Thermophoretic glycan profiling of extracellular vesicles for triple-negative breast cancer management

Li, Yike; Zhang, Shaohua; Liu, Chao; Deng, Jinqi; Tian, Fei; Feng, Qiang; Qin, Lili; Bai, Lixiao; Fu, Ting; Zhang, Liqin; Wang, Yuguang; Sun, Jiashu

doi:10.1038/s41467-024-46557-5

Cited by 3 publications

(1 citation statement)

References 61 publications

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“…Lectins, known as carbohydrate-binding proteins, have been extensively utilized to elucidate the global glycome of sEV preparations. − Mass spectrometry represents another versatile platform for profiling complex glycan structures and even identifying glycosylation sites of proteins within sEV isolates. , Recently, novel approaches have emerged, including DNA assemblies combined with lectin recognition, metabolic glycan labeling, and chemoenzymatic-based strategy, for exploring protein-specific glycosylation on sEVs. − Nonetheless, the glycan analysis strategies mentioned above primarily conduct ensemble-averaged analysis of sEV glycans. It is worth noting that sEVs exhibit high heterogeneity in both protein and nucleic acid compositions, as evidenced by single-sEV technologies such as fluorescence-based microscopy imaging, droplet digital PCR (ddPCR), and nano-flow cytometry (nFCM). − Whether sEVs are uniformly glycosylated or display heterogeneous glycosignatures remains to be investigated …”

Section: Introductionmentioning

confidence: 99%

Surface Sialic Acid Detection of Small Extracellular Vesicles at the Single-Particle Level by Nano-Flow Cytometry

Cai,

Zhan,

Chen

et al. 2024

Anal. Chem.

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Glycans, particularly sialic acids (SAs), play crucial roles in diverse biological processes. Despite their significance, analyzing specific glycans, such as sialic acids, on individual small extracellular vesicles (sEVs) has remained challenging due to the limited glycan capacity and substantial heterogeneity of sEVs. To tackle this issue, we introduce a chemical modification method of surface SAs on sEVs named PALEV-nFCM, which involves periodate oxidation and aniline-catalyzed oxime ligation (PAL), in conjunction with single-particle analysis using a laboratory-built nano-flow cytometer (nFCM). The specificity of the PALEV labeling method was validated using SA-decorated liposomes, enzymatic removal of terminal SA residues, lectin preblocking, and cellular treatment with an endogenous sialyltransferase inhibitor. Comprehensive mapping of SA distributions was conducted for sEVs derived from different sources, including conditioned cell culture medium (CCCM) of various cell lines, human saliva, and human red blood cells (RBCs). Notably, treatment with the calcium ionophore substantially increases the population of SA-positive RBC sEVs and enhances the SA content on individual RBC sEVs as well. nFCM provides a sensitive and versatile platform for mapping SAs of individual sEVs, which could significantly contribute to resolving the heterogeneity of sEVs and advancing the understanding of their glycosignature.

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Section: Introductionmentioning

confidence: 99%

Surface Sialic Acid Detection of Small Extracellular Vesicles at the Single-Particle Level by Nano-Flow Cytometry

Cai,

Zhan,

Chen

et al. 2024

Anal. Chem.

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Integrated photothermal microcarriers for precise exosome‐secreted microRNA profiling in breast cancer diagnosis

Shi,

Cheng,

Chen

et al. 2024

Quant. Biol.

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Breast cancer constitutes a significant global health burden, while conventional diagnosis approaches may lack precision and can be discomforting for patients. Exosomes have emerged as promising biomarkers for breast cancer due to their participation in diverse pathological processes, and a convenient analysis platform is believed to greatly promote its application. In this study, we propose a novel digital PCR approach utilizing near‐infrared (NIR) photo‐responsive thermosensitive microcarriers integrated with black phosphorus for quantifying microRNA (miRNA) biomarkers within exosomes. Petal‐like biomimetic nanomaterials were firstly assembled for non‐specific exosome capture based on the affinity effect of avidin and biotin. Photothermal‐responsive microcarriers, fabricated using gelatin‐based substrates blended with photothermal nanocomposite, exhibited NIR‐induced heating and reversible phase transition properties. We optimized synthesis parameters on thermal response and established a programmable and controllable NIR light source module. The results indicated a significant elevation in the levels of biomarkers miRNA‐1246 and miRNA‐122, with fold increases ranging from 6.2 to 23.6 and 5.9 to 13.0, respectively, in breast cancer cell lines MCF‐7 and MDA‐MB‐231 compared to healthy control cells HUVEC. This study offers broad prospects for utilizing exosomes to resolve predictive biomarkers.

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Colloid thermophoresis in surfactant solutions: Probing colloid–solvent interactions through microscale experiments

Pu,

Panahi,

Natale

et al. 2024

The Journal of Chemical Physics

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Thermophoresis has emerged as a powerful tool for characterizing and manipulating colloids at the nano- and micro-scales due to its sensitivity to colloid–solvent interactions. The use of surfactants enables the tailoring of surface chemistry on colloidal particles and the tuning of interfacial interactions. However, the microscopic mechanisms underlying thermophoresis in surfactant solutions remain poorly understood due to the complexity of multiscale interaction coupling. To achieve a more fundamental understanding of the roles of surfactants, we investigated the thermophoretic behavior of silica beads in both ionic and nonionic surfactant solutions at various background temperatures. We provide a complete mechanistic picture of the effects of surfactants on interfacial interactions through mode-coupling analysis of both electrophoretic and thermophoretic experiments. Our results demonstrate that silica thermophoresis is predominantly governed by the dissociation of silanol functional groups at silica–water interfaces in nonionic surfactant solutions, while in ionic surfactant solutions, the primary mechanism driving silica thermophoresis is the adsorption of ionic surfactants onto the silica surface.

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Thermophoretic glycan profiling of extracellular vesicles for triple-negative breast cancer management

Cited by 3 publications

References 61 publications

Surface Sialic Acid Detection of Small Extracellular Vesicles at the Single-Particle Level by Nano-Flow Cytometry

Surface Sialic Acid Detection of Small Extracellular Vesicles at the Single-Particle Level by Nano-Flow Cytometry

Integrated photothermal microcarriers for precise exosome‐secreted microRNA profiling in breast cancer diagnosis

Colloid thermophoresis in surfactant solutions: Probing colloid–solvent interactions through microscale experiments

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