Thermodynamic profiling during irreversible electroporation in porcine liver and pancreas: a case study series

Agnass, Pierre; Veldhuisen, Eran van; Vogel, Jantien A.; Kok, H. Petra; Keijzer, Mark J de; Schooneveldt, G.; Haan, Lianne R. de; Klaessens, John; Scheffer, Hester J.; Meijerink, Martijn R.; Lienden, Krijn P. van; Gulik, Thomas M. Van; Heger, Michal; Crezee, J.; Besselink, Marc G.

doi:10.18053/jctres.05.202003.004

Cited by 8 publications

(5 citation statements)

References 40 publications

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“…• a significant temperature difference compared to 37 • C, allowing us to demonstrate the capability of HyCHEED to operate at different temperatures; • the likelihood of occurrence of 46 • C during typical IRE treatment [9][10][11];…”

Section: Choice and Evaluation Of Pulse Settings And Target Temperaturesmentioning

confidence: 99%

“…At these temperatures, clinical IRE setting was applied to the TV consisting of a series of 90 pulses with a pulse duration of 90 µs and an electric-field strength (E [V•cm −1 ]) of 1250 V•cm −1 [11,29,31]. To examine the influence of the T target on the TV during IRE, additional IRE setting was selected in which the E value was reduced to 500 V•cm −1 while maintaining the pulse duration and number.…”

Section: Choice and Evaluation Of Pulse Settings And Target Temperaturesmentioning

confidence: 99%

“…Despite the fact that IRE was initially introduced as a non-thermal technique, the application of repetitive high-intensity electrical pulses in the target organs, inevitably leads to increased temperatures during ablation [8][9][10][11]. The produced thermal effects could have the potential to enhance the effectiveness of IRE treatment, since mild hyperthermia and thermal ablation are known to have therapeutic effects.…”

Section: Introductionmentioning

confidence: 99%

“…We chose IPE instead of IRE effect to distinguish between only the permeabilization effect, and the permeabilization and the thermal effects jointly produced by IRE. For higher temperature levels (T ≥ 50 • C [8,20,21]), even direct thermal damage could occur, which can result in thermal ablation, especially in the vicinity of the needle electrodes [8][9][10][11]. However, it remains unclear to what extent the thermal effects of IRE contribute to tissue ablation.…”

Section: Introductionmentioning

confidence: 99%

“…Establishing the contribution of thermal effects requires performing pre-clinical IRE experiments while maintaining a well-defined, stable temperature. The latter is challenging, as the very process of IRE can cause an unpredictable temperature rise [8][9][10][11]20], which needs to be dealt with to achieve stable experimental conditions. Several studies developed setups that aimed at maintaining the temperature of the target volume at a specific target temperature (T target [ • C]) during IRE [27,28].…”

Section: Introductionmentioning

confidence: 99%

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HyCHEED System for Maintaining Stable Temperature Control during Preclinical Irreversible Electroporation Experiments at Clinically Relevant Temperature and Pulse Settings

Agnass

Rodermond

Zweije

et al. 2020

Sensors

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Electric permeabilization of cell membranes is the main mechanism of irreversible electroporation (IRE), an ablation technique for treatment of unresectable cancers, but the pulses also induce a significant temperature increase in the treated volume. To investigate the therapeutically thermal contribution, a preclinical setup is required to apply IRE at desired temperatures while maintaining stable temperatures. This study’s aim was to develop and test an electroporation device capable of maintaining a pre-specified stable and spatially homogeneous temperatures and electric field in a tumor cell suspension for several clinical-IRE-settings. A hydraulically controllable heat exchange electroporation device (HyCHEED) was developed and validated at 37 °C and 46 °C. Through plate electrodes, HyCHEED achieved both a homogeneous electric field and homogenous-stable temperatures; IRE heat was removed through hydraulic cooling. IRE was applied to 300 μL of pancreatic carcinoma cell suspension (Mia PaCa-2), after which cell viability and specific conductivity were determined. HyCHEED maintained stable temperatures within ±1.5 °C with respect to the target temperature for multiple IRE-settings at the selected temperature levels. An increase of cell death and specific conductivity, including post-treatment, was found to depend on electric-field strength and temperature. HyCHEED is capable of maintaining stable temperatures during IRE-experiments. This provides an excellent basis to assess the contribution of thermal effects to IRE and other bio-electromagnetic techniques.

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Section: Choice and Evaluation Of Pulse Settings And Target Temperaturesmentioning

confidence: 99%

Section: Choice and Evaluation Of Pulse Settings And Target Temperaturesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

HyCHEED System for Maintaining Stable Temperature Control during Preclinical Irreversible Electroporation Experiments at Clinically Relevant Temperature and Pulse Settings

Agnass

Rodermond

Zweije

et al. 2020

Sensors

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Recurrent Colorectal Liver Metastases in the Liver Remnant After Major Liver Surgery—IRE as a Salvage Local Treatment When Resection and Thermal Ablation are Unsuitable

Hitpaß

Distelmaier

Neumann

et al. 2021

Cardiovasc Intervent Radiol

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Purpose To examine the safety and short-term oncologic outcomes of computer-tomography-guided (CT-guided) irreversible electroporation (IRE) of recurrent, irresectable colorectal liver metastases (CRLM) after major hepatectomy deemed unsuitable for thermal ablation. Patients and Methods Twenty-three patients undergoing CT-guided IRE of recurrent CRLM after major hepatectomy were included in this study. All tumors were located adjacent to sole remaining intrahepatic blood vessels and bile ducts, precluding thermal ablation. Patients underwent systematic clinical and imaging follow-up, including magnetic resonance imaging of the liver at 1-month and 3-month intervals thereafter. Time to local and intrahepatic tumor progression within 12 and 36 months and associated risk factors were assessed using Kaplan Meier and Cox regression analysis, respectively. Results Complete ablation with a safety margin of at least 0.5 cm was achieved in 22/23 (95.6%) patients. No vessel injury or thrombosis occurred. Five patients developed moderate biliary stenosis after a median of 4 weeks, without requiring treatment. Local tumor-progression-free rates within 12/36 months were 64%/57.4%, respectively. Intrahepatic-progression-free rate within 12/36 months was 36.4%/19.5%, respectively. Five (23%) patients were tumor-free at the end of follow-up. Multivariate Cox regression analysis did not show any association between local tumor-progression-free rates and patient age, target tumor size, primary tumor side or synchronicity of liver metastases. Conclusion In this highly selected patient population with local recurrences of CRLM after major surgery, IRE was shown to be a safe salvage treatment option when thermal ablation is unsuitable.

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Optimization of laser dosimetry based on patient-specific anatomical models for the ablation of pancreatic ductal adenocarcinoma tumor

Namakshenas

Matteo

Bianchi

et al. 2023

Sci Rep

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Laser-induced thermotherapy has shown promising potential for the treatment of unresectable primary pancreatic ductal adenocarcinoma tumors. Nevertheless, heterogeneous tumor environment and complex thermal interaction phenomena that are established under hyperthermic conditions can lead to under/over estimation of laser thermotherapy efficacy. Using numerical modeling, this paper presents an optimized laser setting for Nd:YAG laser delivered by a bare optical fiber (300 µm in diameter) at 1064 nm working in continuous mode within a power range of 2–10 W. For the thermal analysis, patient-specific 3D models were used, consisting of tumors in different portions of the pancreas. The optimized laser power and time for ablating the tumor completely and producing thermal toxic effects on the possible residual tumor cells beyond the tumor margins were found to be 5 W for 550 s, 7 W for 550 s, and 8 W for 550 s for the pancreatic tail, body, and head tumors, respectively. Based on the results, during the laser irradiation at the optimized doses, thermal injury was not evident either in the 15 mm lateral distances from the optical fiber or in the nearby healthy organs. The present computational-based predictions are also in line with the previous ex vivo and in vivo studies, hence, they can assist in the estimation of the therapeutic outcome of laser ablation for pancreatic neoplasms prior to clinical trials.

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Thermodynamic profiling during irreversible electroporation in porcine liver and pancreas: a case study series

Cited by 8 publications

References 40 publications

HyCHEED System for Maintaining Stable Temperature Control during Preclinical Irreversible Electroporation Experiments at Clinically Relevant Temperature and Pulse Settings

HyCHEED System for Maintaining Stable Temperature Control during Preclinical Irreversible Electroporation Experiments at Clinically Relevant Temperature and Pulse Settings

Recurrent Colorectal Liver Metastases in the Liver Remnant After Major Liver Surgery—IRE as a Salvage Local Treatment When Resection and Thermal Ablation are Unsuitable

Optimization of laser dosimetry based on patient-specific anatomical models for the ablation of pancreatic ductal adenocarcinoma tumor

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