Thermochemolysis of genetically different soil humic acids and their fractions obtained by tandem size exclusion chromatography–polyacrylamide gel electrophoresis

Sáiz‐Jiménez, Cesáreo; Hermosín, Bernardo; Trubetskaya, O. E.; Reznikova, O. I.; Afanas'eva, G. V.; Trubetskoj, Oleg

doi:10.1016/j.geoderma.2005.03.001

Cited by 28 publications

(15 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this paper the HA and its fractions A, B, and CþD obtained by SEC-PAGE were studied in the scan range 1000-4000 amu, considered the most informative, because this range provides insights on the original components and their chemical structure. 25 The chromatograms of the thermochemolysates of HA and its fractions A, B, and CþD are displayed in Fig. 1.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Surface-Enhanced Raman Spectroscopy of Chernozem Humic Acid and Their Fractions Obtained by Coupled Size Exclusion Chromatography—Polyacrylamide Gel Electrophoresis (SEC-PAGE)

et al. 2006

Self Cite

View full text Add to dashboard Cite

A humic acid extracted from a chernozem soil was fractionated combining size exclusion chromatography and polyacrylamide electrophoresis (SEC-PAGE). Three fractions named A, B, and CþD, with different electrophoretic mobilities and molecular sizes (MS), were obtained and subsequently characterized by thermochemolysis and surface-enhanced Raman spectroscopy (SERS). The data confirmed that fraction A, with the higher MS, was more aliphatic than fractions B and CþD and, in turn, fractions with lower MS (B and CþD) denoted an enrichment in lignin residues. These structural features explain conformational changes when varying the pH in the humic fraction A and indicated that combination of the two techniques is a good approach for characterizing humic substances.

show abstract

Section: Resultsmentioning

confidence: 99%

“…3b), which could be related to the higher amount of lignin products in this fraction as revealed by electrophoresis and thermochemolysis data. 18,25 The molecular matrices forming part of this fraction are more open at neutral pH, as deduced from their different composition. The difference spectrum (Fig.…”

mentioning

confidence: 99%

Surface-Enhanced Raman Spectroscopy of Chernozem Humic Acid and Their Fractions Obtained by Coupled Size Exclusion Chromatography—Polyacrylamide Gel Electrophoresis (SEC-PAGE)

et al. 2006

Self Cite

View full text Add to dashboard Cite

show abstract

“…Trubetskoj et al (1997) used size-exclusion chromatography in the presence of concentrated urea for collecting the HA fractions with different electrophoretic behavior. They have applied this method for the investigation of soil HAs (Trubetskoj et al 1997(Trubetskoj et al , 1999(Trubetskoj et al , 2010Trubetskaya et al 1997Trubetskaya et al , 2002Trubetskaya et al , 2007Trubetskaya et al , 2011Aguer et al 2001;Saiz-Jimenez et al 2006;Sanchez-Cortes et al 2006;Richard et al 2007;Francioso et al 2009). This method is advantageous because a sufficient amount of the fractions with different electrophoretic behavior could be collected for characterization.…”

Section: Infrared Spectroscopy Of the Fractionsmentioning

confidence: 99%

“…Trubetskoj et al (1997) developed a method for the fractionation of soil HAs based on the combined use of size-exclusion chromatography and PAGE in the presence of concentrated urea. They have been applying this method to the investigation of soil HAs (Trubetskaya et al 1998(Trubetskaya et al , 2002(Trubetskaya et al , 2011Trubetskoj et al 1999;Aguer et al 2001;Saiz-Jimenez et al 2006;Sanchez-Cortes et al 2006;Guyot et al 2007;Richard et al 2007;Francioso et al 2009). The advantage of this method is that it allows one to collect a sufficient amount of the fractions with different electrophoretic behavior for characterization.…”

Section: Introductionmentioning

confidence: 99%

Fractionation of soil humic acids by preparative polyacrylamide gel electrophoresis in the presence of concentrated urea

Karim

Aoyama

2013

Soil Science and Plant Nutrition

View full text Add to dashboard Cite

To separate soil humic acids (HAs) into their constituents and characterize them, polyacrylamide gel electrophoresis (PAGE) was carried out in the presence of 7 M urea using a preparative electrophoresis system. Two types of soil HAs were fractionated into nine fractions by PAGE. The dark-colored constituents were recovered from the electrophoretic fractions by precipitation on acidification, and the brown-colored constituents dissolved in the acidic solution of fast-moving fractions were recovered by adsorption onto DAX-8 resin. High-performance size-exclusion chromatography (HPSEC) confirmed that the constituents of the HAs were separated based on their molecular size by PAGE. The dark-colored constituents exhibited higher degrees of humification than did the corresponding unfractionated HAs, except for the constituents remaining in the electrophoretic gels at the end of electrophoresis. Diffuse reflectance infrared spectroscopy revealed that the chemical properties of the dark-colored constituents changed regularly: the content of carboxyl groups decreased and the proportions of proteinous, aliphatic and polysaccharide moieties increased with increasing molecular size. The humification degrees of the constituents adsorbed onto DAX-8 resin were considerably lower than those of the corresponding unfractionated HAs. The chemical properties of the DAX-8-adsorbed constituents were different from those of the dark-colored constituents. Observation of electrophoretic fractions under blue light (470 nm) and HPSEC with fluorescence detection at an excitation wavelength of 460 nm and an emission wavelength of 520 nm showed that green fluorescent substances were largely concentrated in the smallest molecular size fractions and were partitioned into both the dark-colored precipitates and DAX-8-adsorbed fractions. The proportion of organic carbon recovered by precipitation and adsorption onto DAX-8 resin was 45-63%, indicating that substantial parts of the HA constituents were missing. The unrecovered constituents were considered to be acid-soluble, nearly colorless substances. The dissociation of the acid-soluble constituents from the acid-insoluble dark-colored constituents during the preparative PAGE of soil HAs was ascribed to disruption of hydrogen bonding and hydrophobic interactions caused by concentrated urea.

show abstract

“…HA isolated from other organic residues such as urban waste compost (Campitelli et al, 2006) and sewage sludge (Canellas et al, 2001) usually exhibit higher H/C ratio. Besides many studies, the process of organic matter stabilization still remains unclear (Saiz-Jimenez et al, 2006). Despite that, many authors believe that the chemical and biochemical reactions that occur during humification transform the organic matter through stabilization of aromatic structures, leading to a relative enrichment of aromatic carbon and selective preservation of alkyl carbon of soil biopolymers resistant to decomposition, such as cutins and suberins (Zech et al, 1997).…”

Section: Resultsmentioning

confidence: 99%

Humic substances isolated from residues of sugar cane industry as root growth promoter

Busato¹,

Zandonadi²,

Dobbss³

et al. 2010

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

Plant growth promoting substances are widely used in modern agriculture. Several products in the market are humic substances isolated from different sources. The filter cake, a residue of sugar production, is a rich and renewable source of organic matter and these characteristics place the filter cake as a possible source of plant growth promoting substances. Humic acids (HA) from filter cake were characterized, and their effects as root growth promoters were evaluated. Chemical features of the HA were evaluated through elemental composition, acidic functional groups, E 4 /E 6 ratio and infrared spectroscopy analyzes. The biological activity of the HA was assessed using root architecture parameters and the P-type H + -ATPase activity. The lateral root development was directly related to the stimulation of plasma membrane ATPase activity. The ability of HA to promote root development indicate that HA from filter cake can be used as environmental plant growth stimulators. Key words: H + -ATPase, Brazil, industrial residues, physiological effects, bioactivity Substâncias húmicas isoladas de resíduos da indústria da cana-de-açúcar como promotoras de crescimento radicularRESUMO: Substâncias promotoras do crescimento vegetal são amplamente utilizadas na agricultura moderna. Existem vários produtos no mercado, muitos dos quais são substâncias húmicas isoladas de diferentes fontes. A torta de filtro, um resíduo da produção do açúcar, é uma fonte rica e renovável de matéria orgânica e essas características a tornam uma possível fonte de substâncias promotoras do crescimento vegetal. Ácidos húmicos (AH) da torta de filtro foram caracterizados, e foi avaliado seu efeito como promotor de crescimento radicular. As características químicas dos AH foram avaliadas por meio da composição elementar, grupos funcionais ácidos, relação E 4 /E 6 e espectroscopia de infravermelho. A atividade biológica dos AH foi acessada avaliando-se a arquitetura radicular e a atividade da H + -ATPase de membrana plasmática. O desenvolvimento de raízes laterais foi diretamente relacionado ao estímulo da atividade da H + -ATPase. A habilidade dos AH em promover o desenvolvimento radicular indica que AH extraídos da torta de filtro podem ser utilizados como estimuladores do crescimento de plantas.

show abstract

Thermochemolysis of genetically different soil humic acids and their fractions obtained by tandem size exclusion chromatography–polyacrylamide gel electrophoresis

Cited by 28 publications

References 34 publications

Surface-Enhanced Raman Spectroscopy of Chernozem Humic Acid and Their Fractions Obtained by Coupled Size Exclusion Chromatography—Polyacrylamide Gel Electrophoresis (SEC-PAGE)

Surface-Enhanced Raman Spectroscopy of Chernozem Humic Acid and Their Fractions Obtained by Coupled Size Exclusion Chromatography—Polyacrylamide Gel Electrophoresis (SEC-PAGE)

Fractionation of soil humic acids by preparative polyacrylamide gel electrophoresis in the presence of concentrated urea

Humic substances isolated from residues of sugar cane industry as root growth promoter

Contact Info

Product

Resources

About