Thermodynamics of citrate complexation with Mn2+, Co2+, Ni2+ and Zn2+ ions

Wyrzykowski, Dariusz; Chmurzyński, Lech

doi:10.1007/s10973-009-0523-4

Cited by 84 publications

(51 citation statements)

References 26 publications

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“…2a and b) is not 255 known, but neutralization reactions and ligand binding of weak acids onto organic material are 256 known to cause substantial exothermic reactions 25, 34 . In non-sterile soil it is, however, uncertain 257 if abiotic and biotic reactions have similar strengths or if one of them is a stronger sink for 258 breakdown of carboxylic acids.…”

Section: Results and Discussion 243mentioning

confidence: 99%

Isothermal Microcalorimetry Provides New Insight into Terrestrial Carbon Cycling

Herrmann

Coucheney

Nunan

2014

Environ. Sci. Technol.

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Energy is continuously transformed in environmental systems through the metabolic activities of living organisms, but little is known about the relationship between the two. In this study, we tested the hypothesis that microbial energetics are controlled by microbial community composition in terrestrial ecosystems. We determined the functional diversity profiles of the soil biota (i.e., multiple substrate-induced respiration and microbial energetics) in soils from an arable ecosystem with contrasting long-term management regimes (54 years). These two functional profiling methods were then related to the soils’ microbial community composition. Using isothermal microcalorimetry, we show that direct measures of energetics provide a functional link between energy flows and the composition of below-ground microbial communities at a high taxonomic level (Mantel R = 0.4602, P = 0.006). In contrast, this link was not apparent when carbon dioxide (CO2) was used as an aggregate measure of microbial metabolism (Mantel R = 0.2291, P = 0.11). Our work advocates that the microbial energetics approach provides complementary information to soil respiration for investigating the involvement of microbial communities in below-ground carbon dynamics. Empirical data of our proposed microbial energetics approach can feed into carbon-climate based ecosystem feedback modeling with the suggested conceptual ecological model as a base.

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Section: Results and Discussion 243mentioning

confidence: 99%

Isothermal Microcalorimetry Provides New Insight into Terrestrial Carbon Cycling

Herrmann

Coucheney

Nunan

2014

Environ. Sci. Technol.

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“…It is connected with the transfer of the proton from the ligand to the buffer. Carrying out the ITC measurements in buffer solutions of equal pH but different enthalpies of proton association of its components (Mes, Caco, Pipes), DH BH , made it possible to determine the enthalpy of complex formation, independent of the nature of the buffer used [16][17][18]. Moreover, when the enthalpy of the metal-ligand (strong ligand) reaction is known, DH MNTA o , the metalbuffer (weak ligand) enthalpy can be calculated, DH MB o .…”

Section: Resultsmentioning

confidence: 99%

Investigation of metal–buffer interactions using isothermal titration calorimetry

Wyrzykowski

Pilarski²,

Jacewicz

et al. 2012

J Therm Anal Calorim

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Isothermal titration calorimetry (ITC) and potentiometric titration (PT) methods were used to study the interactions of cobalt(II) and nickel(II) ions with buffer substances 2-(N-morpholino)ethanesulfonic acid (Mes), dimethylarsenic acid (Caco), and piperazine-N,N 0 -bis (2-ethanesulfonic acid) (Pipes). Based on the results of PT data, the stability constants were calculated for the metal-buffer complexes (T = 298.15 K, ionic strength I = 100 mM NaClO 4 ). Furthermore, calorimetric measurements (ITC) were run in 100 mM Mes, Caco, and Pipes solutions with pH 6, at 298.15 K. The enthalpies (DH) of the metalbuffer complexation reactions were calculated indirectly by displacement titration using nitrilotriacetic acid (H 3 NTA) as a strong-binding, competitive ligand. Finally, to verify obtained results, the number of protons released by H 3 NTA due to complexation of the cobalt(II) and nickel(II) ions was determined from calorimetric data and compared with results of calculations.

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“…The reference cell contained the distilled water. All details of the measuring devices and the experimental set-up were described previously [17][18][19]. All reagents were dissolved directly into 50 mM buffer solution of HEPES or Tricine of pH 8.0, containing 20 mM CaCl 2 .…”

Section: Isothermal Titration Calorimetry (Itc)mentioning

confidence: 99%

Interactions between trypsin and its peptidic inhibitors studied by isothermal titration calorimetry (ITC)

Dębowski

Wyrzykowski

Lubos

et al. 2015

J Therm Anal Calorim

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Isothermal titration calorimetry (ITC) technique was used to study the interactions of trypsin with bicyclic sunflower-derived trypsin inhibitor (SFTI-1) as well as with its new monocyclic (with disulphide bridge only) analogues (C 3 H 5 O)-SFTI-1 and (C 8 H 15 O)-SFTI-1. ITC measurements were run in 50 mM buffer solution of HEPES or Tricine of pH 8, containing 20 mM CaCl 2 at 298.15 K. Based on calorimetric data, the equilibrium constants for the inhibitor-enzyme-binding processes, K, the binding stoichiometry, N (inhibitor-to-enzyme molar ratio), as well as thermodynamic parameters (DG, DH, DS) for the reactions were determined. The study revealed that the stoichiometry of the resulting complexes equals 1:1. The negative binding enthalpy (D ITC H) and favourable entropy factor (TD ITC S) suggest an important contribution of hydrogen bonding as well as hydrophobic interactions to the inhibitor-enzyme affinity. Furthermore, the relationship between the modification of the peptide structure, the experimental conditions and the thermodynamic parameters has been discussed.

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Thermodynamics of citrate complexation with Mn2+, Co2+, Ni2+ and Zn2+ ions

Cited by 84 publications

References 26 publications

Isothermal Microcalorimetry Provides New Insight into Terrestrial Carbon Cycling

Isothermal Microcalorimetry Provides New Insight into Terrestrial Carbon Cycling

Investigation of metal–buffer interactions using isothermal titration calorimetry

Interactions between trypsin and its peptidic inhibitors studied by isothermal titration calorimetry (ITC)

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