Thermochemistry of the reaction { phospho enol pyruvate(aq)+d -erythrose 4-phosphate(aq) + H2 O(l) = 2-dehydro-3-deoxy- d - arabino -heptonate 7-phosphate(aq) + phosphate(aq) }

Thermodynamics of the hydrolysis reactions of nitriles

“…These estimates of possible systematic error are combined in quadrature together with the statistical uncertainty in the measured value of D r H m (cal) expressed as one estimated standard deviation of the mean, to obtain combined standard uncertainties [9] for each result. These combined standard uncertainties are then multiplied by two to arrive at the final results from our experiments: D r H m (cal) = À(76.1 ± 0.9) kJ AE mol À1 for reaction (1); D r H m (cal) = À(88.1 ± 0.8) kJ AE mol À1 for reaction (2); D r H m (cal) = À(90.7 ± 0.8) kJ AE mol À1 for reaction (3); D r H m (cal) = À(81.0 ± 1.6) kJ AE mol À1 for reaction (4); D r H m (cal) = À(82.6 ± 1.4) kJ AE mol À1 for reaction (5); and D r H m (cal) = À(97.6 ± 1.1) kJ AE mol À1 for reaction (6). The molalities m in columns 3 to 5 are those obtained after mixing of the enzyme and substrate solutions and prior to any reaction.…”

“…chromatogram (see Section 2.2). The mass fraction of water in each of the nitrilase samples was determined by means of Karl Fischer titration [5].…”

A thermodynamic study of ketoreductase-catalyzed reactions 3. Reduction of 1-phenyl-1-alkanones in non-aqueous solvents

“…are given in table 1. The mass fraction of water listed in table 1 for the compounds involved in these reactions and the internal standard, 1-hexanol were determined by Karl Fischer titration [23]. The enzyme used in this study was ketoreductase (EC 1.1.1.2) from BioCatalytics, Inc., Pasadena, CA.…”

A thermodynamic study of ketoreductase-catalyzed reactions 2. Reduction of cycloalkanones in non-aqueous solvents

“…method described below and found to have purities greater than or equal to those stated by the vendors. The mass fractions of water given in table 1 for the compounds involved in these reactions and the internal standard, 1-hexanol, were determined by using Karl Fischer titration [21]. The ketoreductase (EC 1.1.1.2) used in this study was from Biocatalytics, Pasadena, CA.…”