Thermodynamics and structure of model bio-membrane of liver lipids in presence of imidazolium-based ionic liquids

Mitra, Saheli; Sharma, V. K.; Mitra, Jyotsna Bhatt; Chowdhury, Subhadip; Mukhopadhyay, Mrinmay K.; Mukhopadhyay, R.; Ghosh, Sajal K.

doi:10.1016/j.bbamem.2021.183589

Cited by 13 publications

(19 citation statements)

References 85 publications

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“…The surface pressure–area isotherm generated by a Langmuir trough is useful for studying interactions among amphiphilic molecules that form a single molecular layer at the air–water interface. ,,− Researchers have adopted this approach to understand the thermodynamics of the interaction of ILs with lipids. ,, It has been reported that the polar head group and hydrophobicity of lipids and the nature of anions and cations of ILs along with the pH of the environment play significant roles in this interaction. , Apart from electrostatics and van der Waals interactions, the thermodynamic state of a lipid in the membrane is important here . Further, as mentioned in the Introduction section, the interaction between a lipid and an IL may highly be influenced by the presence of other membranous component, such as cholesterol. , In the present study, the respective isotherms are generated by plotting the surface pressure against the area per molecule, which is calculated by considering the number of molecules of lipid (single component), lipid and IL (two components), and lipid, cholesterol, and IL (three components).…”

Section: Resultsmentioning

confidence: 99%

Cholesterol-Controlled Interaction of Ionic Liquids with Model Cellular Membranes

et al. 2023

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While ionic liquids (ILs) are considered as prospective ingredients of new antimicrobial agents, it is important to understand the adverse effects of these molecules on human cells. Since cholesterol is the essential component of a human cell membrane, in the present study, the effect of an imidazolium-based IL has been investigated on the model membrane in the presence of cholesterol. The area per sphingomyelin lipid is found to reduce in the presence of the IL, which is quantified by the area−surface pressure isotherm of the lipid monolayer formed at the air−water interface. The effect is considerably diminished in the cholesterolcontaining monolayer. Further, the IL is observed to decrease the rigidity of the cholesterol-free monolayer. Interestingly, the presence of cholesterol does not allow any change in this property of the layer at lower surface pressure. However, at a higher surface pressure, the IL increases the elasticity in the cholesterol-induced condensed phase of the lipid layer. The X-ray reflectivity measurement on a stack of cholesterol-free lipid bilayers proved the formation of IL-induced phase-separated domains in the matrix of a pure lipid phase. These domains are found to be formed by interdigitating the chains of the lipids, producing a thinner membrane. Such a phase is less intense in the cholesterol-containing membrane. All of these results indicate that the IL molecules may deform the cholesterol-free membrane of a bacterial cell, but the same may not be harmful to human beings as cholesterol could restrict the insertion in the cellular membrane of a human cell.

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Section: Resultsmentioning

confidence: 99%

Cholesterol-Controlled Interaction of Ionic Liquids with Model Cellular Membranes

et al. 2023

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“…DMIM-[Br] has the ability to penetrate PC membranes and align itself parallel to the lipid molecules. 62 The imidazolium ring forms interactions with the lipid head groups, while the alkyl chain extends deep into the hydrophobic region. Therefore, it is speculated that DMIM[Br] is also oriented parallel to the DHDAB membrane.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The effects of DMIM[Br] on the phase behavior of the DHDAB bilayer can be rationalized by understanding the complex interplay of hydrophobic interactions between the alkyl tails and electrostatic repulsion between the polar head groups, mediated by DMIM[Br], in the membrane. DMIM[Br] has the ability to penetrate PC membranes and align itself parallel to the lipid molecules . The imidazolium ring forms interactions with the lipid head groups, while the alkyl chain extends deep into the hydrophobic region.…”

Section: Resultsmentioning

confidence: 99%

Modulation of Phase Behavior and Microscopic Dynamics in Cationic Vesicles by 1-Decyl-3-methylimidazolium Bromide

Gupta,

Sharma,

Srinivasan

et al. 2023

Langmuir

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Synthetic cationic lipids have garnered significant attention as promising candidates for gene/DNA transfection in therapeutic applications. The phase behavior of the vesicles formed by these lipids is intriguing, revealing intricate connections to the structure and dynamics of the membrane. These phenomena emerge from the complex interplay between hydrophobic and electrostatic interactions of the lipids. In this study, we explore the impact of an ionic liquid-based surfactant, 1-decyl-3-methylimidazolium bromide (DMIM-[Br]), on the structural, dynamical, and phase behavior of cationic dihexadecyldimethylammonium bromide (DHDAB) vesicles. Our investigations indicate that the addition of DMIM[Br] increases the vesicle size while thinning the membrane. Further, DMIM[Br] also induces substantial changes in the membrane phase behavior. At 10 and 25 mol %, DMIM[Br] eliminates the pre-transition from coagel to intermediate crystalline (IC) phase and decreases the onset temperature of the main phase transition to the fluid phase. In the cooling cycle, the addition of DMIM[Br] further induces the formation of an intermediate gel phase. This behavior is reminiscent of the non-synchronous ordering observed in the DODAB membrane, a longer-chain counterpart of DHDAB. Interestingly, at 40 mol % of DMIM[Br], the formation of the intermediate gel phase is largely suppressed. Neutron scattering data provide evidence that the addition of DMIM[Br] enhances lipid mobility in coagel and fluid phases, suggesting that DMIM[Br] acts as a plasticizer, enhancing membrane fluidity across all of the phases. Our findings infer that DMIM[Br] modulates the membrane's phase behavior and fluidity, two essential ingredients for the efficient transport of cargo, by controlling the balance of electrostatic and hydrophobic interactions.

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“…344 The strength of the interaction of C n MIm BF 4 ILs (n = 2, 8, 10) with unilamellar vesicles (ULVs) from liver lipids depended on the alkyl side chain length. 345 C 10 MIm BF 4 caused disorder of the lipid monolayer 345 and shrinkage of multilamellar DPPC vesicles (MLV); 346 it also increased their surface charge and had a disordering effect on lipid chains in MLVs at concentrations below the CMC. 346 This IL enhanced the membrane dynamics and increased the membrane fluidity in LUV from liver lipids, presumably due to incorporation of the cation into the bilayer with the subsequent alignment parallel to lipid tails.…”

Section: Interactions With Separate Cellular Organelles and Moleculesmentioning

confidence: 99%

“…The presence of negatively charged lipids in the bilayer led to stronger interactions with the ILs. , In another study, C n MIm BF 4 ( n = 2, 4, 8) caused disturbance of 10,12-pentacosadiynoic acid (PCDA), PCDA-DMPC, and DPPC membranes in an amount- and alkyl side chain length-dependent manner . The strength of the interaction of C n MIm BF 4 ILs ( n = 2, 8, 10) with unilamellar vesicles (ULVs) from liver lipids depended on the alkyl side chain length . C 10 MIm BF 4 caused disorder of the lipid monolayer and shrinkage of multilamellar DPPC vesicles (MLV); it also increased their surface charge and had a disordering effect on lipid chains in MLVs at concentrations below the CMC .…”

Section: Mechanisms Of Biological Activity Of Ionic Liquids: State Of...mentioning

confidence: 99%

Mechanisms of Biological Effects of Ionic Liquids: From Single Cells to Multicellular Organisms

Egorova,

Kibardin,

Posvyatenko

et al. 2024

Chem. Rev.

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The review presents a detailed discussion of the evolving field studying interactions between ionic liquids (ILs) and biological systems. Originating from molten salt electrolytes to present multiapplication substances, ILs have found usage across various fields due to their exceptional physicochemical properties, including excellent tunability. However, their interactions with biological systems and potential influence on living organisms remain largely unexplored. This review examines the cytotoxic effects of ILs on cell cultures, biomolecules, and vertebrate and invertebrate organisms. Our understanding of IL toxicity, while growing in recent years, is yet nascent. The established findings include correlations between harmful effects of ILs and their ability to disturb cellular membranes, their potential to trigger oxidative stress in cells, and their ability to cause cell death via apoptosis. Future research directions proposed in the review include studying the distribution of various ILs within cellular compartments and organelles, investigating metabolic transformations of ILs in cells and organisms, detailed analysis of IL effects on proteins involved in oxidative stress and apoptosis, correlation studies between IL doses, exposure times and resulting adverse effects, and examination of effects of subtoxic concentrations of ILs on various biological objects. This review aims to serve as a critical analysis of the current body of knowledge on IL-related toxicity mechanisms. Furthermore, it can guide researchers toward the design of less toxic ILs and the informed use of ILs in drug development and medicine.

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Thermodynamics and structure of model bio-membrane of liver lipids in presence of imidazolium-based ionic liquids

Cited by 13 publications

References 85 publications

Cholesterol-Controlled Interaction of Ionic Liquids with Model Cellular Membranes

Cholesterol-Controlled Interaction of Ionic Liquids with Model Cellular Membranes

Modulation of Phase Behavior and Microscopic Dynamics in Cationic Vesicles by 1-Decyl-3-methylimidazolium Bromide

Mechanisms of Biological Effects of Ionic Liquids: From Single Cells to Multicellular Organisms

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