Thermodynamic studies of partitioning behavior of cytochrome c in ionic liquid-based aqueous two-phase system

Lu, Yanmin; Lu, Wenjuan; Wang, Wei; Guo, Qi; Yang, Yanzhao

doi:10.1016/j.talanta.2011.06.058

Cited by 60 publications

(35 citation statements)

References 44 publications

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“…IL-based ABS have been used for the extraction of proteins and enzymes, while demonstrating to provide higher extraction efficiencies than the traditional PEG-based ABS [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34]. Alkylimidazolium-based ILs combined with phosphate-based salts were the most widely investigated phase-forming components of ABS [15].…”

Section: Introductionmentioning

confidence: 99%

Extraction and stability of bovine serum albumin (BSA) using cholinium-based Good's buffers ionic liquids

Taha

Quental

Correia

et al. 2015

Process Biochemistry

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Good's buffers ionic liquids (GB-ILs), composed of cholinium-based cations and Good's buffers anions, display self-buffering characteristics in the biological pH range, and their polarity and hydrophobicity can be easily tuned by a proper manipulation of their ions chemical structures. In this work, the extraction ability for bovine serum albumin (BSA) of aqueous biphasic systems (ABS) formed by polypropylene glycol 400 (PPG 400) and several GB-ILs was evaluated. ABS formed by PPG 400 and cholinium chloride ([Ch]Cl), GBs, and sucrose were also investigated for comparison purposes. It is shown that BSA preferentially migrates for the GB-IL-rich phase, with extraction efficiencies of 100%, achieved in a single-step. Dynamic light scattering, and circular dichroism (CD) and Fourier transform infrared (FTIR) spectroscopies were employed to evaluate the effect of the investigated cholinium-based GB-ILs on the BSA stability, and compared with results obtained for the respective GBs precursors, [Ch]Cl and sucrose, a well-known protein stabilizer. Molecular docking studies were also carried out to investigate on the binding sites of GB-IL ions to BSA. The experimental results confirm that BSA has a higher stability in GB-ILs than in any of the other compounds investigated.

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Section: Introductionmentioning

confidence: 99%

Extraction and stability of bovine serum albumin (BSA) using cholinium-based Good's buffers ionic liquids

Taha

Quental

Correia

et al. 2015

Process Biochemistry

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“…[6][7][8][9] Since the last century, phase separation or phase transformation in ionic liquid aqueous two-phase systems has been well known in the fields of analytical chemistry and separation science. 1,[10][11][12][13] Ionic liquid aqueous systems separate into two distinct phases, namely an ionic liquid-rich phase and ionic liquid-poor phase, when cooled below a certain temperature. 13 In our previous study, 8,14 the TRDP and TRDF behavior of ionic liquid aqueous two-phase systems (e.g., water-1-butyl-3-methylimidazolium chloride ([C4mim]Cl)-KOH, water-[C4mim]Cl-K2HPO4, and water-1-ethyl-3-methylimidazolium methylphosphonate ([C2mim]MP)-K2HPO4) showing phase separation characteristics were examined by feeding into a capillary tube.…”

Section: Introductionmentioning

confidence: 99%

Tube Radial Distribution Flow Separation in a Microchannel Using an Ionic Liquid Aqueous Two-Phase System Based on Phase Separation Multi-Phase Flow

et al. 2016

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Bright-field microscope setup with a CCD camera system 8,15 A bright-field microscope-CCD camera system was set up to examine the capillary tubes ( Supporting Information, Fig. S1). Ionic liquid aqueous two-phase systems were delivered into a capillary tube to achieve tube radial distribution flow (TRDF) or annular flow in a microspace. The phase diagram, viscosity of the phases, and TRDF image of the 1-butyl-3-methylimidazolium chloride and NaOH system were examined. The TRDF was formed with inner ionic liquid-rich and outer ionic liquid-poor phases in the capillary tube. The phase configuration was explained using the viscous dissipation principle. We also examined the distribution of rhodamine B in a three-branched microchannel on a microchip with ionic liquid aqueous two-phase systems for the first time.

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“…[1][2][3][4][5] For example, aqueous micellar solutions of specific non-ionic surfactants separate into two distinct phases when heated above a certain temperature (cloud point), i.e., a temperature-induced phase separation occurs. [6][7][8][9][10] One phase behaves as an almost surfactant-free aqueous solution (aqueous phase), whereas the other phase is a concentrated surfactant solution (surfactant-rich phase).…”

Section: Introductionmentioning

confidence: 99%

Investigation of Inner and Outer Phase Formation in Tube Radial Distribution Phenomenon Using Various Types of Mixed Solvent Solutions

et al. 2014

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When mixed solvent solutions, such as ternary water-hydrophilic/hydrophobic organic solvents, water-surfactant, water-ionic liquid, and fluorous-organic solvents are delivered into a microspace under laminar flow conditions, the solvent molecules are radially distributed in the microspace, generating inner and outer phases. This specific fluidic behavior is termed "tube radial distribution phenomenon" (TRDP). In this study, the factors influencing the formation of inner and outer phases in the TRDP using the above-mentioned mixed solvent solutions were investigated. We examined phase diagrams, viscosities of the two phases (upper and lower phases in a batch vessel), volume ratios of the phases, and bright-light or fluorescence photographs of the TRDP. When the difference in viscosities between the two phases was large (> approximately 0.73 mPa·s), the phase with the larger viscosity formed an inner phase regardless of the volume ratios, whereas when the difference was small (< approximately 0.42 mPa·s), the phase with the larger volume formed an inner phase. The TRDP using a water-surfactant mixed solution was also investigated in capillary chromatography based on TRDP.

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Thermodynamic studies of partitioning behavior of cytochrome c in ionic liquid-based aqueous two-phase system

Cited by 60 publications

References 44 publications

Extraction and stability of bovine serum albumin (BSA) using cholinium-based Good's buffers ionic liquids

Extraction and stability of bovine serum albumin (BSA) using cholinium-based Good's buffers ionic liquids

Tube Radial Distribution Flow Separation in a Microchannel Using an Ionic Liquid Aqueous Two-Phase System Based on Phase Separation Multi-Phase Flow

Investigation of Inner and Outer Phase Formation in Tube Radial Distribution Phenomenon Using Various Types of Mixed Solvent Solutions

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