Thermoprotective effect of insulin-like growth factor 1 on in vitro matured bovine oocyte exposed to heat shock

Rodrigues, T. A.; Ispada, J.; Risolia, P. H. B.; Rodrigues, Mariana T.; Lima, R. S.; Assumpção, M. E. O. A.; Visintin, José Antônio; Paula-Lopes, F. F.

doi:10.1016/j.theriogenology.2016.06.023

Cited by 28 publications

(26 citation statements)

References 72 publications

(88 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…At least part of the deleterious effects of heat shock on oocyte function is mediated by cellular and molecular damage to the oocyte. For example, heat shock reduced mitochondrial activity 36 , caused cytoskeleton disorganization 35 , 36 , protein denaturation, mRNA degradation as well as apoptosis 32 , 57 – 59 . In the present study, inhibition of autophagy prone the oocyte more susceptible to heat shock reducing the ability to cleave and reach the blastocyst stage.…”

Section: Discussionmentioning

confidence: 99%

“…This experiment was arranged in a 2 × 2 factorial design to determine the effect of temperature (38.5 or 41 °C) and autophagy inhibitor (0 or 10 mM 3MA) during IVM on autophagy activity by measuring LC3 protein level (autophagy marker). COCs were distributed on the following experimental groups: Control (38.5 °C for 22 h) and Heat Shock (41 °C for 16 h followed by 38.5 °C for 6 h 36 , 37 ), in the presence of 0 or 10 mM 3MA during IVM 38 . After IVM, COCs were denuded so that denuded oocytes and respective cumulus cells were processed for western blotting analysis to determine autophagy activity by LC3-I and LC3-II level.…”

Section: Methodsmentioning

confidence: 99%

“…A well-known stressor for oocytes is elevated temperature, to which bovine oocytes are often exposed leading to reduced fertility 29 , 30 . The deleterious effects of elevated temperature on bovine oocytes has been well characterized 30 – 36 . However, the molecular mechanisms involved in these processes are not fully understood.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Autophagy is a pro-survival adaptive response to heat shock in bovine cumulus-oocyte complexes

Latorraca

Feitosa

Mariano

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Autophagy is a physiological mechanism that can be activated under stress conditions. However, the role of autophagy during oocyte maturation has been poorly investigated. Therefore, this study characterized the role of autophagy on developmental competence and gene expression of bovine oocytes exposed to heat shock (HS). Cumulus-oocyte-complexes (COCs) were matured at Control (38.5 °C) and HS (41 °C) temperatures in the presence of 0 and 10 mM 3-methyladenine (3MA; autophagy inhibitor). Western blotting analysis revealed that HS increased autophagy marker LC3-II/LC3-I ratio in oocytes. However, there was no effect of temperature for oocytes matured with 3MA. On cumulus cells, 3MA reduced LC3-II/LC3-I ratio regardless of temperature. Inhibition of autophagy during IVM of heat-shocked oocytes (3MA-41 °C) reduced cleavage and blastocyst rates compared to standard in vitro matured heat-shocked oocytes (IVM-41 °C). Therefore, the magnitude of HS detrimental effects was greater in the presence of autophagy inhibitor. Oocyte maturation under 3MA-41 °C reduced mRnA abundance for genes related to energy metabolism (MTIF3), heat shock response (HSF1), and oocyte maturation (HAS2 and GREM1). In conclusion, autophagy is a stress response induced on heat shocked oocytes. inhibition of autophagy modulated key functional processes rendering the oocyte more susceptible to the deleterious effects of heat shock. Autophagy is a programmed lysosomal process that degrades damaged or unnecessary cellular proteins, lipids, DNA, RNA, and organelles for recycling amino acids, fatty acids, and nucleosides to act as cellular building blocks for anabolic processes 1-3. This event is involved in development, differentiation, immunity, aging, and cell death 2. The autophagy pathway can be triggered by different stress conditions in order to restore homeostasis as a prosurvival adaptive response to stress. For example, autophagy can be induced by diseases 4-6 and environmental factors such as nutrient deprivation 7,8 , high temperature 9-11 , oxidative stress 12,13 , hypoxia 14,15 , and toxins 16-18. There are three autophagy types described in mammalian cells: chaperone-mediated autophagy, macroautophagy, and microautophagy 1. In the chaperone-mediated autophagy, heat shock cognate 70 complex (Hsc70 complex) directs abnormal proteins that have KFERQ-like motifs exposed to lysosome-mediated degradation. On the other hand, during microautophagy the lysosome membrane invaginates, thus capturing a small portion of the cytoplasm to be degraded 2. Macroautophagy is the best-understood type of autophagy, which is mediated by double-membrane vesicles that transport abnormal proteins and organelles through the cytoplasm (i.e., autophagosomes). The autophagosome membrane fuses to lysosomes to breakdown the transported material 2. During embryonic development, autophagy is first induced after fertilization to clear sperm-born material that is not necessary for further embryogenesis 19-21. In turn, autophagy is also associated with abundance of maternal...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Autophagy is a pro-survival adaptive response to heat shock in bovine cumulus-oocyte complexes

Latorraca

Feitosa

Mariano

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cortical F‐actin was evaluated as described early (Rodrigues et al, 2016). Briefly, ImageJ version 1.43 was used to analyze the oocyte's digital images.…”

Section: Methodsmentioning

confidence: 99%

Endoplasmic reticulum distribution during bovine oocyte activation is regulated by protein kinase C via actin filaments

Feitosa

Lopes

Visintin

et al. 2020

Journal Cellular Physiology

View full text Add to dashboard Cite

Fertilization‐induced [Ca2+]i oscillations generally depend on the release of calcium ions from the endoplasmic reticulum (ER). Since ER is the main store of calcium ions, it plays an important role in oocyte fertilization. However, the mechanism of ER organization at oocyte activation is unknown. Here, we show that protein kinase C (PKC) is involved in ER distribution during bovine oocyte activation, but not involved in cell cycle resumption and spindle organization. Actin filaments were affected by PKC pharmacological inhibition. In addition, similar to PKC results, the actin‐depolymerizing drug cytochalasin B affected the ER distribution during oocyte activation. Specifically, we have demonstrated that ER organization during bovine oocyte activation is regulated by PKC possibly through its action on actin filaments regulation. Taken together, the results presented here provide further information on the pathway involved in the regulation of ER organization during oocyte activation and new insight into the functional role of PKC and actin filaments during this process.

show abstract

“…In pigs, although IGF-I has been reported to promote the synthesis of hyaluronic acid and the expansion of cumulus cells (Nemcova et al, 2007), its role in IVM remains unclear and has yield inconsistent results. Nevertheless, several studies reported that the addition of IGF-I during in vitro maturation and culture decreases apoptosis in bovine oocytes (Wasielak and Bogacki, 2007; Rodrigues et al, 2016; Ascari et al, 2017) and in porcine blastocysts (Wasielak et al, 2013). Wasielak et al (2013) also demonstrated that adding IGF-1 at a concentration of 100 ng/ml into maturation medium increases the BCL2L1:BAX transcript ratio in pig blastocysts, and the increased expression of anti-apoptotic genes, such as BCL2 , and a decreased expression of apoptotic-related genes, such as BAX , indicates higher blastocyst quality (Chen et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Supplementing Maturation Medium With Insulin Growth Factor I and Vitrification-Warming Solutions With Reduced Glutathione Enhances Survival Rates and Development Ability of in vitro Matured Vitrified-Warmed Pig Oocytes

et al. 2019

View full text Add to dashboard Cite

The present study sought to determine whether in vitro maturation (IVM) of pig oocytes in a medium supplemented with insulin growth factor-I (IGF-I) and subsequent vitrification with or without reduced glutathione (GSH) affect their quality and developmental competence, and the expression of genes involved in antioxidant, apoptotic and stress responses. In Experiment 1, cumulus-oocyte complexes were matured in the absence or presence of IGF-I (100 ng·mL−1) and then vitrified-warmed with or without 2 mM of GSH. Maturation rate was evaluated before vitrification, and oocyte viability, DNA fragmentation and relative transcript abundances of BCL-2-associated X protein (BAX), BCL2-like1 (BCL2L1), heat shock protein 70 (HSPA1A), glutathione peroxidase 1 (GPX1) and superoxide dismutase 1 (SOD1) genes were assessed in fresh and vitrified-warmed oocytes. In Experiment 2, fresh and vitrified-warmed oocytes were in vitro fertilized and their developmental competence determined. Whereas the addition of IGF-I to maturation medium had no effect on oocyte maturation, it caused an increase in the survival rate of vitrified-warmed oocytes. This effect was accompanied by a concomitant augment in the relative transcript abundance of HSPA1A and a decrease of BAX. Furthermore, the addition of GSH to vitrification-warming media increased survival rates at post-warming. Likewise, the action of GSH was concomitant with an increase in the relative abundance of GPX1 and a decrease of BAX transcript. Blastocyst rates of vitrified-warmed oocytes did not differ from their fresh counterparts when IGF-I and GSH were combined. In conclusion, supplementing maturation medium with 100 ng·mL−1 IGF-I and vitrification-warming solutions with 2 mM GSH improves the quality and cryotolerance of IVM pig oocytes, through a mechanism that involves BAX, GPX1 and HSPA1A expression.

show abstract

Thermoprotective effect of insulin-like growth factor 1 on in vitro matured bovine oocyte exposed to heat shock

Cited by 28 publications

References 72 publications

Autophagy is a pro-survival adaptive response to heat shock in bovine cumulus-oocyte complexes

Autophagy is a pro-survival adaptive response to heat shock in bovine cumulus-oocyte complexes

Endoplasmic reticulum distribution during bovine oocyte activation is regulated by protein kinase C via actin filaments

Supplementing Maturation Medium With Insulin Growth Factor I and Vitrification-Warming Solutions With Reduced Glutathione Enhances Survival Rates and Development Ability of in vitro Matured Vitrified-Warmed Pig Oocytes

Contact Info

Product

Resources

About