Thermoalkaliphilic laccase treatment for enhanced production of high-value benzaldehyde chemicals from lignin

Acidic Versus Alkaline Bacterial Degradation of Lignin Through Engineered Strain E. coli BL21(Lacc): Exploring the Differences in Chemical Structure, Morphology, and Degradation Products

“…The total concentration of PHA’s was 319.4 mg L –1 , with (S)-3-hydroxy-butanoic acid, 3-hydroxy-butanoic acid, and 3-hydroxybutyric acid in different percentages ( Shi et al, 2017 ). Further studies reviewed reported the identification of value-added compounds from lignin biodegradation by bacteria ( Duan et al, 2016 ; Suman et al, 2016 ; Zhu et al, 2017 ; Sapapporn et al, 2018 ; Shinoda et al, 2019 ; Yang et al, 2019 ). A thorough review of literature on lignin biodegradation identified the lack of research providing a systematic comparison of the lignin degradation in both sides of the pH spectrum using the same bacterial strain.…”

Applications of Microbial Laccases: Patent Review of the Past Decade (2009–2019)

“…Both processes, lignin removal and modification, now aim at the production of either small compounds as novel chemical building blocks, or at the development of new biomass-based materials. Recent examples include the production of benzaldehyde compounds and vanillin from lignin [78], the development of lignin-based controlled-release fertilizers [79], and the improvement of antioxidant activity of lignin [80], but also the modification of pulp for wood fiber insulation boards manufacturing [81]. Indeed, recent patents regarding the use of laccases in the paper industry are mostly dedicated to the incremental improvements of the established processes either through the addition of chemical mediators or the improved laccase variants (CN108823177A, CN109098025A).…”

Laccase-mediated synthesis of bioactive natural products and their analogues

“…34 Laccases are also employed in biorefineries to produce biofuels by degradation of lignocellulosic biomasses in the pulp and paper industry, 35,36 in the production of chemical building blocks from biomass-based materials and in the biodegradation of pesticides, pharmaceuticals and personalcare products (PPCPs). [37][38][39] These synthetic applications have highlighted that pH, solvent and chemical mediators can improve…”

“…34 Laccases are also employed in biorefineries to produce biofuels by degradation of lignocellulosic biomasses in the pulp and paper industry, 35,36 in the production of chemical building blocks from biomass-based materials and in the biodegradation of pesticides, pharmaceuticals and personal-care products (PPCPs). 37–39 These synthetic applications have highlighted that pH, solvent and chemical mediators can improve the enzyme efficiency, thus enabling laccases to oxidize a wider range of substrates in addition to phenolic compounds. Moreover, a wide number of supports, either inorganic, organic, or hybrids, have been employed to immobilize the enzyme, to overcome some practical limitations, 34,40 namely to increase the surface/volume ratio, thus avoiding the use of large amounts of enzyme for large-scale synthesis and allowing its effective recycling.…”