Thermodynamic study of poly(N-vinyl caprolactam) hydration at temperatures close to lower critical solution temperature

Tager, A.A.; Сафронов, А. П.; Sharina, S.V.; Galaev, Igor Yu.

doi:10.1007/bf00652769

Cited by 49 publications

(48 citation statements)

References 8 publications

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“…Compared to poly(vinylcaprolactam) (PVCL) of the same molecular weight and concentration, the T c of 5 is about 7 8C lower, [2,[14][15][16] although the monomer units are isomers. The attached ethylene group shields the vicinal polaric carbonyl group partially from the water phase and causes a higher hydrophobicity of the polymer.…”

Section: Resultsmentioning

confidence: 99%

“…[2,[14][15][16] To mimic this effect, we were encouraged to modify the industrially used NVP 1 with one additional ethyl substituent to achieve the same elemental composition as NVCL 2 ( Figure 1). In the present paper, we describe the synthesis of the ethyl-modified 3-ethyl-1-vinyl-2-pyrrolidone (3) and the preparation of its homopolymer and copolymers.…”

Section: Introductionmentioning

confidence: 99%

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3‐Ethylated N‐Vinyl‐2‐pyrrolidone with LCST Properties in Water

Trellenkamp

Ritter

2009

Macromol. Rapid Commun.

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The monomer 3-ethyl-1-vinyl-2-pyrrolidone (3) and the homopolymer poly(3-ethyl-1-vinyl-2-pyrrolidone) (5) have been synthesized. Polymer 5 is soluble in water and shows a critical temperature (T(c) ) of 27 °C. The presence of cyclodextrin causes a slight shift of the T(c) . The lower critical solution temperature (LCST) could be varied between 27 and 40 °C by copolymerization with N-vinyl-2-pyrrolidone. A linear correlation between the T(c) and the copolymer composition is observed.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

3‐Ethylated N‐Vinyl‐2‐pyrrolidone with LCST Properties in Water

Trellenkamp

Ritter

2009

Macromol. Rapid Commun.

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“…Poly(N-vinyl caprolactam) is a polymer which has a low critical solution tem-perature (LCST) in aqueous solution in the temperature range 32-35 0C (Tager et al, 1990). This temperature is quite acceptable for bioseparations.…”

Section: Resultsmentioning

confidence: 99%

Affinity thermoprecipitation of trypsin using soybean trypsin inhibitor conjugated with a thermo-reactive polymer, poly(N-vinyl caprolactam)

Galaev

Mattìasson

1992

Biotechnol Tech

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The soybean trypsin inhibitor conjugate with a thermo-reactive water soluble polymer, poly(N=vinyl caprolactam), was successfully used for thermally induced affinity precipitation of trypsin. The validity of the developed procedure was proven by a model separation of trypsin from dilute solution containing a large excess of bovine serum albumin.Affinity interactions are widely used in various strategies for purification of biomolecules. One of these strategies, affinity precipitation, discussed in detail in a recent review , utilizes hetero-bifunctional agents.One functional moiety of these agents interacts only with a desired biomolecule, and the other functional moiety precipitates when small changes are made in the properties of the medium. This causes specific precipitation of the affinity complex from the mixture. The ability of such heterobifunctional agents to precipitate on small changes of the properties of the medium must be rather conservative and be unchanged after binding with a desired biomolecule. Such conservatism is inherent mainly, if not exclusively, to polymers. The only polymer used to date for thermally induced affinity precipitation is poly(N-iso-propylacrylamide) or polyNIPAM (Monji and Hoffman, 1987, Ng~len and Luong, 1989, Pecs et al., 1991. This study presents the use of another thermo-reactive polymer, poly(N-vinyl caprolactam) (PVCL), as a ligand carrier for temperature induced affinity precipitation. Materials and MethodsChemicals N-Vinyl caprolactam was synthesized by reacting acetylene with caprolactam at 135-140 0C in an autoclave using potassium salt of caprolactam (5 % (w/w)) as a catalyst. The reaction proceeded for 2-3 h. The yield of N-vinyl caprolactam was 50-55 % after distillation at 81-830C and 1.5 torr. The purity of the monomer as determined by GLC was 98 % (Kononov et al., 1972). PVCL was produced by radical 353

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“…Upon further increase in surfactant concentration the amplitude of the low-temperature peak increases while that of the hightemperature peak vanishes. Phase separation transition in aqueous solutions of N-vinylcaprolactam-methacrylic acid copolymers of different compositions were studied at different polymer concentrations and pH (4)(5)(6)(7)(8)(9)(10)(11)(12). The transition parameters do not depend on polymer concentration and pH.…”

Section: Introductionmentioning

confidence: 99%

“…[5,9] The mixing enthalpy and entropy are negative. [9] This can be due to the hydrophobic hydration of the polymer.…”

Section: Introductionmentioning

confidence: 99%

Energetics of Cooperative Transitions of N‐Vinylcaprolactam Polymers in Aqueous Solutions

Dubovik

Махаева

Grinberg

et al. 2005

Macro Chemistry & Physics

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Thermodynamic study of poly(N-vinyl caprolactam) hydration at temperatures close to lower critical solution temperature

Cited by 49 publications

References 8 publications

3‐Ethylated N‐Vinyl‐2‐pyrrolidone with LCST Properties in Water

3‐Ethylated N‐Vinyl‐2‐pyrrolidone with LCST Properties in Water

Affinity thermoprecipitation of trypsin using soybean trypsin inhibitor conjugated with a thermo-reactive polymer, poly(N-vinyl caprolactam)

Energetics of Cooperative Transitions of N‐Vinylcaprolactam Polymers in Aqueous Solutions

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