Thermodynamics of multilayer protein adsorption on a gold nanoparticle surface

Das, Puspendu K.; Mishra, Akriti

doi:10.1039/d2cp02439j

Cited by 4 publications

(1 citation statement)

References 69 publications

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“…An important difference between these products is whether the antibodies form only one layer or multiple layers on the surface of the particles. Complexes with polylayer immobilization of antibodies on the GNP surfaces have been considered in several studies [18][19][20]. The binding affinity upon the formation of the outer layers is much is lower than the affinity for the first layer, which is why these layers are called soft and hard protein coronas, respectively [21][22][23].…”

Section: Preparation Of Antibody-gnp Conjugates and Determination Of ...mentioning

confidence: 99%

Ability of Antibodies Immobilized on Gold Nanoparticles to Bind Small Antigen Fluorescein

Sotnikov,

Byzova,

Zherdev

et al. 2023

IJMS

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The analytical applications of antibodies are often associated with their immobilization on different carriers, which is accompanied by a loss of antigen-binding activity for a sufficient proportion of the bound antibodies. In contrast to data on plain carriers, minimal data are available on the properties of antibodies on the surfaces of nanoparticles. Protein antigens have been predominantly investigated, for which space restrictions do not allow them to occupy all active sites of immobilized antibodies. This study considered a low-molecular-weight compound, fluorescein, as an antigen. Spherical gold nanoparticles with five different sizes, two differently charged forms of fluorescein, and three different levels of surface coverage by immobilized antibodies were tested. For gold nanoparticles with diameters from 14 to 35.5 nm with monolayers of immobilized antibodies, the percentage of molecules capable of binding carboxyfluorescein varied from 6% to 17%. The binding of aminofluorescein was more efficient; for gold nanoparticles with an average diameter of 21 nm, the percentage of active binding sites for the immobilized antibodies reached 27% compared with 13% for the carboxyfluorescein case. A fourfold reduction in the coverage of the nanoparticles’ surface compared with that of the monolayer did not lead to reliable changes in the percentage of active binding sites. The obtained data demonstrate that an antigen’s binding to immobilized antibodies is limited even for small antigens and depends on the size of the nanoparticles and the electrostatic repulsion near their surface.

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Section: Preparation Of Antibody-gnp Conjugates and Determination Of ...mentioning

confidence: 99%

Ability of Antibodies Immobilized on Gold Nanoparticles to Bind Small Antigen Fluorescein

Sotnikov,

Byzova,

Zherdev

et al. 2023

IJMS

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show abstract

Preparation of Langmuir-Blodgett composite films based on sericin protein and silk fibroin with good surface-enhanced Raman scattering and photovoltaic properties

Zhao,

Wang,

Wei

et al. 2024

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Monolayer protein adsorption on cationic gold nanoparticles: A thermodynamic study

Saikia

Das

2023

Chemical Physics Letters

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Thermodynamics of multilayer protein adsorption on a gold nanoparticle surface

Abstract: We report the thermodynamics of protein adsorption on negatively charged colloidal gold nanoparticles (GNPs) of 16 nm to 69 nm at pH 7.0. Three biologically important proteins of varying size,...

Cited by 4 publications

References 69 publications

Ability of Antibodies Immobilized on Gold Nanoparticles to Bind Small Antigen Fluorescein

Ability of Antibodies Immobilized on Gold Nanoparticles to Bind Small Antigen Fluorescein

Preparation of Langmuir-Blodgett composite films based on sericin protein and silk fibroin with good surface-enhanced Raman scattering and photovoltaic properties

Monolayer protein adsorption on cationic gold nanoparticles: A thermodynamic study

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