Thermomyces lanuginosus STm: A source of thermostable hydrolytic enzymes for novel application in extraction of high-quality natural rubber from Taraxacum kok-saghyz (Rubber dandelion)

Abstract

Hydrolytic enzymes from a newly isolated strain of the thermophilic fungus Thermomyces lanuginosus were used to extract rubber from Taraxacum kok-saghyz commonly known as rubber (or Russian or Kazak(h)) dandelion. The fungus was isolated from garden soil and identified as Thermomyces lanuginosus STm based on 18S rRNA gene sequence analysis. The isolate produced considerable amounts of extracellular hydrolytic enzymes on lignocellulosic substrates at 55°C incubated for 8 days in 150 mL shake flask experiments. The maximum enzyme activities on wheat straw and guayule bagasse were: xylanase (167.41; 130.1 U/mg), inulinase (69.8; 34.1 U/mg), cellulase (carboxymethyl cellulase) (16.7; 4.8 U/mg), filter paper assay (FPase) (14.2; 5.5 FPU g-1) and pectinase (7.2; 3.2 U/mg), respectively. In addition, alkalipretreated roots of Taraxacum kok-saghyz (TK), incubated with crude enzyme extracts from T. lanuginosus STm grown on guayule bagasse, subsequently yielded more natural rubber (90 mg/g dry TK root) than previously established protocols, Eskew process (24 mg/g) and commercialenzyme-combination process (45 mg/g). Rubber purity in the T. lanuginosus STm treatment was 71.7%, greater than the Eskew process at 37.5%. However, the crude T. lanuginosus STm enzyme treatment at 91.6% rubber purity approached the purity of the commercial-enzymecombination process 94.1% purity.

Avaliação Microbiológica De Uma Cerveja Tipo Pilsen Com Adição De Chá Verde Na Etapa De Maturação

“…Silva et al (2019) reportaram a atividade antibacteriana de uma lectina da sarcotesta da fruta Punica granatum (PgTeL) contra cinco isolados de E. coli resistentes a drogas capazes de produzir β-lactamases, com valores de CMI e CMB variando de 12,5 a 50,0 μg / mL e de 25,0 a 100,0 μg / mL, respectivamente.…”

Research progress on sustainability of key tire materials

“…DR is mainly found in the latex tubes in the roots of Taraxacum kok-saghyz (TKS), with rubber contents ranging from 5 to 24 wt.%. This rubber can be extracted using methods, such as solvent, dry grinding, wet grinding, enzyme digestion, or acid-base neutralization, to obtain solid DR. [17][18][19] Because of the wider distribution and its advantages, which include a short growth cycle, high yield, unrestricted planting conditions, and climate requirements, and the absence of allergenic proteins in the extracted rubber, DR has the potential to replace NR in tire manufacturing. DR has a cis-polyisoprene structure, and its relative molecular weight is similar to that of NR.…”

Rapid Rubber Extraction and NMR Spectroscopy of Rubber, Extracted from the Endemic Species Scorzonera Tau-Saghyz

“…For example, alkaline pretreatment of biopolymer results in partial solubilization of organic carbon and proteins, reduc-Eurasian Chemico-Technological Journal 22 (2020) 59-68 ing fiber content and thereby releasing rubber from root tissues [30]. However, in this case, the yield of rubber is lower than the extraction of natural rubber from Taraxacum kok-saghyz by means of commercial hydrolytic enzymes (45 mg/g from dry roots) [31].…”