Thermocontrolled Reversible Enzyme Complexation-Inactivation-Protection by Poly(N-acryloyl glycinamide)

Semenyuk, Pavel I.; Kurochkina, Lidia P.; Mäkinen, Lauri; Muronetz, Vladimir I.; Hietala, Sami

doi:10.3390/polym13203601

Cited by 5 publications

(10 citation statements)

References 39 publications

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“…Hietala et al. obtained similar results by capturing lysozymes using polymer chains at low temperatures and releasing them at high temperatures [53] …”

Section: Current Approaches To Regulate Enzyme Activity For Biomedica...mentioning

confidence: 77%

“…Hietala et al obtained similar results by capturing lysozymes using polymer chains at low temperatures and releasing them at high temperatures. [53] Thermoresponsive hydrogels also have great potential for controlling enzymatic activity. Recently, Claaßen et al described a temperature-dependent poly(N-acryloyl glycinamide) (pNAGA) hydrogel that could regulate the enzymatic activity of immobilized Bacillus megaterium transaminase (BmTA) (Figure 7A).…”

Section: Heatmentioning

confidence: 99%

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Advanced Strategies of Enzyme Activity Regulation for Biomedical Applications

2022

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Enzymes are important macromolecular biocatalysts that accelerate chemical and biochemical reactions in living organisms. Most human diseases are related to alterations in enzyme activity. Moreover, enzymes are potential therapeutic tools for treating different diseases, such as cancer, infections, and cardiovascular and cerebrovascular diseases. Precise remote enzyme activity regulation provides new opportunities to combat diseases. This review summarizes recent advances in the field of enzyme activity regulation, including reversible and irreversible regulation. It also discusses the mechanisms and approaches for on‐demand control of these activities. Furthermore, a range of stimulus‐responsive inhibitors, polymers, and nanoparticles for regulating enzyme activity and their prospective biomedical applications are summarized. Finally, the current challenges and future perspectives on enzyme activity regulation are discussed.

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“…Hietala et al. obtained similar results by capturing lysozymes using polymer chains at low temperatures and releasing them at high temperatures [53] …”

Section: Current Approaches To Regulate Enzyme Activity For Biomedica...mentioning

confidence: 77%

Section: Heatmentioning

confidence: 99%

Advanced Strategies of Enzyme Activity Regulation for Biomedical Applications

2022

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“…iMAPA-HA exhibits a phase transition in aqueous solutions becoming soluble at high temperatures with a UCST response. Semenyuk et al proposed the use of poly(N-acryloyl glycinamide) (PNAGA), a UCST-responsive polymer soluble at high temperatures [79]. Figure 9 describes the technology proposed to deliver an enzyme based on a UCST thermo responsive polymer.…”

Section: Thermo-responsivementioning

confidence: 99%

Single and Multiple Stimuli-Responsive Polymer Particles for Controlled Drug Delivery

2022

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Polymers that can change their properties in response to an external or internal stimulus have become an interesting platform for drug delivery systems. Polymeric nanoparticles can be used to decrease the toxicity of drugs, improve the circulation of hydrophobic drugs, and increase a drug’s efficacy. Furthermore, polymers that are sensitive to specific stimuli can be used to achieve controlled release of drugs into specific areas of the body. This review discusses the different stimuli that can be used for controlled drug delivery based on internal and external stimuli. Internal stimuli have been defined as events that evoke changes in different characteristics, inside the body, such as changes in pH, redox potential, and temperature. External stimuli have been defined as the use of an external source such as light and ultrasound to implement such changes. Special attention has been paid to the particular chemical structures that need to be incorporated into polymers to achieve the desired stimuli response. A current trend in this field is the incorporation of several stimuli in a single polymer to achieve higher specificity. Therefore, to access the most recent advances in stimuli-responsive polymers, the focus of this review is to combine several stimuli. The combination of different stimuli is discussed along with the chemical structures that can produce it.

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“…This is a strong argument in favor of using weak polyelectrolytes such as poly(acrylic acid) or other polycarboxylates and polyamines for immobilization. However, such destructive action might be reversible, suggesting a useful tool for enzymatic activity manipulation; controlled binding and release of the enzyme enables control of its activity [ 50 , 51 ].…”

Section: Effect Of Immobilization On Enzyme Activitymentioning

confidence: 99%

Polyelectrolytes for Enzyme Immobilization and the Regulation of Their Properties

2022

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In this review, we considered aspects related to the application of polyelectrolytes, primarily synthetic polyanions and polycations, to immobilize enzymes and regulate their properties. We mainly focused on the description of works in which polyelectrolytes were used to create complex and unusual systems (self-regulated enzyme–polyelectrolyte complexes, artificial chaperones, polyelectrolyte brushes, layer-by-layer immobilization and others). These works represent the field of “smart polymers”, whilst the trivial use of charged polymers as carriers for adsorption or covalent immobilization of proteins is beyond the scope of this short review. In addition, we have included a section on the molecular modeling of interactions between proteins and polyelectrolytes, as modeling the binding of proteins with a strictly defined, and already known, spatial structure, to disordered polymeric molecules has its own unique characteristics.

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Thermocontrolled Reversible Enzyme Complexation-Inactivation-Protection by Poly(N-acryloyl glycinamide)

Cited by 5 publications

References 39 publications

Advanced Strategies of Enzyme Activity Regulation for Biomedical Applications

Advanced Strategies of Enzyme Activity Regulation for Biomedical Applications

Single and Multiple Stimuli-Responsive Polymer Particles for Controlled Drug Delivery

Polyelectrolytes for Enzyme Immobilization and the Regulation of Their Properties

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