Thermoluminescence as a tool for monitoring ozone-stressed plants

Skotnica, Jiří; Gilbert, Matthias; Weingart, Ilka; Wilhelm, Christian

doi:10.1016/s0269-7491(02)00365-2

Cited by 7 publications

(7 citation statements)

References 13 publications

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“…The effects of ozone exposure, aging of irradiated leaves, and transfer of plants from dark to light on TL glow curves: Following ozone exposure, the peak-temperature of the band being at 22 °C in control (based on its properties denoted as TL B-band, see Skotnica et al 2003) was shifted to lower temperature and a TL band appeared at 55 °C (Fig. 1A).…”

Section: Resultsmentioning

confidence: 99%

“…In a previous report (Skotnica et al 2003) it was demonstrated that ozone induces changes in TL glow curves of barley leaves measured after excitation by 1 single turnover flash. The characteristic effects of ozone on TL were a shift of the peak temperature of the B band (maximum at 22 °C in control) to lower temperatures, an increase of the band at about 55 °C together with a stimulated light emission in the temperature range from 60 to 160 °C.…”

Section: Introductionmentioning

confidence: 94%

“…In the case of infiltration of leaves or the aging, a 2 cm long central part of the 3 cm segment was used as the sample for measurements. TL was measured using a set-up described in Skotnica et al (2003) or similar to Ducruet et al (1998). The sample was placed on the sample holder, a Peltier plate 4×4 cm (HT6-12-40, Melcor, USA) covered by a thin copper film.…”

Section: Fluorescence and Thermoluminescence Measurementsmentioning

confidence: 99%

“…The ozone exposure of leaves induced a downshift of the peak temperature of the B band. Generally, it can be caused by a modification of donor as well as acceptor side of PS2 (see Vass and Inoue 1992). In literature, the down-shift of the B-band was mainly explained by the increase of the trans-thylakoid pH gradient or by the increased reduction state of the plastoquinone pool (for example see Miranda and Ducruet 1995).…”

Section: The B-bandmentioning

confidence: 99%

“…Various TL bands peaking at different temperatures have been described. The majority of TL bands originate from a thermally stimulated recombination of positive charges on the donor side of photosystem 2 (PS2) with electrons on the acceptor side (for review see Vass and Inoue 1992). Changes in the intensities and positions of these TL bands yield valuable information about the functional state of electron transport in PS2.…”

Section: Introductionmentioning

confidence: 99%

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The mechanism of the ozone-induced changes in thermoluminescence glow curves of barley leaves

Skotnica¹,

Gilbert²,

Weingart³

et al. 2005

Photosynt.

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The changes in thermoluminescence (TL) signals induced by short-term ozone exposure of leaves are characterized by a down-shift of the peak-temperature of the TL B-band and an increase of a TL band at 55 °C. We investigated the relationship of these changes to photosystem 2 (PS2) photochemistry. The changes were not only detectable in the presence of ozone, but also after irradiation of dark-adapted leaves and after aging of irradiated detached leaf segments. The opposite effect on TL, an up-shift of the peak-temperature of the B-band and the decrease of the intensity of the band at 55 °C were found after infiltration of leaves with nigericin, antimycin A, and diphenyleneiodonium chloride (DPI). Propyl gallate down-shifted the peak-temperature of the B-band. 2,5-dimethyl-1,4-benzoquinone up-shifted the peaktemperature of the B-band and decreased the intensity of the 55 °C band. The intensity of the 55 °C band did not change significantly in the presence of oxygen in comparison to that in nitrogen atmosphere. It decreased with time of dark adaptation (50 % intensity was observed after 3 h of dark adaptation at room temperature), however, it was reactivated to its initial value (at 5 min of dark adaptation) after 1 single-turnover flash. The 55 °C band was not significantly changed in the presence of DCMU. Thus the ozone-induced band at 55 °C is assigned to charge recombination in PS2. Changes in the electron transport chain at the acceptor side of PS2, probably related to the cyclic electron transport around photosystem 1 and/or chlororespiration, could play an important role in the increase of the 55 °C band and the down-shift of the B-band. The changes at the acceptor side indicated by TL can be an expression of a physiological regulatory mechanism functional under stress conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Section: Fluorescence and Thermoluminescence Measurementsmentioning

confidence: 99%

Section: The B-bandmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The mechanism of the ozone-induced changes in thermoluminescence glow curves of barley leaves

Skotnica¹,

Gilbert²,

Weingart³

et al. 2005

Photosynt.

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show abstract

Thermoluminescence

et al. 2011

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Effect of chromate on photosynthesis in Cr(VI)-resistant Chlorella

Yewalkar¹,

Dhumal²,

Sainis³

2013

Photosynt.

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Chromate-resistant Chlorella spp. isolated from effluents of electroplating industry could grow in the presence of 30 µM K 2 Cr 2 O 7 . Since photosynthesis is sensitive to oxidative stress, chromate toxicity to photosynthesis was examined in this algal isolate. Chromate [Cr(VI)] up to 100 µM was found to stimulate photosynthesis, while 90% inhibition was found, when the cells were incubated with 1 mM Cr(VI) for 4 h. Photosystem (PS) II was inhibited by 80% and PSI by 40% after such Cr(VI) treatment. Thermoluminescence studies on cells treated with 1 mM Cr(VI) for 4 h showed that S 2 Q A recombination peak (Q) was shifted to higher temperature, whereas S 2 /S 3 Q B recombination peak (B) was shifted to lower temperature. These shifts indicated alga stress response in order to overcome an excitation stress resulting from the inhibition of photosynthesis by Cr(VI). The nontreated Chlorella cells kept in the dark showed periodicity of four for the Q peak (4-8ºC) and B peak (34-38°C) after exposure to series of single, turnover, saturating flashes. This periodicity was lost in Cr(VI)-treated cells. Higher concentrations of Cr(VI) inhibited mainly the electron flow in the electron transport chain, inactivated oxygen evolving complex, and affected also Calvin cycle enzymes in the Cr(VI)-resistant isolates of Chlorella.

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Thermoluminescence as a tool for monitoring ozone-stressed plants

Cited by 7 publications

References 13 publications

The mechanism of the ozone-induced changes in thermoluminescence glow curves of barley leaves

The mechanism of the ozone-induced changes in thermoluminescence glow curves of barley leaves

Thermoluminescence

Effect of chromate on photosynthesis in Cr(VI)-resistant Chlorella

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