Thermoforming techniques for manufacturing porous scaffolds for application in 3D cell cultivation

Borowiec, Justyna; Hampl, Jörg; Gebinoga, Michael; Elsarnagawy, Tarek; Elnakady, Yasser A.; Fouad, Hassan; Almajhadi, Fahd; Fernekorn, Uta; Weise, Frank; Singh, Sukhdeep; Elsarnagawy, Dief; Schober, Andreas

doi:10.1016/j.msec.2015.01.002

Cited by 10 publications

(12 citation statements)

References 25 publications

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“…For example, PLA-based silver-ions-including nanofibers with anti-microbial properties were tested successfully in vitro for the development of skin wound dressings [ 30 ]. In a recent study by Borowiec et al [ 31 ], a 3D scaffold was created from PLA, which demonstrated better biocompatibility and higher albumin secretion (a mature, differentiated hepatocyte function) of the hepatic cell line HepG2 compared to those obtained with PC scaffolds. We observed higher cell numbers and lower metabolic activity in cultures of BM-MSCs on PC than those for PLA, indicative of PLA inducing more differentiation.…”

Section: Discussionmentioning

confidence: 99%

Response of Primary Human Bone Marrow Mesenchymal Stromal Cells and Dermal Keratinocytes to Thermal Printer Materials In Vitro

et al. 2016

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Advancement in thermal three-dimensional printing techniques has greatly increased the possible applications of various materials in medical applications and tissue engineering. Yet, potential toxic effects on primary human cells have been rarely investigated. Therefore, we compared four materials commonly used in thermal printing for bioengineering, namely thermally printed acrylonitrile butadiene styrene, MED610, polycarbonate, and polylactic acid, and investigated their effects on primary human adult skin epidermal keratinocytes and bone marrow mesenchymal stromal cells (BM-MSCs) in vitro. We investigated indirect effects on both cell types caused by potential liberation of soluble substances from the materials, and also analyzed BM-MSCs in direct contact with the materials. We found that even in culture without direct contact with the materials, the culture with MED610 (and to a lesser extent acrylonitrile butadiene styrene) significantly affected keratinocytes, reducing cell numbers and proliferation marker Ki67 expression, and increasing glucose consumption, lactate secretion, and expression of differentiation-associated genes. BM-MSCs had decreased metabolic activity, and exhibited increased cell death in direct culture on the materials. MED610 and acrylonitrile butadiene styrene induced the strongest expression of genes associated to differentiation and estrogen receptor activation. In conclusion, we found strong cell-type-specific effects of the materials, suggesting that materials for applications in regenerative medicine should be carefully selected not only based on their mechanical properties but also based on their cell-type-specific biological effects.Electronic supplementary materialThe online version of this article (doi:10.1007/s40846-016-0118-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Response of Primary Human Bone Marrow Mesenchymal Stromal Cells and Dermal Keratinocytes to Thermal Printer Materials In Vitro

et al. 2016

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“…Many techniques are utilized to fabricate the scaffolds in tissue engineering. The solvent-free techniques, for example, injection [19], extrusion [20], thermoforming [21], selective laser sintering [22][23][24], and fused deposition modeling (FDM) [7,9,25,26], have drawn attention in the field of scaffolding. FDM can produce three-dimensional objects directly from the filaments of melted polymer or resin assisted by the computer-aided design program.…”

Section: Introductionmentioning

confidence: 99%

Poly(ε-caprolactone)/Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Blend from Fused Deposition Modeling as Potential Cartilage Scaffolds

Kosorn

Wutticharoenmongkol

2021

International Journal of Polymer Science

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The scaffolds of poly(ε-caprolactone)/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PCL/PHBV) blends were fabricated from fused deposition modeling. From indirect cytotoxicity testing based on mouse fibroblasts, all scaffolds with various blend ratios were nontoxic to cells. The surface-treated scaffold with a blend ratio of 25/75 PCL/PHBV exhibited the highest proliferation of porcine chondrocytes and total glycosaminoglycans (GAGs) after 21 days of culture. The scaffolds with a blend ratio of 25/75 with local pores (LP) were prepared from FDM along with a salt leaching technique using NaCl as porogens. The effect of NaOH in surface treatment on the biological property of scaffolds was investigated. The scaffolds with LP and with 1 M NaOH surface treatment exhibited the highest proliferation of cells and total GAGs after 28 days of culture. The degradation behaviors of the scaffolds were studied. The nonsurface treated, surface treated without LP, and surface treated with LP scaffolds were degraded in phosphate buffer (pH 7.4) for 30 days at 37°C and 50°C for nonenzymatic condition and at 37°C for enzymatic condition. The surface treated with LP scaffold showed the highest amount of weight loss, followed by the surface treated without LP, and the nonsurface-treated scaffolds without LP, respectively. The results from Fourier-transform infrared spectroscopy indicated degradation of PCL and PHBV through hydrolysis of the ester functional group. The compressive strengths of all scaffolds were sufficiently high. The results suggested that the scaffolds with the existence of LP and with surface treatment showed the highest potential for use as cartilage scaffolds.

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“…Among various biocompatible plastics, PC appears to be a suitable material of choice for biotechnological applications due to its (i) availability as porous membranes with controllable pore size [ 13 ], (ii) micro thermoformability that is useful for generating three-dimensional microgeometries and vessel-like structures [ 14 ] and (iii) facile surface chemistry [ 15 ]. Recently, we have reported a photoswitching behaviour of photosensitive DASA on the polymer surface.…”

Section: Resultsmentioning

confidence: 99%

Donor–acceptor Stenhouse adduct-grafted polycarbonate surfaces: selectivity of the reaction for secondary amine on surface

et al. 2018

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Donor–acceptor Stenhouse adducts (DASAs) are gaining attention from organic and material chemists due to their visible light-stimulated photochromic properties. In this report, we present a facile method for grafting coloured triene on polycarbonate surface, without involving any pre-treatments like plasma activation, etc. The chemoselectivity of carbonate with a primary amine and Meldrum's activated furan (MAF) with polymer bound secondary amine has been exploited to graft photoswitchable DASA on the polymer surface. Primary, secondary and tertiary amine-functionalized polycarbonate surfaces have been prepared to evaluate the reactivity of amine with MAF.

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Thermoforming techniques for manufacturing porous scaffolds for application in 3D cell cultivation

Cited by 10 publications

References 25 publications

Response of Primary Human Bone Marrow Mesenchymal Stromal Cells and Dermal Keratinocytes to Thermal Printer Materials In Vitro

Response of Primary Human Bone Marrow Mesenchymal Stromal Cells and Dermal Keratinocytes to Thermal Printer Materials In Vitro

Poly(ε-caprolactone)/Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Blend from Fused Deposition Modeling as Potential Cartilage Scaffolds

Donor–acceptor Stenhouse adduct-grafted polycarbonate surfaces: selectivity of the reaction for secondary amine on surface

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