterials for corneal tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Thermo-Gelling Dendronized Chitosans as Biomimetic Scaffolds for Corneal Tissue Engineering

Feng

Liu

Zhang

et al. 2021

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Biomimetic scaffolds with transparent, biocompatible, and in situ-forming properties are highly desirable for corneal tissue engineering, which can deeply fill corneal stromal defects with irregular shapes and support tissue regeneration. We here engineer a novel class of corneal scaffolds from oligoethylene glycol (OEG)-based dendronized chitosans (DCs), whose aqueous solutions show intriguing sol–gel transitions triggered by physiological temperature, resulting in highly transparent hydrogels. Gelling points of these hydrogels can be easily tuned, and furthermore, their mechanical strengths can be significantly enhanced when injected into PBS at 37 °C instead of pure water. In vitro tests indicate that these DC hydrogels exhibit excellent biocompatibility and can promote proliferation and migration of keratocyte. When applied in the rabbit eyes with corneal stromal defects, in situ formed DC hydrogels play a positive effect for new tissue regeneration. Overall, this thermo-gelling DCs possess appealing features as corneal tissue substitutes with their excellent biocompatibility and unprecedented thermoresponsiveness.

show abstract

Section: Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Thermo-Gelling Dendronized Chitosans as Biomimetic Scaffolds for Corneal Tissue Engineering

Feng

Liu

Zhang

et al. 2021

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…The 10% Cs-g-PNIPAAm exhibited the highest storage modulus when reconstituted in PBS (Figure 4b). The range of moduli at 37 • C reported in the literature for Cs-PNIPAAm hydrogels varied from approximately 1 Pa [71] to 1.4 kPa [72], depending on the formulation method. Although mechanical strength is not a prerequisite for this application, there should be sufficient strength to remain localized against in vivo biological forces that could disturb or dilute the injected hydrogel so as to provide the therapeutic effect for a required time [70,73].…”

Section: Discussionmentioning

confidence: 99%

Synthesis and Evaluation of a Thermoresponsive Degradable Chitosan-Grafted PNIPAAm Hydrogel as a “Smart” Gene Delivery System

et al. 2020

View full text Add to dashboard Cite

Thermoresponsive hydrogels demonstrate tremendous potential as sustained drug delivery systems. However, progress has been limited as formulation of a stable biodegradable thermosensitive hydrogel remains a significant challenge. In this study, free radical polymerization was exploited to formulate a biodegradable thermosensitive hydrogel characterized by sustained drug release. Highly deacetylated chitosan and N-isopropylacrylamide with distinctive physical properties were employed to achieve a stable, hydrogel network at body temperature. The percentage of chitosan was altered within the copolymer formulations and the subsequent physical properties were characterized using 1H-NMR, FTIR, and TGA. Viscoelastic, swelling, and degradation properties were also interrogated. The thermoresponsive hydrogels were loaded with RALA/pEGFP-N1 nanoparticles and release was examined. There was sustained release of nanoparticles over three weeks and, more importantly, the nucleic acid cargo remained functional and this was confirmed by successful transfection of the NCTC-929 fibroblast cell line. This tailored thermoresponsive hydrogel offers an option for sustained delivery of macromolecules over a prolonged considerable period.

show abstract

“…Recently, oligo(ethylene glycol) (OEG)-based dendronized polymers were reported to show unprecedented thermoresponsive behavior in water − and can provide confined microenvironments for encapsulation of guest molecules on an individual molecular level . These polymers were further cross-linked into macro- or nanogels through covalent or supramolecular chemistry with higher level confinement for various bioapplications. − Encouraged by these works, we herein exploited photochemistry to mediate intra- and intermolecular cross-linking of OEG-based dendronized polymers (Figure ). A cinnamate group was incorporated to the core of dendritic OEG units, and its dimerization can be easily controlled at intra- or intermolecular levels by UV irradiation below or above the phase transition temperature in water.…”

Section: Introductionmentioning

confidence: 99%

[2 + 2] Photocycloaddition-Mediated Intra- and Intermolecular Cross-Linking of Thermoresponsive Dendronized Polymethacrylates

Liu

Ren

et al. 2020

Macromolecules

Self Cite

View full text Add to dashboard Cite

Precise control on cross-linking of polymers is highly valuable to tune their structures and properties as well as for material applications. In this work, we present a robust methodology to mediate intra-and intermolecular cross-linking of thermoresponsive dendronized polymers through [2 + 2] photocycloaddition reactions. These polymers are constituted of polymethacrylates carrying a cinnamate-cored dendritic oligo(ethylene glycol) (OEG) pendant in each repeating unit. In dilute solutions, adjacent cinnamate moieties were efficiently dimerized by UV irradiation to afford intramolecularly cross-linked dendronized polymers. Their photocycloaddition behavior was followed by UV−vis and 1 H NMR spectroscopy, and the crowding effect from dendron dimerization on chain conformations was studied by SEC-LS and AFM. Interestingly, dendronized polymers retained single-chain cylinder topologies without branched and loop microstructures after photodimerization, and their hydrodynamic sizes in solutions kept nearly invariable, which is very different from the single-chain collapse of linear cinnamate polymers without OEG dendron pendants. These polymers exhibited characteristic thermoresponsive behavior in water, and their phase transition temperatures increase continuously with dimerization degrees. However, thermally induced interchain aggregations were surprisingly retarded during the phase transition, especially for polymers with high dimerization degrees. Furthermore, cross-linking of dendronized polymers was switched from intramolecular onto intermolecular fashion by photodimerization of the polymers above their phase transition temperatures in aqueous phases, which can be utilized to prepare thermoresponsive dendronized nanoparticles with uniform shapes and controllable sizes.

show abstract

Thermoresponsive dendronized chitosan-based hydrogels as injectable stem cell carriers

Abstract: A combination of dendronization and Schiff-base chemistry endows injectable chitosan hydrogels with thermoresponsiveness, self-healing abilities and enhanced mechanical properties under physiological conditions.

Cited by 23 publications

References 67 publications

Thermo-Gelling Dendronized Chitosans as Biomimetic Scaffolds for Corneal Tissue Engineering

Thermo-Gelling Dendronized Chitosans as Biomimetic Scaffolds for Corneal Tissue Engineering

Synthesis and Evaluation of a Thermoresponsive Degradable Chitosan-Grafted PNIPAAm Hydrogel as a “Smart” Gene Delivery System

[2 + 2] Photocycloaddition-Mediated Intra- and Intermolecular Cross-Linking of Thermoresponsive Dendronized Polymethacrylates

Contact Info

Product

Resources

About