Thermodynamic quantities for the dissociation equilibria of biologically important compounds. 4. The second acid dissociation of glucose 1-phosphoric acid

Ashby, Jack; Clarke, H. B.; Crook, E. M.; Datta, S. P.

doi:10.1042/bj0590203

Cited by 32 publications

(12 citation statements)

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“…In literature, the synthesis of α-glucose-1-phosphate (αGlc1P) from starch [10,11] or sucrose [12] and that of α-galactose-1-phosphate (αGal1P) from lactose [13,14] have previously been reported. A production process for βGlc1P has not yet been described in detail, but is similarly feasible using GH65 disaccharide phosphorylases from cheap substrates [15] briefly mention the production of βGlc1P from trehalose, using typical reaction conditions employed for αGlc1P [16]. In our hands, however, a crystalline product could not be obtained using these procedures.…”

Section: Introductioncontrasting

confidence: 38%

“…After this gel was dissolved in An alternative purification method was also investigated that might be more suitable for largescale operations. In literature, baker's yeast Saccharomyces cerevisiae is often used to metabolize sugar by-products in bioconversion reactions [30][31][32] while phosphate can be removed by chemical precipitation [16]. However, Yoon et al [32] have noted that trehalose is only partially hydrolyzed by baker's yeast.…”

Section: Purification Of β β β βGlc1pmentioning

confidence: 99%

“…After adjustment of the pH to > 8 with KOH, βGlc1P was precipitated by the addition of ethanol to form a transparent gel after overnight incubation at 4°C. This gel was In the second strategy, βGlc1P was purified by simultaneous removal of trehalose by E. coli trehalase (200 mL cell extract / L substrate) and glucose by baker's yeast (20 g/L) at 37°C during 24 h. Phosphate was precipitated by the addition of magnesium acetate and ammonia [16]. Biomass and precipitated phosphate were removed by filtration through a Seitz EKS filter, and βGlc1P was further purified by ethanol precipitation as described earlier.…”

Section: Purification and Crystallization Of β β β βGlc1pmentioning

confidence: 99%

“…Yeast biomass was removed by filtration. Phosphate was precipitated from the solution in the form of struvite (magnesium ammonium phosphate) as described by Asbhy et al [16]. Struvite can be recovered from the process and has potential applications as a slow-release fertilizer [33].…”

Section: Purification Of β β β βGlc1pmentioning

confidence: 99%

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Enzymatic production of β‐D‐glucose‐1‐phosphate from trehalose

Borght¹,

Desmet²,

Soetaert³

2010

Biotechnology Journal

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beta-D-Glucose-1-phosphate (beta Glc1P) is an efficient glucosyl donor for both enzymatic and chemical glycosylation reactions but is currently very costly and not available in large amounts. This article provides an efficient production method of beta Glc1P from trehalose and phosphate using the thermostable trehalose phosphorylase from Thermoanaerobacter brockii. At the process temperature of 60 C, Escherichia coli expression host cells are lysed and cell treatment prior to the reaction is, therefore, not required. In this way, the theoretical maximum yield of 26% could be easily achieved. Two different purification strategies have been compared, anion exchange chromatography or carbohydrate removal by treatment with trehalase and yeast, followed by chemical phosphate precipitation. In a next step, beta Glc1P was precipitated with ethanol but this did not induce crystallization, in contrast to what ist observed with other glycosylphosphates. After conversion of the product to its cyclohexylammonium salt, however, crystals could be readily obtained. Although both purification methods were quantitative (>99% recovery), a large amount of product (50%) was lost during crystallization. Nevertheless, a production process for crystalline beta Glc1P is now available from the cheap substrates trehalose and inorganic phosphate

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Section: Introductioncontrasting

confidence: 38%

Section: Purification Of β β β βGlc1pmentioning

confidence: 99%

Section: Purification and Crystallization Of β β β βGlc1pmentioning

confidence: 99%

Section: Purification Of β β β βGlc1pmentioning

confidence: 99%

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Enzymatic production of β‐D‐glucose‐1‐phosphate from trehalose

Borght¹,

Desmet²,

Soetaert³

2010

Biotechnology Journal

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“…A reaction mixture (150 L) containing 195 mM of trehalose and TPase (3.42 U/mmol for trehalose) in 500 mM sodium phosphate buffer (pH 7.0) was incubated at 55°C for 72 h. The purification of b-G1P as done by the method of Ashby et al 20 and we obtained 1580 g of b-G1PAE2Na.…”

Section: Preparation Of B-g1pmentioning

confidence: 99%

Enzymatic synthesis of a 2-O-α-d-glucopyranosyl cyclic tetrasaccharide by kojibiose phosphorylase

Watanabe

Higashiyama

Aga

et al. 2005

Carbohydrate Research

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Biochemical Studies on Post‐Ripening of Banana

Yang

1958

J Chinese Chemical Soc

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Some fundamental studies were undertaken in order to get some insight into the process of post-ripening of banana by studying the 'changes in carbohyrkare metabolism and the changes in respiratory mechanism during the c o m e of banana ripening.During the course of banana ripening, it is concluded that sucrose is synthesized first directly from starch and subsequently hydrolyzed into glucose and fructose in the post-climacteric stage. In this process phosphorylase appears to be the main enzyme involved in thc breakdown of starch. 2.During the course of post-ripening, water content of the flesh increases gradually. Soluble pectin kcteases four times in the climacteric stage, and then decreases.No peroxidase acLivity is detected through each stage of ripening, but considerable *mcensity of catalase activity is detected. The catalase activity in pre-climacteric banana is stronger than that in the climacteric. 4.The entire terminal oxidst? of preclimacteric banana flesh 'is cyanideresistant, but as the climacteric stage reaches, one-fifth of it becomes cyanide-sensitive. Malonate poisoning appeares to occur to a very little extent.Chrate acts as a substrate toward the respiraLory system of preclimacteric tissue, but as an inhibitor toward that of ripe banana. An interpretation is made on thesz effe$s of citrate.6. With the aid of the uncoupling agent, 2,PdinitrophenoI, it is shown that the respiratory rate of the preclimacteric but' not of the climacteric banana flesh is limited by the capacity of the phosphate transfer system. ATP-ase develops during the course of ripening and appeares to be a factor which put forth the respiratory increase in the climacteric stage. This behavior is belived to be the general feature of the catabolism of reserve substances like starch. 1.Tannin content remains unchanged. 3. . 7Banana i s one of the most abundant fruits in Taiwan There have been many studies on the post-harvcst physiology of fruits('lg), but only a few on biochemistry, I n this investigation, the authors have attempted to get some insight into the process of post-ripening of banana by studying the changes in carbohydrate metabolism and the changes in respiratory mechanism during the course of banana ripening, M a t e r i a l s and M e t h o d s SampleT h e species of banana used throughout this experiment was Musa sapientzrm, L., grown at Suei-Li-Kon (7k+g&) in Taiwan. Respiration measurementA stream of air, freed from carbon dioxide by passing through sodium hydroxide solution, humidified by passing through water, was led over the banana fruit in a respiratory chamber, and then through an absorption tower containing a known quantity of 0.5 N Na0,H.After 'addition of BaCI, solution, the excess alkali was titrated with 0.1 N HCI, using phenolphthalein as indicator.thus determined.T h e amount of CO, evolved was Analysis of the constituent About 30 g of the banana flesh was blended in 95;g ethanol with a wariiig bIendor to destroy the enzymes and to avoid any change of the constituents, and anaIyzed by the usual method...

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Thermodynamic quantities for the dissociation equilibria of biologically important compounds. 4. The second acid dissociation of glucose 1-phosphoric acid

Cited by 32 publications

References 4 publications

Enzymatic production of β‐D‐glucose‐1‐phosphate from trehalose

Enzymatic production of β‐D‐glucose‐1‐phosphate from trehalose

Enzymatic synthesis of a 2-O-α-d-glucopyranosyl cyclic tetrasaccharide by kojibiose phosphorylase

Biochemical Studies on Post‐Ripening of Banana

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