“…45−47 In contrast, Cr(III) can be naturally reoxidized by manganese oxides (MnO x ) and hydrogen peroxide (H 2 O 2 ) in aerobic and anaerobic soils, respectively. 48,49 In this study, the soil was rich in organic matter (1.78 ± 0.19%) and Fe (46,600 ± 6614 mg/ kg) but contained a low level of Mn (126 ± 8 mg/kg), hinting at the potential of natural chemical Cr(VI) reduction and explaining a Cr(VI) reduction efficiency of 12.6% in NC treatment (sterile soil; Figure 2).…”
Section: Mechanisms Of Chemical and Microbial Cr(vi)mentioning
confidence: 70%
“…Cr(VI) chemical reduction is mainly driven by Fe-rich minerals and soil organic matter, e.g., chlorite, pyrite, goethite (α-FeO(OH)), hematite (α-Fe 2 O 3 ), and humus. − In particular, structural Fe(II) in minerals has a stronger reduction capacity and is the main redox-active component . For instance, structural Fe(II) in magnetite (Fe 3 O 4 ) is reported to reduce Cr(VI) and form inner-sphere complexes. , Soil humus has many functional groups, e.g., phenolic hydroxyl, with extra electrons to reduce Cr(VI). − In contrast, Cr(III) can be naturally reoxidized by manganese oxides (MnO x ) and hydrogen peroxide (H 2 O 2 ) in aerobic and anaerobic soils, respectively. , In this study, the soil was rich in organic matter (1.78 ± 0.19%) and Fe (46,600 ± 6614 mg/kg) but contained a low level of Mn (126 ± 8 mg/kg), hinting at the potential of natural chemical Cr(VI) reduction and explaining a Cr(VI) reduction efficiency of 12.6% in NC treatment (sterile soil; Figure ).…”
Chromium (Cr) is a heavy metal with a high toxicity and pathogenicity. Microbial reduction is an effective strategy to remove Cr(VI) at contaminated sites but suffers from the low populations and activities of Cr-reducing microorganisms in soils. This study proposed an in situ sonoporation-mediated gene transfer approach, which improved soil Cr(VI) reduction performance by delivering exogenous Cr-transporter chrA genes and Cr-reducing yieF genes into soil microorganisms with the aid of ultrasound. Besides the increasing populations of Cr-resistant bacteria and elevated copy numbers of chrA and yieF genes after sonoporation-mediated gene transfer, three new Cr-reducing strains were isolated, among which Comamonas aquatica was confirmed to obtain Cr-resistant capability. In addition, sonoporation-mediated gene transfer was the main driving force significantly shaping soil microbial communities owing to the predominance of Cr-resistant microbes. This study pioneered and evidenced that in situ soil sonoporation-mediated gene transfer could effectively deliver functional genes into soil indigenous microbes to facilitate microbial functions for enhanced bioremediation, e.g., Cr-reduction in this study, showing its feasibility as a chemically green and sustainable remediation strategy for heavy metal contaminated sites.
“…45−47 In contrast, Cr(III) can be naturally reoxidized by manganese oxides (MnO x ) and hydrogen peroxide (H 2 O 2 ) in aerobic and anaerobic soils, respectively. 48,49 In this study, the soil was rich in organic matter (1.78 ± 0.19%) and Fe (46,600 ± 6614 mg/ kg) but contained a low level of Mn (126 ± 8 mg/kg), hinting at the potential of natural chemical Cr(VI) reduction and explaining a Cr(VI) reduction efficiency of 12.6% in NC treatment (sterile soil; Figure 2).…”
Section: Mechanisms Of Chemical and Microbial Cr(vi)mentioning
confidence: 70%
“…Cr(VI) chemical reduction is mainly driven by Fe-rich minerals and soil organic matter, e.g., chlorite, pyrite, goethite (α-FeO(OH)), hematite (α-Fe 2 O 3 ), and humus. − In particular, structural Fe(II) in minerals has a stronger reduction capacity and is the main redox-active component . For instance, structural Fe(II) in magnetite (Fe 3 O 4 ) is reported to reduce Cr(VI) and form inner-sphere complexes. , Soil humus has many functional groups, e.g., phenolic hydroxyl, with extra electrons to reduce Cr(VI). − In contrast, Cr(III) can be naturally reoxidized by manganese oxides (MnO x ) and hydrogen peroxide (H 2 O 2 ) in aerobic and anaerobic soils, respectively. , In this study, the soil was rich in organic matter (1.78 ± 0.19%) and Fe (46,600 ± 6614 mg/kg) but contained a low level of Mn (126 ± 8 mg/kg), hinting at the potential of natural chemical Cr(VI) reduction and explaining a Cr(VI) reduction efficiency of 12.6% in NC treatment (sterile soil; Figure ).…”
Chromium (Cr) is a heavy metal with a high toxicity and pathogenicity. Microbial reduction is an effective strategy to remove Cr(VI) at contaminated sites but suffers from the low populations and activities of Cr-reducing microorganisms in soils. This study proposed an in situ sonoporation-mediated gene transfer approach, which improved soil Cr(VI) reduction performance by delivering exogenous Cr-transporter chrA genes and Cr-reducing yieF genes into soil microorganisms with the aid of ultrasound. Besides the increasing populations of Cr-resistant bacteria and elevated copy numbers of chrA and yieF genes after sonoporation-mediated gene transfer, three new Cr-reducing strains were isolated, among which Comamonas aquatica was confirmed to obtain Cr-resistant capability. In addition, sonoporation-mediated gene transfer was the main driving force significantly shaping soil microbial communities owing to the predominance of Cr-resistant microbes. This study pioneered and evidenced that in situ soil sonoporation-mediated gene transfer could effectively deliver functional genes into soil indigenous microbes to facilitate microbial functions for enhanced bioremediation, e.g., Cr-reduction in this study, showing its feasibility as a chemically green and sustainable remediation strategy for heavy metal contaminated sites.
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