Thermoanaerobacter ethanolicus gen. nov., spec. nov., a new, extreme thermophilic, anaerobic bacterium

Wiegel, Juergen; Ljungdahl, Lars G.

doi:10.1007/bf00405910

Cited by 262 publications

(172 citation statements)

References 19 publications

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“…Microbial strains and growth media T. ethanolicus JW200 was grown anaerobically in an 8 g/L glucose medium as described previously (Wiegel & Ljungdahl 1981). The novel expression plasmid pHsh, constructed 1054 Y.-H. Wang et al…”

Section: Methodsmentioning

confidence: 99%

Expression and characterization of an α-glucosidase from Thermoanaerobacter ethanolicus JW200 with potential for industrial application

et al. 2009

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In this study, a new α-glucosidase gene from Thermoanaerobacter ethanolicus JW200 was cloned and expressed in Escherichia coli by a novel heat-shock vector pHsh. The recombinant α-glucosidase exhibited its maximum hydrolytic activity at 70• C and pH 5.0∼5.5. With p-nitrophenyl-α-D-glucoside as a substrate and under the optimal condition (70 • C, pH 5.5), Km and Vmax of the enzyme was 1.72 mM and 39 U/mg, respectively. The purified α-glucosidase could hydrolyze oligosaccharides with both α-1,4 and α-1,6 linkages. The enzyme also had strong transglycosylation activity when maltose was used as sugar donor. The transglucosylation products towards maltose are isomaltose, maltotriose, panose, isomaltotriose and tetrasaccharides. The enzyme could convert 400 g/L maltose to oligosaccharides with a conversion rate of 52%, and 83% of the oligosaccharides formed were prebiotic isomaltooligosaccharides (containing isomaltose, panose and isomaltotriose).

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Section: Methodsmentioning

confidence: 99%

Expression and characterization of an α-glucosidase from Thermoanaerobacter ethanolicus JW200 with potential for industrial application

et al. 2009

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“…On the basis of its optimum growth temperature (55 to 60°C), which is the lowest optimum growth temperature among the Thermoanaerobacter strains that have been examined, and phenotypic characteristics, strain SEBR 5268T can be clearly differentiated from the seven previously validly described Thermoanaerobacter species (9,22,32,35,39) (Table 3). Strain SEBR 526gT differs from T. brockii (22,42) and T. ethanolicus (39) by its higher DNA G+C content.…”

Section: 4%) As Well As Thermoanaerobacter Acetoethylicus Thermomentioning

confidence: 99%

Description of Thermoanaerobacter brockii subsp. lactiethylicus subsp. nov., Isolated from a Deep Subsurface French Oil Well, a Proposal To Reclassify Thermoanaerobacter finnii as Thermoanaerobacter brockii subsp. finnii comb. nov., and an Emended Description of Thermoanaerobacter brockii

Cayol

Ollivier

Patel

et al. 1995

International Journal of Systematic Bacteriology

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“…However, very few microorganisms able to grow at such temperatures are able to generate ethanol from sugar (8)(9)(10), and no bacterium growing above 70°C produces an alcohol other than ethanol. In addition, no member of the domain Archaea is known to produce any alcohol as a major product, regardless of growth temperature.…”

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confidence: 99%

Single gene insertion drives bioalcohol production by a thermophilic archaeon

Basen

Schut

Nguyen

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Bioethanol production is achieved by only two metabolic pathways and only at moderate temperatures. Herein a fundamentally different synthetic pathway for bioalcohol production at 70°C was constructed by insertion of the gene for bacterial alcohol dehydrogenase (AdhA) into the archaeon Pyrococcus furiosus. The engineered strain converted glucose to ethanol via acetate and acetaldehyde, catalyzed by the host-encoded aldehyde ferredoxin oxidoreductase (AOR) and heterologously expressed AdhA, in an energy-conserving, redox-balanced pathway. Furthermore, the AOR/AdhA pathway also converted exogenously added aliphatic and aromatic carboxylic acids to the corresponding alcohol using glucose, pyruvate, and/or hydrogen as the source of reductant. By heterologous coexpression of a membrane-bound carbon monoxide dehydrogenase, CO was used as a reductant for converting carboxylic acids to alcohols. Redirecting the fermentative metabolism of P. furiosus through strategic insertion of foreign genes creates unprecedented opportunities for thermophilic bioalcohol production. Moreover, the AOR/AdhA pathway is a potentially game-changing strategy for syngas fermentation, especially in combination with carbon chain elongation pathways.Archaea | metabolic engineering | hyperthermophile | carbon monoxide | aldehydes

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Thermoanaerobacter ethanolicus gen. nov., spec. nov., a new, extreme thermophilic, anaerobic bacterium

Cited by 262 publications

References 19 publications

Expression and characterization of an α-glucosidase from Thermoanaerobacter ethanolicus JW200 with potential for industrial application

Expression and characterization of an α-glucosidase from Thermoanaerobacter ethanolicus JW200 with potential for industrial application

Description of Thermoanaerobacter brockii subsp. lactiethylicus subsp. nov., Isolated from a Deep Subsurface French Oil Well, a Proposal To Reclassify Thermoanaerobacter finnii as Thermoanaerobacter brockii subsp. finnii comb. nov., and an Emended Description of Thermoanaerobacter brockii

Single gene insertion drives bioalcohol production by a thermophilic archaeon

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