Thermococcus stetteri sp. nov., a New Extremely Thermophilic Marine Sulfur-Metabolizing Archaebacterium

Miroshnichenko, M. L.; Bonch-Osmolovskaya, E. A.; Neuner, Annemarie; Kostrikina, N. A.; Chernych, N.A.; Alekseev, V.A.

doi:10.1016/s0723-2020(89)80071-2

Cited by 100 publications

(33 citation statements)

References 22 publications

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“…Thermococcus radiotolerans, with its gamma-irradiation tolerance, has an a Determined at least in triplicate for cells grown in poor SMES medium and rich MAYTP medium. Swimming speeds at temperatures other than 85°C may be found in unusual physiology (27), while Thermococcus stetteri shows an unusually high maximum growth temperature and has been described as "nonmotile or motile with a polar tuft of flagella" (28). Finally, Thermococcus thioreducens was chosen due to its description as an obligate S 0 reducer (29).…”

Section: Methodsmentioning

confidence: 99%

The Temperature Gradient-Forming Device, an Accessory Unit for Normal Light Microscopes To Study the Biology of Hyperthermophilic Microorganisms

Mora

Bellack

Ugele

et al. 2014

Appl Environ Microbiol

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bTo date, the behavior of hyperthermophilic microorganisms in their biotope has been studied only to a limited degree; this is especially true for motility. One reason for this lack of knowledge is the requirement for high-temperature microscopy-combined, in most cases, with the need for observations under strictly anaerobic conditions-for such studies. We have developed a custom-made, low-budget device that, for the first time, allows analyses in temperature gradients up to 40°C over a distance of just 2 cm (a biotope-relevant distance) with heating rates up to ϳ5°C/s. Our temperature gradient-forming device can convert any upright light microscope into one that works at temperatures as high as 110°C. Data obtained by use of this apparatus show how very well hyperthermophiles are adapted to their biotope: they can react within seconds to elevated temperatures by starting motility-even after 9 months of storage in the cold. Using the temperature gradient-forming device, we determined the temperature ranges for swimming, and the swimming speeds, of 15 selected species of the genus Thermococcus within a few months, related these findings to the presence of cell surface appendages, and obtained the first evidence for thermotaxis in Archaea.

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Section: Methodsmentioning

confidence: 99%

The Temperature Gradient-Forming Device, an Accessory Unit for Normal Light Microscopes To Study the Biology of Hyperthermophilic Microorganisms

Mora

Bellack

Ugele

et al. 2014

Appl Environ Microbiol

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“…The phylogenetic position of strain KOD1 was located within the genus Thermococcus. With the se-quences we applied, strain KOD1 clustered with T. peptonophilus (González et al 1995) and T. stetteri (99%) (Miroshnichenko et al 1989). When the maximum-likelihood method was applied, the phylogenetic position of several strains changed, but the clustering among T. peptonophilus, T. stetteri and strain KOD1 was unaltered.…”

Section: Phylogenetic Analysis Of 16s Ribosomal Dna Sequencesmentioning

confidence: 99%

“…However, the %hybridization between strain KOD1 and T. stetteri was low (13%). Further, T. stetteri JCM8559 T (=DSM 5262 T ) grows only in the presence of elemental sulfur and not with free amino acids (Miroshnichenko et al 1989). Strain KOD1 can grow without elemental sulfur in the presence of starch, maltooligosaccharides and pyruvate.…”

Section: Comparison With T Peptonophilus and T Stetterimentioning

confidence: 99%

Description ofThermococcus kodakaraensissp. nov., a well studied hyperthermophilic archaeon previously reported asPyrococcussp. KOD1

Atomi

Fukui²,

Kanai³

et al. 2004

Archaea

271

186

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Summary A hyperthermophilic archaeal strain, KOD1, isolated from a solfatara on Kodakara Island, Japan, has previously been reported as Pyrococcus sp. KOD1. However, a detailed phylogenetic tree, made possible by the recent accumulation of 16S rRNA sequences of various species in the order Thermococcales, indicated that strain KOD1 is a member of the genus Thermococcus. We performed DNA-DNA hybridization tests against species that displayed high similarity in terms of 16S ribosomal DNA sequences, including Thermococcus peptonophilus and Thermococcus stetteri. Hybridization results and differences in growth characteristics and substrate utilization differentiated strain KOD1 from T. peptonophilus and T. stetteri at the species level. Our results indicate that strain KOD1 represents a new species of Thermococcus, which we designate as Thermococcus kodakaraensis KOD1 sp. nov.

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“…These features of fermentative-type metabolism are similar to those displayed by members of the Thermococcales, most of which are capable of growth using peptides in the presence or absence of S! (Zillig et al, 1983 ;Fiala & Stetter, 1986 ;Miroshnichenko et al, 1989 ;Pledger & Baross, 1989Neuner et al, 1990 ;Gonzalez et al, 1995Gonzalez et al, , 1998Godfroy et al, 1996Godfroy et al, , 1997Antoine et al, 1997 ;Canganella et al, 1997). The major core lipids of the new isolate were tetraether and diether lipids, which are the most predominant core lipids in the Thermococcales (Zillig et al, 1983 ;Pledger & Baross, 1991 ;Godfroy et al, 1996Godfroy et al, , 1997Gonzalez et al, 1998), and the 16S rDNA sequence was most closely related to those of members of the Thermococcales (Fig.…”

Section: Discussionmentioning

confidence: 87%

Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney.

Takai¹,

Sugai²,

Itoh³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

167

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Thermococcus stetteri sp. nov., a New Extremely Thermophilic Marine Sulfur-Metabolizing Archaebacterium

Cited by 100 publications

References 22 publications

The Temperature Gradient-Forming Device, an Accessory Unit for Normal Light Microscopes To Study the Biology of Hyperthermophilic Microorganisms

The Temperature Gradient-Forming Device, an Accessory Unit for Normal Light Microscopes To Study the Biology of Hyperthermophilic Microorganisms

Description ofThermococcus kodakaraensissp. nov., a well studied hyperthermophilic archaeon previously reported asPyrococcussp. KOD1

Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney.

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